The RALF1-FERONIA complex phosphorylates FREE1 to attenuate abscisic acid signaling during seedling establishment DOI
Qiong Fu, Hongbo Li,

Bingqian Wang

и другие.

PLANT PHYSIOLOGY, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 23, 2024

Abstract The receptor-like kinase FERONIA (FER), together with its ligand rapid alkalinization factor 1 (RALF1) peptide, plays a crucial role in regulating stress responses, including involvement modulating abscisic acid (ABA) signaling. FER has been shown to activate ABA insensitive 2 the cytoplasm, leading suppression of However, regulation nucleus events response remains unclear. FREE1, identified as plant-specific component endosomal sorting complex required for transport eukaryotes, serves an important negative regulator In this study, we elucidate that upon RALF1 treatment, phosphorylates promoting accumulation FREE1 protein Arabidopsis (Arabidopsis thaliana). Consequently, suppresses sensitivity by inhibiting expression ABA-response genes. Mutating 6 phosphorylation sites on mediated FER, nonphosphorylable residues results reduced localization and increased hypersensitivity ABA. Our data also show these are likely involved plant survival under salt stress. Collectively, our study not only unveils additional function attenuating signaling but provides possible insight into RALF1-FER-FREE1 module coordinating growth tolerance.

Язык: Английский

Regulatory networks of bZIPs in drought, salt and cold stress response and signaling DOI
Yanli Yang, Yi Xu, Baozhen Feng

и другие.

Plant Science, Год журнала: 2025, Номер unknown, С. 112399 - 112399

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

1
Aquaporin CmPIP2; 3 links H2O2 signal and antioxidation to modulate trehalose-induced cold tolerance in melon seedlings DOI
Yuqing Han, Fei Luo, Adan Liang

и другие.

PLANT PHYSIOLOGY, Год журнала: 2024, Номер unknown

Опубликована: Сен. 9, 2024

Abstract Cold stress severely restricts the growth and development of cold-sensitive crops. Trehalose (Tre), known as “sugar life”, plays key roles in regulating plant cold tolerance by triggering antioxidation. However, relevant regulatory mechanism remains unclear. Here, we confirmed that Tre triggers apoplastic hydrogen peroxide (H2O2) production thus improving melon (Cucumis melo var. makuwa Makino) seedlings. Moreover, treatment can promote transport H2O2 to cytoplasm. This physiological process may depend on aquaporins. Further studies showed a Tre-responsive plasma membrane intrinsic protein 2;3 (CmPIP2;3) had strong function silencing CmPIP2;3 significantly weakened reduced Yeast library protein–DNA interaction technology were then used screen 2 transcription factors, abscisic acid–responsive element (ABRE)–binding factor (CmABF2) ABRE-binding 3 (CmABF3), which bind ABRE motif promoter activate its expression. Silencing CmABF2 CmABF3 further dramatically increased ratio H2O2/cytoplasm study uncovered induces CmABF2/3 positively regulate subsequently enhances seedlings promoting into cytoplasm for conducting redox signals stimulating downstream

Язык: Английский

Процитировано

7
Involvement of CONSTANS-like Proteins in Plant Flowering and Abiotic Stress Response DOI Open Access
Bingqian Zhang,

Minghui Feng,

Jun Zhang

и другие.

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(23), С. 16585 - 16585

Опубликована: Ноя. 22, 2023

The process of flowering in plants is a pivotal stage their life cycle, and the CONSTANS-like (COL) protein family, known for its photoperiod sensing ability, plays crucial role regulating plant flowering. Over past two decades, homologous genes COL have been identified various species, leading to significant advancements comprehending involvement pathway response abiotic stress. This article presents novel research progress on structural aspects proteins regulatory patterns within transcription complexes. Additionally, we reviewed recent information about participation stress response, aiming provide more comprehensive understanding functions proteins.

Язык: Английский

Процитировано

13
Comparative transcriptome and coexpression network analysis reveals key pathways and hub candidate genes associated with sunflower (Helianthus annuus L.) drought tolerance DOI Creative Commons
Huimin Shi,

Hou Jian-hua,

Dandan Li

и другие.

BMC Plant Biology, Год журнала: 2024, Номер 24(1)

Опубликована: Март 27, 2024

Abstract Background Drought severely limits sunflower production especially at the seedling stage. To investigate response mechanism of sunflowers to drought stress, we utilized two genotypes materials with different resistances as test materials. The physiological responses were investigated under well-watered (0 h) and drought-stressed conditions (24 h, 48 72 h). Results ANOVA revealed greatest differences in indices between h stress 0 stress. Transcriptome analysis was performed after At there 7482 5627 differentially expressed genes (DEGs) leaves K55 K58, respectively, 2150 2527 DEGs roots respectively. A total 870 transcription factors (TFs) identified among theDEGs, which high-abundance TF families included AP2/ERF, MYB, bHLH,and WRKY. Five modules screened using weighted gene coexpressionnetwork (WGCNA), three positively negatively, related traits. KEGG revealedthat “photosynthesis”, “carotenoid biosynthesis”, “starch sucrose metabolism”, “ribosome”, “protein phosphorylation” “phytohormone signaling” are six important metabolic pathways involved Cytoscape software used visualize key modules, hub screened. Finally, a 99 candidate that may be associated plants obtained, homology these compared Arabidopsis thaliana . Conclusions Taken together, our findings could lead better understanding tolerance facilitate selection drought-tolerant varieties.

Язык: Английский

Процитировано

4
Potential regulatory modules to integrate microgravity signals into flowering pathways in Arabidopsis thaliana grown in space DOI Creative Commons

Junyan Xie,

Jiachen Zhu, Lihua Wang

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Янв. 13, 2025

Abstract Our previous studies suggested that FLOWERING LOCUS (FT) can integrate microgravity signals into the photoperiodic flowering pathway in Arabidopsis. However, exact mechanisms remain unclear. In this study, we carried out transcriptomic analysis of wild-type (WT), gigantea (gi) mutant, CONSTANS overexpressing plants (35S:CO), and transgenic expressing FT with green fluorescent protein gene controlled by a heat shock-inducible promoter (pHSP:GFP;pHSP:FT, FG) grown on Chinese space station comparison their ground controls. The findings revealed transcriptional response signature was not only conserved primarily across GI-CO-FT module disruption genotypes WT, but also displayed GI-CO-FT specific associated responses. Transcription factors belong to ERF, bZIP, bHLH, BES1 families were significantly overrepresented GCC- CACGTG-elements responsive genes, suggesting possible role regulating time through integration pathways.

Язык: Английский

Процитировано

0
Genome-Wide Analysis of bZIP Transcription Factors and Expression Patterns in Response to Salt and Drought Stress in Vaccinium corymbosum DOI Open Access
Xinghua Feng, Chuchu Wang,

Sijin Jia

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(2), С. 843 - 843

Опубликована: Янв. 20, 2025

The basic leucine zipper (bZIP) transcription factors play essential roles in multiple stress responses and have been identified functionally characterized many plant species. However, the bZIP family members blueberry are unclear. In this study, we 102 VcbZIP genes Vaccinium corymbosum. VcbZIPs were divided into 10 groups based on phylogenetic analysis, each group shared similar motifs, domains, gene structures. Predictions of cis-regulatory elements upstream sequences indicated that proteins likely involved phytohormone signaling pathways abiotic responses. Analyses RNA deep sequencing data showed 18, 13, 7 differentially expressed response to salt, drought, ABA stress, respectively, for cultivar Northland. Ten responded both salt drought indicating unique overlapping signals. Of these genes, VcbZIP1–3 responsive abscisic acid treatments, their encoded may integrate signaling. Furthermore, from A VcbZIP83–84 VcbZIP75 S exhibited high or low expression under might be important regulators improving tolerance. Pearson correlation analyses revealed regulate stress-responsive improve tolerance a redundant manner. Our study provides useful reference functional blueberry.

Язык: Английский

Процитировано

0
Soybean RVE8a confers salt and drought tolerance in Arabidopsis DOI

Guohua Bao,

Guoqing Sun, Jingying Wang

и другие.

Biochemical and Biophysical Research Communications, Год журнала: 2024, Номер 704, С. 149660 - 149660

Опубликована: Фев. 28, 2024

Язык: Английский

Процитировано

3
Genome-wide identification of Shaker K+ channel family in Nicotiana tabacum and functional analysis of NtSKOR1B in response to salt stress DOI Creative Commons

Guang Yuan,

Tongjia Nong,

Oluwaseyi Setonji Hunpatin

и другие.

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Апрель 10, 2024

Soil salinization poses a mounting global ecological and environmental threat. The identification of genes responsible for negative regulation salt tolerance their utilization in crop improvement through gene editing technologies emerges as swift strategy the effective saline-alkali lands. One efficient mechanism plant is maintaining proper intracellular K + /Na ratio. Shaker channels play crucial role potassium absorption, transport, homeostasis cells. Here, study presents first genome-wide Nicotiana tabacum L., along with detailed bioinformatic analysis 20 identified members. Transcriptome revealed significant up-regulation NtSKOR1B , an outwardly-rectifying member predominantly expressed root tissue tobacco seedlings, response to stress. This finding was then confirmed by GUS staining ProNtSKOR1B::GUS transgenic lines RT-qPCR analysis. Subsequently, knockout mutants ( ntskor1 ) were generated subjected conditions. It found that exhibit enhanced tolerance, characterized increased biomass, higher content elevated ratios both leaf tissues, compared wild-type plants. These results indicate inhibits efflux tissues seedlings under stress, thereby within Thus, our identifies regulator seedlings.

Язык: Английский

Процитировано

2
Mechanism of auxin-dependent gene regulation through composite auxin response elements DOI

Daria D. Novikova,

Nadya Omelyanchuk,

Anastasiia Korosteleva

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июль 19, 2024

Abstract The plant signaling molecule auxin controls growth and development, largely through activating repressing the expression of thousands genes. Auxin-dependent transcriptional changes are mediated by DNA-binding Auxin Response Factors (ARF), whose AuxRE DNA binding sites well-known. identification first showed this to be part a composite element with second motif. Indeed, systematic analysis other motifs enriched in auxin-regulated promoters. Neither basis for enrichment nor mechanisms activity AuxRE’s is known. Here, we systematically mined Arabidopsis promoters elements auxin-responsive We identified many show that their presence reliable predictor response. Through mutating these higher-order modules, demonstrate function promoter activity. Lastly, transcription factors (TFs) bind AuxRE-associated motifs, involvement response, discovered several TFs directly ARF proteins. propose ARF-TF complexes specifically compound promoters, act as source diversification auxin-dependent gene regulation.

Язык: Английский

Процитировано

2
More than flowering: CONSTANS plays multifaceted roles in plant development and stress responses DOI Creative Commons
B. X. Yu, Yilong Hu, Xingliang Hou

и другие.

Journal of Integrative Plant Biology, Год журнала: 2024, Номер unknown

Опубликована: Окт. 28, 2024

Abstract Plants have evolved a remarkable ability to sense and respond changes in photoperiod, allowing adjustments their growth development based on seasonal environmental cues. The floral transition is pivotal stage plant development, signifying shift from vegetative reproductive growth. CONSTANS (CO), central photoperiodic response factor conserved various plants, mediates day‐length signals control the transition, although its mechanisms of action vary among plants with different requirements. In addition, recent studies uncovered roles for CO organ stress responses. These pleiotropic model crops make potentially fruitful target molecular breeding aimed at modifying crop agronomic traits. This review systematically traces research CO, discovery functional exploration regulatory newly discovered functions, providing important insight into laying foundation future research.

Язык: Английский

Процитировано

2