Exploring the multi-targeted mechanism of Saikosaponin A in prostate cancer treatment: a network pharmacology and molecular docking approach
Frontiers in Pharmacology,
Год журнала:
2025,
Номер
16
Опубликована: Фев. 10, 2025
Background
Prostate
cancer
(PCa)
is
one
of
the
prevalent
malignant
tumors
among
men.
It
can
progress
to
castration-resistant
prostate
(CRPC),
which
significantly
more
challenging
treat.
Saikosaponin
A
(SSA),
a
triterpenoid
saponin
extracted
from
genus
Bupleurum,
exerts
numerous
pharmacological
effects,
including
anti-inflammatory
and
anti-tumorigenic
effects.
However,
mechanism
underlying
effects
SSA
in
treatment
remains
elusive.
Methods
In
this
study,
network
pharmacology
approach
was
applied
identify
relevant
targets
drug-
disease-related
databases,
intersections
were
analyzed
using
Venny2.1
construct
Protein-Protein
interaction
(PPI)
network.
Next,
Gene
Ontology
(GO)
Kyoto
Encyclopedia
Genes
Genomes
(KEGG)
enrichment
analyses
performed
elucidate
associated
biological
functions
signaling
pathways.
Meanwhile,
molecular
docking
between
core
Autodock
software.
Lastly,
vitro
experiments
for
validation.
Results
least
four
key
targets,
namely
BCL2
apoptosis
regulator
(BCL2),
estrogen
receptor
1
(ESR1),
hypoxia-inducible
factor
subunit
alpha
(HIF1A),
signal
transducer
activator
transcription
3
(STAT3)
identified
revealed
that
stably
binds
these
targets.
Moreover,
results
inhibited
proliferative
migratory
abilities
PC3
cells
dose-dependent
manner.
Finally,
also
induced
G1-phase
blockade
cells,
further
highlighting
its
potential
role
treatment.
Conclusion
The
present
study
by
targeting
multiple
pathways,
laying
theoretical
reference
use
as
therapeutic
candidate
cancer.
Язык: Английский
N6-methyladenosine-mediated upregulation of H19 promotes resistance to bortezomib by modulating the miR-184/CARM1 axis in multiple myeloma
Clinical and Experimental Medicine,
Год журнала:
2025,
Номер
25(1)
Опубликована: Апрель 1, 2025
Язык: Английский
Integrative Multi-Omics Analysis Reveals Molecular Subtypes of Ovarian Cancer and Constructs Prognostic Models
Journal of Immunotherapy,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 9, 2025
Summary:
Ovarian
cancer
(OV)
remains
the
most
lethal
gynecological
malignancy.
The
aim
of
this
study
was
to
identify
molecular
subtypes
OV
through
integrative
multi-omics
analysis
and
construct
machine
learning-based
prognostic
models
for
predicting
efficacy
immunotherapy.
In
here,
mutation,
copy
number
variation,
RNA
sequencing
expression
profiles,
clinical
information
were
obtained
from
Cancer
Genome
Atlas
(TCGA)
Gene
Expression
Omnibus
(GEO)
databases.
Multi-omics
data
stratified
using
MOVICS
package,
identifying
different
subtypes.
Our
identified
2
(CS1
CS2)
with
significant
survival
differences.
Transcriptional
regulatory
network
revealed
differential
activation
transcription
factors
such
as
FOXA1
GATA3
in
CS1,
whereas
AR
ESR2
enriched
CS2.
A
robust
signature
comprising
5
key
genes
developed
integration
10
learning
algorithms,
demonstrating
high
predictive
power
across
sets.
Immune
cell
infiltration
that
anti-tumor
immune
cells
more
abundant
low-risk
groups,
pro-tumor
predominated
high-risk
groups.
Furthermore,
patients
exhibited
better
immunotherapy
responses
higher
tumor
mutational
burden
(TMB).
conclusion,
our
findings
underscore
potential
unveiling
novel
constructing
inform
personalized
treatment
strategies.
Future
research
should
focus
on
validating
these
larger
cohorts
enhance
management
targeted
therapeutic
approaches.
Язык: Английский
SLC7A5: A Potential Molecular Target for the Diagnosis and Treatment of TNBC
BIO Web of Conferences,
Год журнала:
2025,
Номер
174, С. 02021 - 02021
Опубликована: Янв. 1, 2025
TNBC
is
a
highly
heterogeneous
malignant
tumor
with
limited
clinical
treatment
options
and
efficacy.
Through
series
of
analyses
screenings,
we
have
identified
SLC7A5
as
potential
biomarker
for
targeted
therapy
TNBC.
Experimental
results
shown
that
knocking
down
can
significantly
inhibit
the
proliferation
TNBC,
also
quercetin,
Chinese
herbal
ingredient
lower
cost
efficacy
comparable
to
existing
specific
inhibitor
JPH-203.
quercetin
Our
research
provides
new
ideas
lessons
treatment.
Язык: Английский
Implications of ITCH-mediated ubiquitination of SIX1 on CDC27-cyclinB1 signaling in nasopharyngeal carcinoma
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Окт. 15, 2024
Nasopharyngeal
carcinoma
(NPC)
presents
a
significant
medical
challenge
due
to
its
high
incidence
rate
and
poor
prognosis,
which
are
attributed
primarily
tumor
metastasis
drug
resistance.
Sine
oculis
homeobox
homolog
1
(SIX1)
has
been
identified
as
crucial
target
for
cancer
treatment.
However,
role
in
NPC
remains
incompletely
understood.
This
study
investigated
the
mechanisms
by
degradation
of
SIX1
protein,
is
mediated
ubiquitin,
affects
malignant
characteristics
throughout
cell
cycle.
Our
findings
reveal
that
reduced
expression
itchy
E3
ubiquitin
ligase
(ITCH)
impedes
leading
enhance
oncogenic
properties.
Knockdown
experiments
was
inhibited
demonstrated
decrease
proliferation,
migration,
invasion
lines,
whereas
overexpression
yielded
opposite
effects.
Further
experimental
validation
revealed
promotes
progression
via
division
cycle
27
(CDC27)/cyclin
B1
axis.
These
provide
valuable
insights
into
potential
therapeutic
targets
prognostic
indicators
treatment,
emphasizing
ITCH/SIX1/CDC27/cyclin
axis
promising
novel
therapies.
Язык: Английский
USP4/CARM1 axis promotes the malignant transformation of breast cancer cells by upregulating SLC7A11 expression
Clinical Breast Cancer,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 1, 2024
Язык: Английский
Methylation modification of non-histone proteins in breast cancer: An emerging targeted therapeutic strategy
Pharmacological Research,
Год журнала:
2024,
Номер
208, С. 107354 - 107354
Опубликована: Авг. 17, 2024
Breast
cancer
is
a
major
public
health
concern
worldwide,
being
the
most
commonly
diagnosed
among
women
and
leading
cause
of
cancer-related
deaths.
Recent
studies
have
highlighted
significance
non-histone
methylation
in
breast
cancer,
which
modulates
activity,
interaction,
localization,
stability
target
proteins.
This
regulation
affects
critical
processes
such
as
oncogenesis,
tumor
growth,
proliferation,
invasion,
migration,
immune
responses.
review
delves
into
enzymes
responsible
for
methylation,
protein
arginine
methyltransferases
(PRMTs),
lysine
(KMTs),
demethylases,
explores
their
roles
cancer.
By
elucidating
molecular
mechanisms
functional
consequences
this
aims
to
provide
insights
novel
therapeutic
strategies
targeting
these
pathways.
The
potential
overcome
drug
resistance
enhance
treatment
efficacy
also
discussed,
highlighting
promising
avenues
future
research
clinical
applications.
Язык: Английский
C/EBPβ Regulates HIF-1α-Driven Invasion of Non-Small-Cell Lung Cancer Cells
Biomolecules,
Год журнала:
2024,
Номер
15(1), С. 36 - 36
Опубликована: Дек. 30, 2024
Metastatic
cancer
accounts
for
most
cancer-related
deaths,
and
identifying
specific
molecular
targets
that
contribute
to
metastatic
progression
is
crucial
the
development
of
effective
treatments.
Hypoxia,
a
feature
solid
tumors,
plays
role
in
by
inducing
resistance
therapy
accelerating
metastasis.
Here,
we
report
CCAAT/enhancer-binding
protein
beta
(C/EBPβ)
transcriptionally
regulates
hypoxia-inducible
factor
1
subunit
alpha
(HIF1A)
thus
promotes
migration
invasion
non-small-cell
lung
(NSCLC)
cells
under
hypoxic
conditions.
Our
results
show
knockdown
or
forced
expression
C/EBPβ
was
correlated
with
HIF-1α
directly
bound
promoter
region
HIF1A.
Silencing
HIF1A
inhibited
enhanced
induced
overexpression
NSCLC
hypoxia.
Expression
target
gene,
SLC2A1,
also
altered
C/EBPβ-dependent
manner,
SLC2A1
reduced
overexpression.
These
indicate
critical
regulator
tumor
microenvironment.
Collectively,
C/EBPβ-HIF-1α-GLUT1
axis
represents
potential
therapeutic
preventing
improving
patient
outcomes.
Язык: Английский