A barley MLA immune receptor is activated by a fungal nonribosomal peptide effector for disease susceptibility DOI
Yueqiang Leng, Florian Kümmel, Mingxia Zhao

и другие.

New Phytologist, Год журнала: 2024, Номер unknown

Опубликована: Дек. 6, 2024

Summary The barley Mla locus contains functionally diversified genes that encode intracellular nucleotide‐binding leucine‐rich repeat receptors (NLRs) and confer strain‐specific immunity to biotrophic hemibiotrophic fungal pathogens. In this study, we isolated a gene Scs6 , which is an allelic variant of but confers susceptibility the isolate ND90Pr ( Bs ) necrotrophic fungus Bipolaris sorokiniana . We generated transgenic lines showed sufficient in genotypes naturally lacking receptor. Scs6‐ encoded NLR (SCS6) activated by nonribosomal peptide (NRP) effector produced induce cell death Nicotiana benthamiana Domain swaps between MLAs SCS6 reveal domain specificity determinant for receptor activation NRP effector. maintained both wild domesticated populations. Our phylogenetic analysis suggests Hordeum ‐specific innovation. infer bona fide immune likely directly disease barley. study provides stepping stone future development synthetic crops are less vulnerable modification

Язык: Английский

Pathogen perception and signaling in plant immunity DOI Creative Commons
Peter N. Dodds, Jian Chen, Megan A. Outram

и другие.

The Plant Cell, Год журнала: 2024, Номер 36(5), С. 1465 - 1481

Опубликована: Янв. 23, 2024

Abstract Plant diseases are a constant and serious threat to agriculture ecological biodiversity. Plants possess sophisticated innate immunity system capable of detecting responding pathogen infection prevent disease. Our understanding this has grown enormously over the past century. Early genetic descriptions plant disease resistance virulence were embodied in gene-for-gene hypothesis, while physiological studies identified pathogen-derived elicitors that could trigger defense responses cells tissues. Molecular these phenomena have now coalesced into an integrated model involving cell surface intracellular detection specific molecules proteins culminating induction various cellular responses. Extracellular receptors engage distinct signaling processes but converge on many similar outputs with substantial evidence for integration pathways interdependent networks controlling outcomes. Many molecular details recognition known, providing opportunities bioengineering enhance protection from Here we provide overview current main principles immunity, emphasis key scientific milestones leading insights.

Язык: Английский

Процитировано

36

Substrate-induced condensation activates plant TIR domain proteins DOI Creative Commons
Wen Song,

Li Liu,

Dongli Yu

и другие.

Nature, Год журнала: 2024, Номер 627(8005), С. 847 - 853

Опубликована: Март 13, 2024

Abstract Plant nucleotide-binding leucine-rich repeat (NLR) immune receptors with an N-terminal Toll/interleukin-1 receptor (TIR) domain mediate recognition of strain-specific pathogen effectors, typically via their C-terminal ligand-sensing domains 1 . Effector binding enables TIR-encoded enzymatic activities that are required for TIR–NLR (TNL)-mediated immunity 2,3 Many truncated TNL proteins lack effector-sensing but retain similar and 4,5 The mechanism underlying the activation these TIR remain unclear. Here we show substrates NAD + ATP induces phase separation in vitro. A condensation occurs a protein expressed its native promoter response to inoculation planta. formation condensates is mediated by conserved self-association interfaces predicted intrinsically disordered loop region TIRs. Mutations disrupt impair cell death activity proteins. Our data reveal as provide insight into substrate-induced autonomous signalling confer plant immunity.

Язык: Английский

Процитировано

22

Plant NLR immunity activation and execution: a biochemical perspective DOI Creative Commons
Federica Locci, Jane E. Parker

Open Biology, Год журнала: 2024, Номер 14(1)

Опубликована: Янв. 1, 2024

Plants deploy cell-surface and intracellular receptors to detect pathogen attack trigger innate immune responses. Inside host cells, families of nucleotide-binding/leucine-rich repeat (NLR) proteins serve as sensors or downstream mediators defence outputs cell death, which prevent disease. Established genetic underpinnings NLR-mediated immunity revealed various strategies plants adopt combat rapidly evolving microbial pathogens. The molecular mechanisms NLR activation signal transmission components controlling execution were less clear. Here, we review recent protein structural biochemical insights plant sensor signalling functions. When put together, the data show how different families, whether transducers, converge on nucleotide-based second messengers cellular calcium confer immunity. Although pathogen-activated NLRs in engage plant-specific machineries promote defence, comparisons with mammalian receptor counterparts highlight some shared working principles for across kingdoms.

Язык: Английский

Процитировано

20

Balanced plant helper NLR activation by a modified host protein complex DOI
Shijia Huang, Junli Wang, Ruirui Song

и другие.

Nature, Год журнала: 2025, Номер unknown

Опубликована: Фев. 12, 2025

Язык: Английский

Процитировано

3

Small holes, big impact: Stomata in plant–pathogen–climate epic trifecta DOI Creative Commons
Shuguo Hou, Olivier Rodrigues, Zunyong Liu

и другие.

Molecular Plant, Год журнала: 2023, Номер 17(1), С. 26 - 49

Опубликована: Дек. 1, 2023

Язык: Английский

Процитировано

35

Natural variation in the pattern‐triggered immunity response in plants: Investigations, implications and applications DOI Creative Commons
Asher I. Hudson,

Alexander Mullens,

Sarah R. Hind

и другие.

Molecular Plant Pathology, Год журнала: 2024, Номер 25(3)

Опубликована: Март 1, 2024

Abstract The pattern‐triggered immunity (PTI) response is triggered at the plant cell surface by recognition of microbe‐derived molecules known as microbe‐ or pathogen‐associated molecular patterns derived from compromised host cells called damage‐associated patterns. Membrane‐localized receptor proteins, pattern receptors, are responsible for this recognition. Although much machinery PTI conserved, natural variation exists within and across species with respect to components recognition, activation response, strength induced. This review describes what about variation. We discuss how in can be measured knowledge might utilized control disease developing varieties enhanced resistance.

Язык: Английский

Процитировано

13

MAPK Cascades in Plant Microbiota Structure and Functioning DOI
Thijs Van Gerrewey, Hoo Sun Chung

The Journal of Microbiology, Год журнала: 2024, Номер 62(3), С. 231 - 248

Опубликована: Март 1, 2024

Язык: Английский

Процитировано

8

New insight into Ca2+‐permeable channel in plant immunity DOI
Wei Wang, Hang‐Yuan Cheng, Jian‐Min Zhou

и другие.

Journal of Integrative Plant Biology, Год журнала: 2024, Номер 66(3), С. 623 - 631

Опубликована: Янв. 30, 2024

ABSTRACT Calcium ions (Ca 2+ ) are crucial intracellular second messengers in eukaryotic cells. Upon pathogen perception, plants generate a transient and rapid increase cytoplasmic Ca levels, which is subsequently decoded by sensors effectors to activate downstream immune responses. The elevation of cytosolic commonly attributed influx mediated plasma membrane‐localized –permeable channels. However, the contribution release triggered ‐permeable channels shaping signaling associated with plant immunity remains poorly understood. This review discusses recent advances understanding mechanism underlying signatures upon activation receptors, particular emphasis on identification receptors as non‐canonical We also discuss involvement from endoplasmic reticulum generating during immunity.

Язык: Английский

Процитировано

7

The barley MLA13-AVRA13heterodimer reveals principles for immunoreceptor recognition of RNase-like powdery mildew effectors DOI
Aaron W. Lawson, Andrea Flores-Ibarra, Yu Cao

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июль 17, 2024

Abstract Co-evolution between cereals and pathogenic grass powdery mildew fungi is exemplified by sequence diversification of an allelic series barley resistance genes encoding Mildew Locus A (MLA) nucleotide-binding leucine-rich repeat (NLR) immunoreceptors with a N-terminal coiled-coil domain (CNLs). Each immunoreceptor recognises matching, strain-specific effector encoded avirulence gene ( AVR ) . We present here the cryo-EM structure MLA13 in complex its cognate A13 -1. The adopts RNase-like fold when bound to planta , similar crystal structures other e ffectors purified from E. coli -1 interacts via basal loops C-terminal leucine rich repeats (LRRs) central winged helix (WHD). Co-expression structure-guided substitution variants show that receptor–effector interface plays essential role mediating immunity-associated plant cell death. Furthermore, combining structural information MLA13–AVR heterocomplex alignments MLA receptors, we designed single amino acid MLA7 enables expanded detection virulent variant -V2. In contrast pentameric conformation previously reported effector-activated CNL resistosomes, was resolved as stable heterodimer expression system. Our study suggests might represent output distinct resistosomes highlights opportunities for development designer gain-of-function NLRs.

Язык: Английский

Процитировано

7

From molecule to cell: the expanding frontiers of plant immunity DOI
Lei Li, J. Liu, Jian‐Min Zhou

и другие.

Journal of genetics and genomics/Journal of Genetics and Genomics, Год журнала: 2024, Номер 51(7), С. 680 - 690

Опубликована: Фев. 26, 2024

Язык: Английский

Процитировано

5