Desert plants, arbuscular mycorrhizal fungi and associated bacteria: Exploring the diversity and role of symbiosis under drought

Environmental Microbiology Reports, Год журнала: 2024, Номер 16(4)

Опубликована: Июль 9, 2024

Desert plants, such as Agave tequilana, A. salmiana and Myrtillocactus geometrizans, can survive harsh environmental conditions partly due to their symbiotic relationships with microorganisms, including arbuscular mycorrhizal fungi (AMF). Interestingly, some of these also harbour endosymbiotic bacteria. Our research focused on investigating the diversity AMFs associated bacteria in plants growing arid soil. We found that agaves have a threefold higher AMF colonization than M. geometrizans. Metabarcoding techniques revealed composition communities was primarily influenced by plant host, while bacterial were more affected specific compartment or niche they inhabited. identified both known novel endofungal taxa, Burkholderiales, confirmed presence within spores using multiphoton microscopy. study explored effects drought symbiosis between tequilana AMF. discovered severity could modulate strength this its outcomes for holobiont. Severe prevented formation symbiosis, moderate promoted it, thereby conferring tolerance tequilana. This sheds light Crassulacean Acid Metabolism (CAM) underscores crucial role factor modulating Further is needed understand response.

Язык: Английский

---

A cross-systems primer for synthetic microbial communities

Nature Microbiology, Год журнала: 2024, Номер 9(11), С. 2765 - 2773

Опубликована: Окт. 30, 2024

Язык: Английский

---

Plant Probiotic Potential of Native Rhizobia to Enhance Growth and Sugar Content in Agave tequilana Weber var. Blue

Horticulturae, Год журнала: 2025, Номер 11(2), С. 137 - 137

Опубликована: Янв. 28, 2025

Beneficial soil microorganisms, particularly plant probiotic bacteria (PPB), play a pivotal role in promoting growth, development, and overall health through root colonization. PPB-based biofertilizers offer sustainable eco-friendly alternative to conventional agricultural inputs. This study evaluates the potential of three native bacterial strains Rhizobium sp. ACO-34A, Sinorhizobium mexicanum ITTG R7T, chiapasense S70T enhance quality, sugar content Agave tequilana. A comprehensive genomic functional analysis was conducted for each strain assess their traits. Additionally, greenhouse inoculation assay performed on six-month-old agave seedlings at “piña” stage evaluate effects these growth content. Comparative revealed that rhizobial harbor genes associated with key traits, reinforcing enhancing development. The results demonstrated significant (p < 0.05) inoculated plants. ACO-34A increased height by 35.4%, fresh weight 41.5%, inulin 57.3%, while ITTG-R7T showed improvements 26.4%, 35.2%, 38.2%, respectively, compared control, also exhibited enhancements, although lesser extent, increases 23.5% height, 28.9% weight, 31.2% These findings highlight biofertilizer strains, positioning them as promising candidates cultivation A.

Язык: Английский

---

Chronic Exposure to Petroleum-Derived Hydrocarbons Alters Human Skin Microbiome and Metabolome Profiles: A Pilot Study

Journal of Proteome Research, Год журнала: 2024, Номер 23(10), С. 4273 - 4285

Опубликована: Июль 18, 2024

Petroleum-derived substances, like industrial oils and grease, are ubiquitous in our daily lives. Comprised of petroleum hydrocarbons (PH), these substances can come into contact with skin, potentially causing molecular disruptions contributing to the development chronic disease. In this pilot study, we employed mass spectrometry-based untargeted metabolomics 16S rRNA gene sequencing analyses explore effects. Superficial skin samples were collected from subjects without dermal exposure PH at two anatomical sites: fingers (referred as hand) arms (serving an intersubject variability control). Exposed hands exhibited higher bacterial diversity (Shannon Simpson indices) enrichment oil-degrading bacteria (ODB), including

Язык: Английский

---

A stable 15-member bacterial SynCom promotes Brachypodium growth under drought stress

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Сен. 10, 2024

Abstract Rhizosphere microbiomes are known to drive soil nutrient cycling and influence plant fitness during adverse environmental conditions. Field-derived robust Synthetic Communities (SynComs) of microbes that mimic the diversity rhizosphere can greatly advance a deeper understanding such processes. However, assembling stable, genetically tractable, reproducible, scalable SynComs remains challenging. Here, we present systematic approach using combination network analysis cultivation-guided methods construct 15-member SynCom from rhizobiome Brachypodium distachyon . This incorporates diverse strains five bacterial phyla demonstrates strong stability both in vitro planta Genomic individual revealed they encode multiple growth-promoting traits, some which were validated by laboratory phenotypic assays. Additionally, most encoded genes for synthesis osmoprotectants (trehalose betaine) Na + /K transporters. These traits likely enabled resilience drought stress where plants amended with recovered better than without. We further observed preferential colonization around root tips under stress, due active interactions between metabolites bacteria. Our results represent significant progress towards building testing stable model plant-microbe interactions.

Язык: Английский

---

Richness and composition of phyllosphere Methylobacterium communities cause variation in Arabidopsis thaliana growth

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Сен. 2, 2024

ABSTRACT The phyllosphere – the aerial parts of plants forms a vast microbial habitat that harbors diverse bacterial communities playing key roles in ecosystem function. foliar surface is thus promising study system to investigate biodiversity-ecosystem function relationships. Researchers have found positive correlation between leaf diversity and productivity, but causality this relationship has yet be demonstrated. To understand how composition could cause variation growth their host plants, we assembled synthetic composed different compositions Methylobacterium strains plant growth-promoting genus ubiquitous inoculated on Arabidopsis thaliana grown gnotobiotic conditions. We hypothesized (1) increasing should an increase growth; (2) differ impact (3) productivity strain-dependent. Our results supported our three hypotheses revealed unpredicted patterns A. biomass varied according strain richness identity. Increasing induced higher biomass, only after initial reduction suggesting competition alleviation by multispecies interactions. Two showed beneficial effects growth, one was detrimental for plant. Community shaped highlighting importance community mutualistic antagonistic Furthermore, niche complementarity likely main ecological mechanism driving diversity-productivity system. By demonstrating causal experiment shed light bacteria terrestrial functioning.

Язык: Английский

---

The Agave and Cacti microbiome: Models for a planet under global warming

Elsevier eBooks, Год журнала: 2024, Номер unknown, С. 421 - 460

Опубликована: Ноя. 8, 2024

Язык: Английский

---

The Colonization of Synthetic Microbial Communities Carried by Bio-Organic Fertilizers in Continuous Cropping Soil for Potato Plants

Microorganisms, Год журнала: 2024, Номер 12(11), С. 2371 - 2371

Опубликована: Ноя. 20, 2024

Synthetic microbial communities (SynComs) play significant roles in soil health and sustainable agriculture. In this study, bacterial SynComs (SCBs) fungal (SCFs) were constructed by selecting species that could degrade the potato root exudates associated with continuous cropping obstacles. SCBs, SCFs, SCB + SCF combinations then inoculated into organic fertilizers (OFs, made from sheep manure) to produce three bio-organic (BOFs), denoted SBFs (BOFs of SCBs), SFFs SCFs), SBFFs combinations), respectively. The OF BOFs, a chemical fertilizer (CK) as control, used pot experiments involving growth 4-year field. Microbial diversity sequencing was investigate colonization SCBs SCFs rhizosphere bulk soil, their effects on evaluated. Source Tracker analysis showed increased but at relatively low level 1% total bacteria, while fungi SFF much higher 5-18% fungi. combination, significantly SBFF both soil. Overall, more susceptible influence BOFs than bacteria. general, application did not change alpha diversity. Correlation network key bacteria stable soils different groups, especially changed among groups. LEfSe activated some rare species, which correlated improvements function categories tolerance stress, nitrogen fixation, saprotroph functions. Mantel test affected physicochemical properties, influencing core promoting growth. It also noted presence SynCom-inoculated may lead slight increase plant pathogens, needs be considered optimization SynCom applications overcome obstacles production.

Язык: Английский

---

Bacterial community function increases leaf growth in a pitcher plant experimental system

mSystems, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 25, 2024

ABSTRACT Across diverse ecosystems, bacteria and their hosts engage in complex relationships having negative, neutral, or positive interactions. However, the specific effects of leaf-associated bacterial community functions on plant growth are poorly understood. Although microbes can promote through various biochemical mechanisms, investigating community’s functional contributions to remains be explored. To address this gap, we characterized between function host purple pitcher ( Sarracenia purpurea ). The main aim our research was investigate how different affect nutrient content plant. Previous has suggested that microbial communities aid prey decomposition subsequent acquisition carnivorous plants, including S. . roles uptake not well known. In study, sterile, freshly opened pitchers were inoculated with three functionally distinct, pre-assembled communities. Bacterial composition measured over 8 weeks using physiological assays, metagenomics, metatranscriptomics. Distinct affected traits; a enriched associated larger leaves almost double biomass control pitchers. Physiological differences supported by metatranscriptomics; for example, highest chitinase activity had greater expression transcripts enzymes. relationship observed here indicates potential such as activity, host-associated support growth. IMPORTANCE This study addresses gap understanding We distinct uncover mechanisms which health Our findings demonstrate significantly influence traits, some supporting more than These results highlight ecological plants thus ecosystems suggest cycling is an important pathway health. provides valuable insights into plant-microbe interactions functions.

Язык: Английский

---

Agricultural practices and environmental factors drive microbial communities in the mezcal-producing Agave angustifolia Haw.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Дек. 5, 2024

Mezcal, a traditional Mexican alcoholic beverage, has been vital source of livelihood for indigenous and rural communities centuries. However, increasing international demand is exerting pressure on natural resources encouraging intensive agricultural practices. This study investigates the impact management practices (wild, traditional, conventional) environmental factors microbial associated with Agave angustifolia, key species in mezcal production. High-throughput sequencing 16S rRNA ITS2 gene regions revealed distinct prokaryotic fungal community structures across different plant compartments (endosphere, episphere, soil), identifying 8,214 7,459 ASVs. Core were dominated by Proteobacteria, Actinobacteria, Ascomycota, Basidiomycota. Alpha diversity analyses showed significant increases from endosphere to soil, while remained stable. Notably, conventional reductions beneficial taxa. Environmental such as precipitation temperature significantly influenced composition, especially rhizosphere. Beta patterns underscored strong compartment, aridity further shaping communities. These results reveal intricate interactions between practices, conditions, diversity, providing valuable insights sustainable cultivation A. angustifolia.

Язык: Английский

---