TheFusarium graminearumeffector protease FgTPP1 suppresses immune responses and facilitates Fusarium Head Blight Disease
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 2, 2024
ABSTRACT
Most
plant
pathogens
secrete
effector
proteins
to
circumvent
host
immune
responses,
thereby
promoting
pathogen
virulence.
One
such
is
the
fungus
Fusarium
graminearum
,
which
causes
Head
Blight
(FHB)
disease
on
wheat
and
barley.
Transcriptomic
analyses
revealed
that
F.
expresses
many
candidate
during
early
phases
of
infection
process,
some
are
annotated
as
proteases.
However,
contributions
these
proteases
virulence
remains
poorly
defined.
Here,
we
characterize
a
endopeptidase,
FgTPP1
(FGSG_11164),
highly
upregulated
spikelet
secreted
from
fungal
cells.
To
elucidate
potential
role
in
virulence,
generated
deletion
mutants
(
ΔFgtpp1
)
performed
FHB
assays.
While
number
completely
bleached
spikes
infected
by
F
.
wild-type
reached
50%
total
spikes,
fully
was
25%,
suggesting
contributes
Transient
expression
green
fluorescent
protein
(GFP)-tagged
localizes,
part,
chloroplasts
attenuates
chitin-mediated
activation
mitogen-activated
kinase
(MAPK)
signaling,
reactive
oxygen
species
production,
cell
death
induced
an
autoactive
resistance
when
expressed
planta
Notably,
conserved
across
Ascomycota
phylum,
making
it
core
among
ascomycete
pathogens.
These
properties
make
ideal
for
decoy
substrate
engineering,
with
goal
engineering
FHB,
likely
other
crop
diseases
caused
fungi.
Язык: Английский
Use of the Puccinia sorghi haustorial transcriptome to identify and characterize AvrRp1-D recognized by the maize Rp1-D resistance protein
PLoS Pathogens,
Год журнала:
2024,
Номер
20(11), С. e1012662 - e1012662
Опубликована: Ноя. 8, 2024
The
common
rust
disease
of
maize
is
caused
by
the
obligate
biotrophic
fungus
Puccinia
sorghi
.
Rp1-D
allele
imparts
resistance
against
P
IN2
isolate
initiating
a
defense
response
that
includes
rapid
localized
programmed
cell
death
process,
hypersensitive
(HR).
In
this
study,
to
identify
AvrRp1-D
from
IN2,
we
employed
isolation
haustoria,
facilitated
biotin-streptavidin
interaction,
as
powerful
approach.
This
method
proves
particularly
advantageous
in
cases
where
genome
information
for
fungal
pathogen
unavailable,
enhancing
our
ability
explore
and
understand
molecular
interactions
between
haustorial
transcriptome
generated
through
technique,
combination
with
bioinformatic
analyses
such
SignalP
TMHMM,
enabled
identification
251
candidate
effectors.
We
ultimately
identified
two
closely
related
genes,
1
2
,
which
triggered
an
-dependent
Nicotiana
benthamiana
-induced
HR
was
further
confirmed
protoplasts.
demonstrated
AvrRp1-D.1
interacts
directly
specifically
leucine-rich
repeat
(LRR)
domain
yeast
two-hybrid
assay.
also
provide
evidence
that,
absence
Rp1-D,
plays
role
suppressing
plant
immune
response.
Our
research
provides
valuable
insights
into
driving
maize.
Язык: Английский