Physiological and developmental disturbances caused by Botryosphaeria dieback in the annual stems of grapevine DOI Creative Commons

Florian Moret,

Lucile Jacquens,

Philippe Larignon

и другие.

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Апрель 23, 2024

Botryosphaeria dieback is a grapevine trunk disease caused by fungi of the Botryosphaeriaceae family, which attacks more specifically woody tissues. The infection leads to different symptoms including severe form with leaf drop as well premature plant death. causes major economic losses, since no effective treatment yet available. A better understanding necessary find solutions fight this disease. In study, our objective was characterize “leaf drop” (1) looking for presence pathogens in basal internodes stems, (2) quantifying blocked vessels tylosis and/or gummosis, and (3) describing impact on vine physiology (gene expression metabolome) development (establishment functioning cambium phellogen) at level xylem phloem stem internodes. Our study has shown that were present both annual stem, obturated. We have also clearly demonstrated gene metabolite profiles strongly modified diseased plants. Differences stems between healthy (control, C) (D) plants low flowering (vines not symptomatic), higher onset symptom still present, although less marked, full expression. qRT-PCR analysis showed an overexpression genes involved defense, repression related meristematic activity (i.e. vascular phellogen). Metabolomic specific fingerprints from expression, increase phytoalexins mannitol, decrease 1-kestose one. At structural level, many alterations observed internodes, even before symptoms: classical obstruction and, first time, disorganization secondary sieve plates callose. modifies (liber) phellogen. Altogether, combining approaches allowed highlight deep dysfunction base may explain decline due dieback.

Язык: Английский

Rhizobacteria from vineyard and commercial mycorrhizal fungi induce synergistic microbiome shifts within grapevine root systems DOI Creative Commons
Romain Darriaut,

Vincent Lailheugue,

Jules Wastin

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Янв. 28, 2025

Abstract Background The addition of bacteria and arbuscular mycorrhizal fungi (AMF) is a strategy used to protect plants against disease improve their growth yield, known as biocontrol biostimulation, respectively. In viticulture, the plant promotion (PGP) potential endemic vineyard soil has been underexplored. Furthermore, most research about microbial biostimulants focuses on effect plant, but little how application modify root ecosystem, which may have an impact resistance. objectives this work were 1) identify present in soils with functional PGP traits, 2) test activity young grapevines, combination AMF, 3) assess communities inferred functions rhizosphere roots. Results Two hundred isolated from vineyards characterized for biochemical activities. efficient tested in vitro, both singly combination, Lepidium sativum grapevine plantlets. Pseudomonas species particularly increased vitro selected further testing, without two Glomus species, grapevines planted experiencing dysbiosis greenhouse setting. After five months growth, co-application rhizobacteria AMF significantly enhanced biomass abundance potentially beneficial bacterial genera roots, compared untreated conditions single inoculum treatments. Additionally, prevalence Botrytis cinerea, associated diseases, decreased endosphere. combined inoculation resulted more complex network higher metabolic functionality than Conclusions To our knowledge, first study examine apply strains derived same plot commercialized fungi. results show remodeling terms presence pathogens. observed synergistic indicates that it important consider effects individuals synthetic applied field.

Язык: Английский

Процитировано

0
Identification and Chemical Control of Stem Canker Pathogen of Idesia polycarpa DOI Creative Commons
Jian Feng, Qiupeng Yuan,

X. Chen

и другие.

Plants, Год журнала: 2025, Номер 14(9), С. 1393 - 1393

Опубликована: Май 5, 2025

Idesia polycarpa is an important woody oilseed tree crucial for ensuring China’s grain and oil security. The expansion of I. plantations has been accompanied by increase in pests diseases, with canker disease recently observed two forests Henan Province. Field surveys revealed a incidence 70.12% among 328 surveyed trees, indicating substantial threat to plantation health. most virulent pathogen, strain SQ5, was identified as Botryosphaeria dothidea through molecular sequencing morphological analyses. Strain SQ5 showed optimum growth temperature 25 °C mycelial lethal 60 °C. pathogen thrives acidic conditions promoted light, the ability utilize various carbon nitrogen sources. In vitro toxicity assessments four effective fungicides: 70% thiophanate-methyl (EC50 = 0.0169 µg/mL), 43% tebuconazole 0.0219 20% octylamine acetate 0.0271 40% difenoconazole 0.0954 µg/mL). trials demonstrated that (average efficacy 35.29%) 23.53%) exhibited superior control canker. This study represents first systematic analysis its measures, laying foundation further research field management strategies. Given significance Chinese forestry, this underscores need strategies sustain productivity mitigate risks associated expanding plantations.

Язык: Английский

Процитировано

0
Metabolome and (R)-Mellein Production in Neofusicoccum parvum Strains are Differentially Affected by Grapevine Woody Biomass DOI
Julián D. Restrepo-Leal, Florence Fontaine, Simon Rémy

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Май 26, 2025

Neofusicoccum parvum is a wood-colonizing fungus that threatens grape production. Its ability to colonize the host may involve secretion of effector molecules, such as phytotoxic metabolite (R)-mellein. However, role (R)-mellein in disease development and factors influencing its production are poorly understood. Additionally, how this pathogen alters metabolome during interaction remains unclear. Here, we compared metabolomes two wild-type N. strains (Bt67 NpB) UV mutant (UV9) cultured with grapevine wood bioreactors. We found reduced but increased diversity secreted metabolites, whereas showed minimal changes when grown tissue. In particular, promoted auxin-related compounds, tyramines, phenylethanoids. Our findings suggest adapts profile response biomass, producing compounds antibiotic properties. Finally, was purified using Centrifugal Partition Chromatography preparative HPLC, which will aid future research into disease.

Язык: Английский

Процитировано

0
Physiological and developmental disturbances caused by Botryosphaeria dieback in the annual stems of grapevine DOI Creative Commons

Florian Moret,

Lucile Jacquens,

Philippe Larignon

и другие.

Frontiers in Plant Science, Год журнала: 2024, Номер 15

Опубликована: Апрель 23, 2024

Botryosphaeria dieback is a grapevine trunk disease caused by fungi of the Botryosphaeriaceae family, which attacks more specifically woody tissues. The infection leads to different symptoms including severe form with leaf drop as well premature plant death. causes major economic losses, since no effective treatment yet available. A better understanding necessary find solutions fight this disease. In study, our objective was characterize “leaf drop” (1) looking for presence pathogens in basal internodes stems, (2) quantifying blocked vessels tylosis and/or gummosis, and (3) describing impact on vine physiology (gene expression metabolome) development (establishment functioning cambium phellogen) at level xylem phloem stem internodes. Our study has shown that were present both annual stem, obturated. We have also clearly demonstrated gene metabolite profiles strongly modified diseased plants. Differences stems between healthy (control, C) (D) plants low flowering (vines not symptomatic), higher onset symptom still present, although less marked, full expression. qRT-PCR analysis showed an overexpression genes involved defense, repression related meristematic activity (i.e. vascular phellogen). Metabolomic specific fingerprints from expression, increase phytoalexins mannitol, decrease 1-kestose one. At structural level, many alterations observed internodes, even before symptoms: classical obstruction and, first time, disorganization secondary sieve plates callose. modifies (liber) phellogen. Altogether, combining approaches allowed highlight deep dysfunction base may explain decline due dieback.

Язык: Английский

Процитировано

1