Phytopathology Research,
Год журнала:
2025,
Номер
7(1)
Опубликована: Апрель 22, 2025
Abstract
Chestnut
blight,
caused
by
Cryphonectria
parasitica
,
is
a
notorious
disease
that
severely
harms
chestnut
species,
leading
to
great
economic
and
ecological
loss
worldwide.
Understanding
the
pathogenic
mechanisms
of
C.
crucial
for
developing
effective
control
strategies.
Therefore,
screening
functional
characterization
key
factors
during
interaction
are
essential.
Although
previous
studies
have
revealed
many
important
virulence
in
underlying
need
further
study.
Here,
we
examined
expression
pattern
at
0,
1,
2,
3
days
post
inoculation
branches
(dpi)
using
transcriptomic
sequencing.
A
total
2160
differentially
expressed
genes
(DEGs)
were
identified
infection
stages
compared
uninfected
0
dpi.
Gene
ontology
(GO)
Kyoto
encyclopedia
genomes
(KEGG)
analyses
showed
significant
enrichment
upregulated
glycoside
hydrolase
activity
carbohydrate
metabolism
pathways.
Clustering
analysis
66
significantly
with
higher
fold
changes
process,
most
which
related
degradation
host
cell
wall.
Additionally,
48
putative
effector
induced
stages,
annotated
as
hydrolases
chitinases
well
hydrophobic
proteins,
indicating
roles
processes.
Subsequently,
preliminary
assayed
gene
CpEng1
found
it
was
essential
fungal
virulence.
The
results
presented
here
provide
resource
understanding
molecular
basis
between
its
host.
