Spatial transcriptomics analysis identifies therapeutic targets in diffuse high-grade gliomas

Frontiers in Molecular Neuroscience, Год журнала: 2024, Номер 17

Опубликована: Окт. 24, 2024

Introduction Diffuse high-grade gliomas are the most common malignant adult neuroepithelial tumors in humans and a leading cause of cancer-related death worldwide. The advancement high throughput transcriptome sequencing technology enables rapid comprehensive acquisition data from target cells or tissues. This aids researchers understanding identifying critical therapeutic targets for prognosis treatment diffuse glioma. Methods Spatial transcriptomics was conducted on two cases isocitrate dehydrogenase (IDH) wild-type glioma (Glio-IDH-wt) IDH-mutant (Glio-IDH-mut). Gene set enrichment analysis clustering were employed to pinpoint differentially expressed genes (DEGs) involved progression gliomas. spatial distribution DEGs spatially defined regions human tissues overlaid t-distributed stochastic neighbor embedding (t-SNE) plots. Results We identified total 10,693 DEGs, with 5,677 upregulated 5,016 downregulated, Specifically, SPP1 , IGFBP2 CALD1 TMSB4X exhibited expression carcinoma both Glio-IDH-wt Glio-IDH-mut, 3 ( SMOC1 APOE HIPK2 ) 4 PPP1CB UBA52 S100A6 CTSB only tumor respectively. Moreover, Kyoto Encyclopedia Genes Genomes (KEGG) gene ontology (GO) analyses revealed that closely related PI3K/Akt signaling pathway, virus infection, cytokine-cytokine receptor interaction. Importantly, these validated using GEPIA databases. Furthermore, study patterns key regulatory genes, including those protein post-translational modification RNA binding protein-encoding Discussion is one breakthroughs field medical biotechnology as this can map analytes such information their physical location tissue sections. Our findings illuminate previously unexplored profiles biomarkers glioma, offering novel insight development strategies

Язык: Английский

Prognostic signature and immunotherapeutic relevance of Focal adhesion signaling pathway-related genes in osteosarcoma

Heliyon, Год журнала: 2024, Номер 10(21), С. e38523 - e38523

Опубликована: Сен. 26, 2024

Язык: Английский