Study on the changes of extracellular matrix morphology and components in COPD animal model by using lung decellularized scaffold DOI Creative Commons
Yuan Li,

Y.-M. Dai,

Ting Jin

и другие.

The FASEB Journal, Год журнала: 2025, Номер 39(6)

Опубликована: Март 27, 2025

Abstract Airway remodeling is a critical pathological process that influences the progression of chronic obstructive pulmonary disease(COPD). To better study small airway in COPD, we employed advanced techniques such as decellularized scaffolds, immunofluorescence, scanning electron microscopy, and proteomics to analyze morphological compositional changes extracellular matrix (ECM). Our revealed significant ultrastructural abnormalities scaffolds from COPD group, including thinning alveolar septa, enlargement spaces, fusion multiple alveoli. Additionally, ECM composition group exhibited notable characterized by an increase collagen fibers, type I IV collagens, fibronectin, laminin ( p < .05), along with decrease elastin glycosaminoglycans .05). Proteomic analysis identified 70 differentially expressed proteins between control group. These included 34 upregulated Smarca2, Skt, Acvrl1, Myl2 (all ratios >10.64), 36 downregulated Col6a6, Col6a5, AnK3 <0.27). Pathway indicated activation apoptosis (Enrichment Score, ES = 0.23) epithelial–mesenchymal transition (ES 0.38) genes inhibition synthesis –0.43) degradation –0.63) were observed findings enhance our understanding mechanisms underlying provide scientific basis for developing novel therapeutic strategies COPD.

Язык: Английский

Study on the changes of extracellular matrix morphology and components in COPD animal model by using lung decellularized scaffold DOI Creative Commons
Yuan Li,

Y.-M. Dai,

Ting Jin

и другие.

The FASEB Journal, Год журнала: 2025, Номер 39(6)

Опубликована: Март 27, 2025

Abstract Airway remodeling is a critical pathological process that influences the progression of chronic obstructive pulmonary disease(COPD). To better study small airway in COPD, we employed advanced techniques such as decellularized scaffolds, immunofluorescence, scanning electron microscopy, and proteomics to analyze morphological compositional changes extracellular matrix (ECM). Our revealed significant ultrastructural abnormalities scaffolds from COPD group, including thinning alveolar septa, enlargement spaces, fusion multiple alveoli. Additionally, ECM composition group exhibited notable characterized by an increase collagen fibers, type I IV collagens, fibronectin, laminin ( p < .05), along with decrease elastin glycosaminoglycans .05). Proteomic analysis identified 70 differentially expressed proteins between control group. These included 34 upregulated Smarca2, Skt, Acvrl1, Myl2 (all ratios >10.64), 36 downregulated Col6a6, Col6a5, AnK3 <0.27). Pathway indicated activation apoptosis (Enrichment Score, ES = 0.23) epithelial–mesenchymal transition (ES 0.38) genes inhibition synthesis –0.43) degradation –0.63) were observed findings enhance our understanding mechanisms underlying provide scientific basis for developing novel therapeutic strategies COPD.

Язык: Английский

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