An Update on Animal Models of Alcohol-Associated Liver Disease

American Journal Of Pathology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

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A novel mitochondrial quality regulation gene signature for anticipating prognosis, TME, and therapeutic response in LUAD by multi-omics analysis and experimental verification

Cancer Cell International, Год журнала: 2025, Номер 25(1)

Опубликована: Апрель 10, 2025

Lung adenocarcinoma (LUAD) is the predominant form of non-small cell lung cancer (NSCLC). Mitochondrial quality-related genes (MQRGs) contribute to genesis and advancement tumors. Despite advances in LUAD treatment detection, early diagnostic biomarkers are still lacking, roles MQRGs not well understood. We extensively examined transcriptome clinical data from TCGA GEO databases discover differentially expressed MQRGs. Utilizing LASSO algorithm multivariate COX regression, a predictive risk model was created. Kaplan-Meier study ROC curves were implemented predict patient prognosis, resulting new Quality Regulation Gene Signature for accurate prognosis forecasting. R software packages facilitated statistical, consensus cluster, survival, Cox Lasso tumor microenvironment analyses. Model-related gene expression measured using RT-qPCR, immunohistochemistry, single-cell sequencing, HPA data, UNCAN data. created concise four (STRAP, SHCBP1, PKP2, CRTAC1) forecast overall survival patients. High-risk patients experienced significantly lower rates. Functional analysis linked these alpha-linolenic acid metabolism pathways. Moreover, immune supports previous findings that higher CD8 + T infiltration improves outcomes. Analysis different scores showed increased activated memory T-cell CD4, suggesting its activation crucial prognosis. Nomograms generated with MQRGscore model. mRNA IHC manifested upregulated STRAP, PKP2 mitigated CRTAC1 contrasted normal tissue. qRT-PCR immunohistochemistry confirmed findings, aligning succinct ascertain patient's potentially offering novel method diagnosing treating this condition.

Язык: Английский

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Naringin prevents the impairment of hepatic mitochondrial function in diabetic rats

Canadian Journal of Physiology and Pharmacology, Год журнала: 2025, Номер unknown

Опубликована: Апрель 23, 2025

We previously demonstrated that naringin (NAR) protects against liver damage in streptozotocin (STZ)-induced diabetes rats. The aim of this study was to elucidate whether NAR is also able protect the functioning, biogenesis and dynamics mitochondria diabetic rats (DM). activities isocitrate dehydrogenase malate from Krebs cycle, complex I–III electron chain adenosine triphosphate synthase were decreased DM rats, effects blocked by NAR. gene expression mitofusin-2 GTPase dynamin-related protein 1, markers mitochondrial fusion fission, which prevented Total glutathione carbonyl contents as well activity antioxidant enzymes increased All these changes In conclusion, avoiding respiratory oxidative phosphorylation preventing alterations fusion-fission processes. These are mediated, at least part, decreasing stress anomalies enzymatic system. Further studies necessary validate efficacy safety for human use.

Язык: Английский

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Paternal Mitochondrial DNA Leakage in Natural Populations of Large-Scale Loach, Paramisgurnus dabryanus

Biology, Год журнала: 2024, Номер 13(8), С. 604 - 604

Опубликована: Авг. 10, 2024

Animal mitochondrial DNA is usually considered to comply with strict maternal inheritance, and only one haplotype exists in an individual. However, heteroplasmy, the occurrence of more than haplotype, has recently been reported some animals, such as mice, mussels, birds. This study conducted extensive field surveys obtain representative samples investigate existence paternal inheritance (mtDNA) natural fish populations. Evidence leakage P. dabryanus was discovered using high-throughput sequencing bioinformatics methods. Two distinct haplotypes (16,569 bp for I 16,646 II) were observed, differing by 18.83% nucleotide sequence. Phylogenetic analysis suggests divergence between these potential interspecific hybridization M. anguillicaudatus, leading leakage. In populations along Yangtze River, both are present, Type being dominant (75% copy number). Expression shows that higher expression levels ND3 ND6 genes compared II, suggesting I’s primary role. discovery a species two types provides model studying heterogeneity insights into genome evolution inheritance.

Язык: Английский

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Resveratrol promotes mitophagy via the MALAT1/miR-143-3p/RRM2 axis and suppresses cancer progression in hepatocellular carcinoma

Journal of Integrative Medicine, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 1, 2024

Язык: Английский

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