The Next Chapter in Cancer Diagnostics: Advances in HPV-Positive Head and Neck Cancer

Biomolecules, Год журнала: 2024, Номер 14(8), С. 925 - 925

Опубликована: Июль 30, 2024

Human papillomavirus (HPV)-associated head and neck squamous cell carcinoma (HNSCC), particularly oropharyngeal (OPSCC), is an increasingly prevalent pathology worldwide, especially in developed countries. For diagnosing HPV HNSCC, the combination of p16 immunohistochemistry (IHC) polymerase chain reaction (PCR) offers high sensitivity specificity, with IHC being a reliable initial screen PCR confirming presence. Advanced techniques like next-generation sequencing (NGS) RNA-based assays provide detailed insights but are primarily used research settings. Regardless status, standard oncological treatments currently include surgery, radiation, and/or chemotherapy. This conventional approach does not account for typically better prognosis HPV-positive HNSCC patients, leading to increased chemo/radiation-induced secondary morbidities reduced quality life. Therefore, it crucial identify detect positivity other molecular characteristics personalize treatment strategies. comprehensive review aims summarize current knowledge on various detection evaluate their advantages disadvantages, focus developing methodologies new biomarkers HNSCC. The discusses direct indirect examination tumor tissue, DNA- techniques, protein-based markers, liquid biopsy potentials, immune-related epigenetic novel biomarkers, emerging technologies, providing overall insight into state knowledge.

Язык: Английский

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Accuracy of liquid-based brush cytology and HPV detection for the diagnosis and management of patients with oropharyngeal and oral cancer

Clinical Oral Investigations, Год журнала: 2021, Номер 26(3), С. 2587 - 2595

Опубликована: Ноя. 27, 2021

This study aims to evaluate the usefulness of liquid-based brush cytology for malignancy diagnosis and HPV detection in patients with suspected oropharyngeal oral carcinomas, as well tumoral persistence after treatment.Seventy-five suspicion squamous cell carcinoma oropharynx or cavity were included. Two different groups analyzed according date sample collection: (1) during first endoscopy exploration (2) control treatment carcinoma. Sensitivity, specificity, positive predictive value, negative accuracy HPV-DNA on cytologies assessed.Before treatment, showed a sensitivity 88%, specificity 100%, 88%. After it 71%, 77%, 75%. samples 85%, 91% before an 100% treatment.Liquid-based good but its value decreases treatment. Nevertheless, is useful detection, monitor treatment.Brush are reliable may be method incorporate testing guidelines.

Язык: Английский

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The Pathology according to p53 Pathway

Pathobiology, Год журнала: 2023, Номер 91(3), С. 230 - 244

Опубликована: Ноя. 14, 2023

Observations play a pivotal role in the progress of science, including pathology. The cause disease such as cancer is analyzed by breaking it down into smaller organs, tissues, cells, and molecules. current standard diagnostic procedure, microscopic observation, relies on preserved morphological characteristics. In contrast, molecular analyses explore oncogenic pathway activation that leads to genetic mutations aberrant protein expression. Such could potentially identify therapeutic targets has gained considerable attention clinical oncology.

Язык: Английский

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A simplified molecular method to detect high‐risk HPV using the Aptima HPV assay on head and neck FNA smears

Cancer Cytopathology, Год журнала: 2022, Номер 131(3), С. 171 - 178

Опубликована: Окт. 26, 2022

Fine-needle aspiration is used as a diagnostic tool in head and neck oropharyngeal squamous cell carcinoma its metastases. Prognosis treatment rely on the presence or absence of human papilloma virus. The purpose this study was to validate performance Aptima HPV assay using Hema-Diff stained fine-needle smears diagnosis virus-related simplified method obtain tumor cells for testing.Patients with positive p16 immunohistochemical staining were identified. Specimen Transport Media remove from slides moistened swab. selected tested high risk-human virus 16 18/45 genotype assay. results compared related block surgical specimens.Twenty-one 21 (100%) p16-positive cases found be virus, whereas 20 (95%) negative assay.The can detect high-risk sensitivity 100% specificity 95%. This provides valuable alternative sections that often lack appropriate numbers cells.

Язык: Английский

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A Case of HPV-Associated Oropharyngeal Squamous Cell Carcinoma with Block-Like, Partial Loss of p16 Expression

Head and Neck Pathology, Год журнала: 2022, Номер 16(4), С. 1251 - 1256

Опубликована: Июнь 30, 2022

Язык: Английский

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