Cytoskeletal crosstalk: A focus on intermediate filaments
Current Opinion in Cell Biology,
Год журнала:
2024,
Номер
87, С. 102325 - 102325
Опубликована: Фев. 14, 2024
The
cytoskeleton,
comprising
actin
microfilaments,
microtubules,
and
intermediate
filaments,
is
crucial
for
cell
motility
tissue
integrity.
While
prior
studies
largely
focused
on
individual
cytoskeletal
networks,
recent
research
underscores
the
interconnected
nature
of
these
systems
in
fundamental
cellular
functions
like
adhesion,
migration,
division.
Understanding
coordination
distinct
networks
both
time
space
essential.
This
review
synthesizes
current
findings
intricate
interplay
between
emphasizing
pivotal
role
filaments.
Notably,
filaments
engage
extensive
crosstalk
with
microfilaments
microtubules
through
direct
molecular
interactions,
linkers,
motors
that
form
bridges,
as
well
via
more
complex
regulation
intracellular
signaling.
Язык: Английский
Vimentin supports cell polarization by enhancing centrosome function and microtubule acetylation
Journal of The Royal Society Interface,
Год журнала:
2024,
Номер
21(215)
Опубликована: Июнь 1, 2024
Cell
polarity
is
important
for
controlling
cell
shape,
motility
and
division
processes.
Vimentin
intermediate
filaments
are
migration
polarization
in
mesenchymal
cells
assembly
of
vimentin
microtubule
networks
dynamically
coordinated,
but
the
precise
details
how
mediates
remain
unclear.
Here,
we
characterize
effects
on
structure
function
centrosome
stability
wild-type
vimentin-null
mouse
embryonic
fibroblasts.
We
find
that
pericentriolar
material,
promotes
centrosome-mediated
regrowth
increases
level
stable
acetylated
microtubules
cell.
Loss
also
impairs
repositioning
during
processes
occur
wound
closure.
Our
results
suggest
modulates
as
well
network
stability,
which
has
implications
establish
proper
persistent
migration.
Язык: Английский
Endothelial cytoskeleton in mechanotransduction and vascular diseases
Journal of Biomechanics,
Год журнала:
2025,
Номер
182, С. 112579 - 112579
Опубликована: Фев. 9, 2025
Язык: Английский
Vimentin promotes collective cell migration through collagen networks via increased matrix remodeling and spheroid fluidity
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Июнь 17, 2024
The
intermediate
filament
(IF)
protein
vimentin
is
associated
with
many
diseases
phenotypes
of
enhanced
cellular
migration
and
aggressive
invasion
through
the
extracellular
matrix
(ECM)
tissues,
but
vimentin's
role
in
in-vivo
cell
still
largely
unclear.
Vimentin
important
for
proper
adhesion
force
generation,
which
are
critical
to
migration;
yet
cytoskeleton
also
hinders
ability
cells
squeeze
small
pores
ECM,
resisting
migration.
To
identify
collective
migration,
we
generate
spheroids
wide-type
vimentin-null
mouse
embryonic
fibroblasts
(mEFs)
embed
them
a
3D
collagen
matrix.
We
find
that
loss
significantly
impairs
spheroid
collectively
expand
networks
remodel
network.
Traction
analysis
reveals
null
exert
less
contractile
than
their
wild-type
counterparts.
In
addition,
made
mEFs
only
unit
length
filaments
(ULFs)
exhibit
similar
behavior
as
spheroids,
suggesting
filamentous
required
promote
vimentin-mediated
expansion
dependent
on
metalloproteinase
(MMP)
degradation
Further,
vertex
model
simulation
embedded
ECM
indicates
behave
more
fluid-like,
enabling
active
pulling
reconstructing
surrounding
Altogether,
these
results
signify
VIF
plays
enhancing
migratory
persistence
environments
MMP
transportation
tissue
fluidity.
Язык: Английский
LINC01559: roles, mechanisms, and clinical implications in human cancers
Human Cell,
Год журнала:
2025,
Номер
38(3)
Опубликована: Апрель 9, 2025
Язык: Английский
Vimentin – Force regulator in confined environments
Current Opinion in Cell Biology,
Год журнала:
2025,
Номер
94, С. 102521 - 102521
Опубликована: Апрель 26, 2025
Cells
must
navigate
crowded
and
confining
3D
environments
during
normal
function
in
vivo.
Essential
to
their
ability
these
safely
efficiently
is
mediate
endure
both
self-generated
external
forces.
The
cytoskeleton,
composed
of
F-actin,
microtubules,
intermediate
filaments,
provides
the
mechanical
support
necessary
for
force
mediation.
role
F-actin
microtubules
this
process
has
been
well
studied,
whereas
vimentin,
a
cytoplasmic
filament
associated
with
mesenchymal
cells,
less
studied.
However,
there
growing
evidence
that
vimentin
functions
transmission
protection
cell
from
stress
actin
cannot
fulfill.
This
review
focuses
on
recent
reports
highlighting
vimentin's
regulating
forces
environments.
Язык: Английский
Global alignment and local curvature of microtubules in mouse fibroblasts are robust against perturbations of vimentin and actin
Soft Matter,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 23, 2024
The
analysis
of
fluorescence
microscopy
images
micropatterned
mouse
fibroblasts
reveals
a
robust
radial
organization
microtubules.
Furthermore,
on
average,
microtubule
curvatures
are
independent
actin
and
vimentin.
Язык: Английский
Vimentin intermediate filaments structure and mechanically support microtubules in cells
Anna Blob,
David Ventzke,
Giacomo Nies
и другие.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Апрель 21, 2023
Abstract
The
eukaryotic
cytoskeleton
is
an
intricate
network
of
three
types
mechanically
distinct
biopolymers
–
actin
filaments,
microtubules
and
intermediate
filaments
(IFs).
These
filamentous
networks
determine
essential
cellular
functions
properties.
Among
them,
are
important
for
intracellular
transport
establishing
cell
polarity
during
migration.
Despite
their
intrinsic
stiffness,
they
exhibit
characteristic
bending
buckling
in
cells
due
to
non
thermal
forces
acting
on
them.
Interactions
between
cytoskeletal
have
been
found
but
complex
diverse
with
respect
effect
the
mechanical
behavior
architecture
networks.
We
systematically
study
how
vimentin
IFs
influence
structure
local
by
analyzing
fluorescence
microscopy
images
mouse
fibroblasts
protein
micropatterns.
Our
automated
analysis
averages
over
large
amounts
data
mitigate
considerable
natural
variance
biological
data.
find
that
radial
orientation
circular
robust
established
independently
Observing
curvature
microtubules,
we
highly
similar
average
entire
regardless
surrounding.
Small
systematic
differences
cannot
be
attributed
directly
densities.
results
suggest
that,
average,
unpolarized
unexpectedly
independent
rest
global
response
forces.
Язык: Английский