Vimentin intermediate filaments structure and mechanically support microtubules in cells DOI Creative Commons

Anna Blob,

David Ventzke,

Giacomo Nies

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Апрель 21, 2023

Abstract The eukaryotic cytoskeleton is an intricate network of three types mechanically distinct biopolymers – actin filaments, microtubules and intermediate filaments (IFs). These filamentous networks determine essential cellular functions properties. Among them, are important for intracellular transport establishing cell polarity during migration. Despite their intrinsic stiffness, they exhibit characteristic bending buckling in cells due to non thermal forces acting on them. Interactions between cytoskeletal have been found but complex diverse with respect effect the mechanical behavior architecture networks. We systematically study how vimentin IFs influence structure local by analyzing fluorescence microscopy images mouse fibroblasts protein micropatterns. Our automated analysis averages over large amounts data mitigate considerable natural variance biological data. find that radial orientation circular robust established independently Observing curvature microtubules, we highly similar average entire regardless surrounding. Small systematic differences cannot be attributed directly densities. results suggest that, average, unpolarized unexpectedly independent rest global response forces.

Язык: Английский

Cytoskeletal crosstalk: A focus on intermediate filaments DOI Creative Commons
Lucas Pradeau-Phélut, Sandrine Étienne-Manneville

Current Opinion in Cell Biology, Год журнала: 2024, Номер 87, С. 102325 - 102325

Опубликована: Фев. 14, 2024

The cytoskeleton, comprising actin microfilaments, microtubules, and intermediate filaments, is crucial for cell motility tissue integrity. While prior studies largely focused on individual cytoskeletal networks, recent research underscores the interconnected nature of these systems in fundamental cellular functions like adhesion, migration, division. Understanding coordination distinct networks both time space essential. This review synthesizes current findings intricate interplay between emphasizing pivotal role filaments. Notably, filaments engage extensive crosstalk with microfilaments microtubules through direct molecular interactions, linkers, motors that form bridges, as well via more complex regulation intracellular signaling.

Язык: Английский

Процитировано

12

Vimentin supports cell polarization by enhancing centrosome function and microtubule acetylation DOI Creative Commons
Renita Saldanha,

Minh Tri Ho Thanh,

Nikhila Krishnan

и другие.

Journal of The Royal Society Interface, Год журнала: 2024, Номер 21(215)

Опубликована: Июнь 1, 2024

Cell polarity is important for controlling cell shape, motility and division processes. Vimentin intermediate filaments are migration polarization in mesenchymal cells assembly of vimentin microtubule networks dynamically coordinated, but the precise details how mediates remain unclear. Here, we characterize effects on structure function centrosome stability wild-type vimentin-null mouse embryonic fibroblasts. We find that pericentriolar material, promotes centrosome-mediated regrowth increases level stable acetylated microtubules cell. Loss also impairs repositioning during processes occur wound closure. Our results suggest modulates as well network stability, which has implications establish proper persistent migration.

Язык: Английский

Процитировано

8

Endothelial cytoskeleton in mechanotransduction and vascular diseases DOI

Linlu Jin,

Yixue Qin,

Yili Zhao

и другие.

Journal of Biomechanics, Год журнала: 2025, Номер 182, С. 112579 - 112579

Опубликована: Фев. 9, 2025

Язык: Английский

Процитировано

1

Vimentin promotes collective cell migration through collagen networks via increased matrix remodeling and spheroid fluidity DOI Creative Commons

Minh Tri Ho Thanh,

Arun Poudel,

Shabeeb Ameen

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 17, 2024

The intermediate filament (IF) protein vimentin is associated with many diseases phenotypes of enhanced cellular migration and aggressive invasion through the extracellular matrix (ECM) tissues, but vimentin's role in in-vivo cell still largely unclear. Vimentin important for proper adhesion force generation, which are critical to migration; yet cytoskeleton also hinders ability cells squeeze small pores ECM, resisting migration. To identify collective migration, we generate spheroids wide-type vimentin-null mouse embryonic fibroblasts (mEFs) embed them a 3D collagen matrix. We find that loss significantly impairs spheroid collectively expand networks remodel network. Traction analysis reveals null exert less contractile than their wild-type counterparts. In addition, made mEFs only unit length filaments (ULFs) exhibit similar behavior as spheroids, suggesting filamentous required promote vimentin-mediated expansion dependent on metalloproteinase (MMP) degradation Further, vertex model simulation embedded ECM indicates behave more fluid-like, enabling active pulling reconstructing surrounding Altogether, these results signify VIF plays enhancing migratory persistence environments MMP transportation tissue fluidity.

Язык: Английский

Процитировано

3

LINC01559: roles, mechanisms, and clinical implications in human cancers DOI
Shuwen Zhang, Xin Lan, Lei Liu

и другие.

Human Cell, Год журнала: 2025, Номер 38(3)

Опубликована: Апрель 9, 2025

Язык: Английский

Процитировано

0

Vimentin – Force regulator in confined environments DOI Creative Commons
Maxx Swoger,

Minh Tri Ho Thanh,

Alison E. Patteson

и другие.

Current Opinion in Cell Biology, Год журнала: 2025, Номер 94, С. 102521 - 102521

Опубликована: Апрель 26, 2025

Cells must navigate crowded and confining 3D environments during normal function in vivo. Essential to their ability these safely efficiently is mediate endure both self-generated external forces. The cytoskeleton, composed of F-actin, microtubules, intermediate filaments, provides the mechanical support necessary for force mediation. role F-actin microtubules this process has been well studied, whereas vimentin, a cytoplasmic filament associated with mesenchymal cells, less studied. However, there growing evidence that vimentin functions transmission protection cell from stress actin cannot fulfill. This review focuses on recent reports highlighting vimentin's regulating forces environments.

Язык: Английский

Процитировано

0

Global alignment and local curvature of microtubules in mouse fibroblasts are robust against perturbations of vimentin and actin DOI Creative Commons

Anna Blob,

David Ventzke,

Ulrike Rölleke

и другие.

Soft Matter, Год журнала: 2024, Номер unknown

Опубликована: Дек. 23, 2024

The analysis of fluorescence microscopy images micropatterned mouse fibroblasts reveals a robust radial organization microtubules. Furthermore, on average, microtubule curvatures are independent actin and vimentin.

Язык: Английский

Процитировано

1

Vimentin intermediate filaments structure and mechanically support microtubules in cells DOI Creative Commons

Anna Blob,

David Ventzke,

Giacomo Nies

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Апрель 21, 2023

Abstract The eukaryotic cytoskeleton is an intricate network of three types mechanically distinct biopolymers – actin filaments, microtubules and intermediate filaments (IFs). These filamentous networks determine essential cellular functions properties. Among them, are important for intracellular transport establishing cell polarity during migration. Despite their intrinsic stiffness, they exhibit characteristic bending buckling in cells due to non thermal forces acting on them. Interactions between cytoskeletal have been found but complex diverse with respect effect the mechanical behavior architecture networks. We systematically study how vimentin IFs influence structure local by analyzing fluorescence microscopy images mouse fibroblasts protein micropatterns. Our automated analysis averages over large amounts data mitigate considerable natural variance biological data. find that radial orientation circular robust established independently Observing curvature microtubules, we highly similar average entire regardless surrounding. Small systematic differences cannot be attributed directly densities. results suggest that, average, unpolarized unexpectedly independent rest global response forces.

Язык: Английский

Процитировано

1