First Report of a ‘Candidatus Phytoplasma australasiaticum’-Related Phytoplasma Strain Associated with Shoot Proliferation Disease of Variegated Croton in Taiwan DOI

Reun‐Ping Goh,

S. Lee,

Chia‐Ching Chu

и другие.

Plant Disease, Год журнала: 2023, Номер 108(3), С. 781 - 781

Опубликована: Дек. 21, 2023

Codiaeum variegatum (family Euphorbiaceae) is a leaf ornamental commonly known as variegated croton, which often found in gardens or grown indoor plants. In December of 2022, two cutting-propagated croton plants exhibiting abnormal shoot proliferation and little symptoms (Fig. S1) were nursery owned by private breeder Wanluan Township, Pingtung County, Taiwan. The propagated from single stock plant, died during transplanting commercial Changzhi County. To determine the potential cause such symptoms, tissues collected center symptomatic Their DNA extracted with Synergy 2.0 Plant Extraction Kit (OPS Diagnostics, Lebanon, NJ) used for further testing. As controls, symptomless Township to produce plants; was each plant described above. samples subjected PCR testing using phytoplasma-specific primer pair P1/P7 (Schneider et al. 1995), only produced expected 1.8-kb amplicon. phytoplasma isolates detected designated CvaA CvaB. After sequencing analyzing data iPhyClassifier program (Zhao 2009), both CvaB classified subgroup 16SrII-A (GenBank accession no. L33765) similarity coefficient 1.0. near-full-length 16S rDNA fragments OR794242) also identical (1,463/1,463 bp) that NCHU2014 CP040925, bp 537768-539230), reference 'Ca. Phytoplasma australasiaticum'-related strain (16SrII-A) Taiwan (Chang 2015; Rodrigues Jardim 2023). validate results, tested 16SrII group-specific semi-nested targeting elongation factor Tu gene. outer inner pairs TUF-II-F1/TUF-II-R1 TUF-II-F2/TUF-II-R1, respectively (Al-Subhi 2017). An amplicon but not counterparts. amplified fragments' sequences OR634931) gene (989/989 bp; GenBank 139344-140332). protein translocase secY sequenced primers SecYF1(II), SecYF2(II) SecYR1(II) (Lee 2010). Again, OR862773) (1,263/1,263; 192846-194108). quality confirmed host's 28S 28KJ/28C (Cullings 1992) all target (0.7 kb). phytoplasmas have been different host 2015). our knowledge, this first record group pathogens infecting Branch-inducing has improve values poinsettias, another Euphorbiaceae species 1997). Further needed whether work could be similar purposes.

Язык: Английский

Strawberry Green Petal Disease Pathosystem in Québec, Canada DOI Creative Commons
Abraão Almeida Santos,

Joshua Molligan,

Nicolas Plante

и другие.

Plant Health Cases, Год журнала: 2025, Номер unknown

Опубликована: Фев. 17, 2025

Abstract The emergence and re-emergence of pathosystems are expected to increase with ongoing climate change. Strawberry green petal disease (SbGP) is caused by phytoplasmas transmitted two leafhopper species, Aphrodes bicinctus Macrosteles quadrilineatus , in strawberry crops. In Québec, SbGP was frequently reported between 1950 1970. However, recent surveys (2012–2022) indicate an increased incidence fields. While it unclear whether this indicates a or underreporting, understanding these pathosystem components can help clarify the underlying causes. This case study reviews pathosystem, historical reports, existing control measures, emphasizing importance future research address identified gaps. Although currently poses limited economic threat, interactions within could be exacerbated warming temperatures, potentially leading vector populations, altered phytoplasma development, susceptibility plants, consequently, severe outbreaks broader geographic distribution disease. Information © Authors 2025

Язык: Английский

Процитировано

0
Distribution and characterization of phytoplasmas infecting flower crops with diverse symptoms in Andhra Pradesh State, India DOI
D. Meghanath,

K. Arunodhayam,

Madem Gurivi Reddy

и другие.

Australasian Plant Pathology, Год журнала: 2025, Номер unknown

Опубликована: Март 20, 2025

Язык: Английский

Процитировано

0
A metagenomic investigation of phytoplasma diversity in Australian vegetable growing regions DOI Creative Commons
Bianca Rodrigues Jardim, Cherie Gambley, Lucy T. T. Tran‐Nguyen

и другие.

Microbial Genomics, Год журнала: 2024, Номер 10(3)

Опубликована: Март 6, 2024

In this study, metagenomic sequence data was used to investigate the phytoplasma taxonomic diversity in vegetable-growing regions across Australia. Metagenomic sequencing performed on 195 phytoplasma-positive samples, originating either from historic collections ( n =46) or during collection efforts between January 2015 and June 2022 =149). The sampled hosts were classified as crop =155), weed =24), ornamental =7), native plant =6), insect =3) species. Most samples came Queensland =78), followed by Western Australia =46), Northern Territory =32), New South Wales =17), Victoria =10). Of draft genomes, 178 met our genome criteria for comparison using an average nucleotide identity approach. Ten distinct species identified could be within 16SrII, 16SrXII (PCR only), 16SrXXV, 16SrXXXVIII groups, which have all previously been recorded most commonly detected taxa study subspecies 16SrII group =153), strains (‘ Ca . Phytoplasma stylosanthis’; =6). Several geographic- host-range expansions reported, well mixed infections of ‘ stylosanthis’. Additionally, six unrecorded identified, including five putative australasiaticum’ a new PCR 16S rRNA gene suitable triage tool preliminary detection. sequencing, however, allowed higher-resolution identification phytoplasmas, infections, than afforded only direct Sanger gene. Since approach theoretically obtains sequences organisms sample, useful confirm host family, genus, and/or addition improving understanding that affect production Australia, also significantly expands genomic available public repositories contribute molecular epidemiology studies, revision taxonomy, improved diagnostics.

Язык: Английский

Процитировано

2
Phytoplasma-Associated Diseases in South America: Thirty Years of Research DOI Creative Commons
H. G. Montano, Assunta Bertaccini, Nicola Fiore

и другие.

Microorganisms, Год журнала: 2024, Номер 12(7), С. 1311 - 1311

Опубликована: Июнь 27, 2024

Phytoplasma-associated diseases are mainly insect-transmitted and present worldwide. Considering that disease detection is a relevant environmental factor may elucidate the presence of these diseases, review reporting geographic distribution phytoplasma taxa in geographically consistent areas helps manage appropriately reduce their spreading. This work summarizes data available about identification associated with several diverse South America last decades. The insect vectors putative together plant host range phytoplasmas also summarized. Overall, 16 '

Язык: Английский

Процитировано

2
Molecular characterization of ‘Candidatus Phytoplasma australasiaticum’ associated with ornamentals, amaranthus, french bean and sunhemp crops and development of rapid LAMP assay for detection DOI

K. V. Ashwathappa,

Mantesh Muttappagol,

V. Venkataravanappa

и другие.

Physiological and Molecular Plant Pathology, Год журнала: 2024, Номер unknown, С. 102423 - 102423

Опубликована: Сен. 1, 2024

Язык: Английский

Процитировано

2
Multilocus Gene Analyses Indicate Tamarix aphylla as Reservoir Host of Diverse Phytoplasmas Associated with Witches’ Broom and Yellowing Symptomatology DOI Creative Commons
Seyyed Alireza Esmaeilzadeh‐Hosseini, Ghobad Babaei,

Francesco Pacini

и другие.

Plants, Год журнала: 2024, Номер 13(9), С. 1248 - 1248

Опубликована: Апрель 30, 2024

Tamarisk witches’ broom, yellowing, and little leaf symptoms were observed during 2018–2023 surveys of rural deserts in central regions Iran with the highest disease incidence up to 72% Chah Afzal (Yazd province). A verification presence identity phytoplasmas associated these was then performed. tree branch cuttings obtained from symptomatic plants sprouted 90.3% but 15–25 days’ delay compared asymptomatic ones showed internode shortening while had normal growth 97.8%. Phytoplasma transmission by dodder bridges periwinkle did not succeed, nested polymerase chain reaction on phytoplasma ribosomal gene followed RFLP phylogenetic analyses revealed ‘Candidatus asteris’, ‘Ca. P. australasiae=australasiaticum’, trifolii’ (ribosomal subgroups 16SrI-B, 16SrII-D, 16SrVI-A, respectively) samples only. Further amplifications performed selected phytoplasma-positive tuf secA genes, produced sequences indicated mixed infection some samples. In particular, gene, a australasiae=australasiaticum’ detected, asteris’ or tritici’ strains identified. The first-time detection diverse T. aphylla suggests that this species represent relevant source for agricultural crops landscape especially when temperature allows insect vector transmission, therefore, it represents risk every environment frame climatic changes.

Язык: Английский

Процитировано

1
Detection and molecular characterization of ‘Candidatus Phytoplasma australasiaticum’ in Aegle marmelos: a fruit of high medicinal values in India DOI
Sushil Kumar Singh, Hemavati Ranebennur,

Shivani Gupta

и другие.

3 Biotech, Год журнала: 2024, Номер 14(5)

Опубликована: Май 1, 2024

Язык: Английский

Процитировано

1
Utilization of 16Sr RNA and secA genes for molecular discernment of ‘Candidatus Phytoplasma australasiaticum’ strain associated with linseed germplasm in India DOI
Shashank Kumar Yadav,

Devender Singh,

Lakshman Prasad

и другие.

3 Biotech, Год журнала: 2024, Номер 14(9)

Опубликована: Авг. 13, 2024

Язык: Английский

Процитировано

1
Complete genome sequence of Candidatus Phytoplasma australasiaticum WF_GM2021, a plant pathogen associated with soybean witches’ broom disease in Taiwan DOI Open Access

Nian-Pu Li,

Xiao-Hua Yan, Shen-Chian Pei

и другие.

Microbiology Resource Announcements, Год журнала: 2024, Номер 13(2)

Опубликована: Янв. 11, 2024

The complete genome sequence of Candidatus Phytoplasma australasiaticum strain WF_GM2021, which consists one 633,005-bp circular chromosome, is presented in this work. This uncultivated plant-pathogenic bacterium associated with soybean (Glycine max) witches' broom disease Wufeng District, Taichung City, Taiwan.

Язык: Английский

Процитировано

0
First Report of ‘Candidatus Phytoplasma citri’-Related Strain (16SrII-C Subgroup) Associated with Phyllody Disease of Cumin in India DOI
Manish Kumar, Kartar Singh,

Ramesh

и другие.

Plant Disease, Год журнала: 2024, Номер 108(4), С. 1094 - 1094

Опубликована: Фев. 21, 2024

Cumin (Cuminum cyminum L.), is an important export-oriented seed spice crop for India. popularly used flavouring food, including soups, pickles and vegetables, herbal medicine. India the largest producer, consumer exporter of cumin with annual production 0.795 million tones over area 1.09 hectares. During 2020-21, exported about 0.299 tons worth Rs 33280 (Anonymous, 2021). Recently, phytoplasma suspected symptoms were observed in at Agricultural Research Station, Mandor, Jodhpur, Rajasthan, from 2019. The related to infection first recorded after 70-75 days sowing month January years 2019 2022. major yellowing, phyllody, witches-broom, yellowing deformed elongated seeds. Disease incidence was as 0.25-1.0%, 0.5-1.5%, 0.5-2.5 % 0.5-10.6% during 2019, 2020, 2021 2022, respectively using quadrate method. In among different genotypes assessed GC 4, MCU 73, 105, 32 exhibited lower disease incidences ranging 0.5% 1.5%, while 78 highest 10.6%. To detect association symptomatic samples, genomic DNA extracted four representative (CuPP-MND-01 CuPP-MND-04) one asymptomatic plant Qiagen DNeasy mini kit (Germany). amplified nested PCR assays universal detection primers 16S rRNA gene (P1/P7 R16F2n/R16R2) (Schneider et al., 1995; Gundersen Lee, 1996) secA specific (SecAfor1/SecArev3 followed by SecAfor5/ SecArev2) (Hodgetts al. 2008; Bekele 2011). amplicons ∼1.25 kb ∼600 bp all samples positive control brinjal little leaf. products selected gene, sequenced both ends. 1,245 sequences deposited GenBank (OQ299007-10), which showed 100% sequence identity each other 99.4% 'Candidatus Phytoplasma citri' reference strain (GenBank accession: U15442) (Rodrigues Jardim 2023). phylogenetic analysis virtual RFLP 17 restriction enzymes through iPhyclassifier allowed affiliating strains 16SrII-C subgroup a similarity coefficient 1 pattern 16Sr group II, C AJ293216) (Zhao 2009). addition, gene-based (OQ305073-76) further confirmed close phytoplasmas phyllody witches' broom cumin. Earlier has been reported various crops weeds (Rao However, no earlier abroad. best our knowledge, this report on causing genotypes. This economic epidemiological implications needs immediate attention reduce export losses due prevent potential spread crops.

Язык: Английский

Процитировано

0