Longitudinal genomic surveillance of a UK intensive care unit shows a lack of patient colonisation by multi-drug-resistant Gram-negative bacterial pathogens DOI Creative Commons
Ann E. Snaith, Robert A. Moran, Rebecca J Hall

и другие.

Microbial Genomics, Год журнала: 2024, Номер 10(11)

Опубликована: Ноя. 4, 2024

Vulnerable patients in an intensive care unit (ICU) setting are at high risk of infection from bacteria including gut-colonising Escherichia coli and Klebsiella species. Complex ICU procedures often depend on successful antimicrobial treatment, underscoring the importance understanding extent patient colonisation by multi-drug-resistant organisms (MDROs) large UK ICUs. Previous work ICUs globally uncovered rates transmission MDROs, but situation is less understood. Here, we investigated diversity antibiotic resistance gene (ARG) carriage present one largest Queen Elizabeth Hospital Birmingham (QEHB), focusing primarily E. as both a widespread commensal disseminated pathogen. Samples were taken during highly restrictive coronavirus disease 2019 (COVID-19) control measures May to December 2021. Whole-genome metagenomic sequencing used detect report strain-level patients, sequence types (STs), their dynamics carriage. We found lack multi-drug (MDR) QEHB. Only carbapenemase-producing organism was isolated, Citrobacter carrying bla KPC-2 . There no evidence supporting spread this strain, there little overall nosocomial acquisition or circulation colonising Whilst 22 different STs identified, only 1 strain pandemic ST131 lineage isolated. This non-MDR be clade A associated with low levels resistance. Overall, QEHB had very MDR strains, result that may influenced part strict COVID-19 place time. Employing some these prevention where reasonable all might therefore assist maintaining MDR.

Язык: Английский

Chlorine in cleaning – are we concentrating enough? DOI
M.I. Garvey,

Faith Offorbuzor,

M. Wilkinson

и другие.

Journal of Hospital Infection, Год журнала: 2024, Номер unknown

Опубликована: Окт. 1, 2024

Язык: Английский

Процитировано

0
Distribution Patterns of tfdI and tfdII Gene Clusters and New Insights into the Formation of the Architecture of pJP4, a Canonical 2,4-dichlorophenoxyacetic Acid (2,4-D) Degradation Plasmid DOI Open Access
Т. Р. Ясаков

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(20), С. 10998 - 10998

Опубликована: Окт. 12, 2024

Currently, pJP4 is one of the best-known plasmids for biodegradation xenobiotics that mediate degradation 2,4-dichlorophenoxyacetic acid (2,4-D), which associated with serious health and environmental risks. Although sequencing proposed theory formation occurred almost 20 years ago (2004), still model object many studies focused on 2,4-D. The uniqueness this plasmid due to presence two evolutionarily distinct gene clusters, tfdI tfdII, controlling Recent advances in biology, especially those concerning characterization new IncP-1 systematization tfd cluster findings, serve as a basis proposing insights into clusters’ architecture canonical plasmid, pJP4, their distribution among other plasmids. In present work, comparative genomic phylogenetic silico study tfdII clusters was carried out. possible initial patterns different incompatibility groups (non-IncP-1) using IS1071-based composite transposon were revealed. A through sequential internal rearrangements, recombination, ISJP4 insertion, proposed. addition, small resulting from rearrangements (tfdIISA ORF31/32) served fingerprints exploring clusters. revealed formulated extend frontiers biology will be beneficial understanding role bacterial adaptation xenobiotic-contaminated environments.

Язык: Английский

Процитировано

0
Longitudinal genomic surveillance of a UK intensive care unit shows a lack of patient colonisation by multi-drug-resistant Gram-negative bacterial pathogens DOI Creative Commons
Ann E. Snaith, Robert A. Moran, Rebecca J Hall

и другие.

Microbial Genomics, Год журнала: 2024, Номер 10(11)

Опубликована: Ноя. 4, 2024

Vulnerable patients in an intensive care unit (ICU) setting are at high risk of infection from bacteria including gut-colonising Escherichia coli and Klebsiella species. Complex ICU procedures often depend on successful antimicrobial treatment, underscoring the importance understanding extent patient colonisation by multi-drug-resistant organisms (MDROs) large UK ICUs. Previous work ICUs globally uncovered rates transmission MDROs, but situation is less understood. Here, we investigated diversity antibiotic resistance gene (ARG) carriage present one largest Queen Elizabeth Hospital Birmingham (QEHB), focusing primarily E. as both a widespread commensal disseminated pathogen. Samples were taken during highly restrictive coronavirus disease 2019 (COVID-19) control measures May to December 2021. Whole-genome metagenomic sequencing used detect report strain-level patients, sequence types (STs), their dynamics carriage. We found lack multi-drug (MDR) QEHB. Only carbapenemase-producing organism was isolated, Citrobacter carrying bla KPC-2 . There no evidence supporting spread this strain, there little overall nosocomial acquisition or circulation colonising Whilst 22 different STs identified, only 1 strain pandemic ST131 lineage isolated. This non-MDR be clade A associated with low levels resistance. Overall, QEHB had very MDR strains, result that may influenced part strict COVID-19 place time. Employing some these prevention where reasonable all might therefore assist maintaining MDR.

Язык: Английский

Процитировано

0