bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2022, Номер unknown
Опубликована: Фев. 16, 2022
Transposons, which are DNA sequences that can move to new positions in the genome, make up a large fraction of eukaryotic genomes and occur clusters. The insertion transposons into genome is hindered by compact folding chromatin, supposedly preventing aberrant or even pathogenic insertion. Chromatin can, however, be decompacted as consequence transposon insertion, leading increased accessibility and, consequence, further insertions. While these observations suggest positive feedback between chromatin unfolding how such might contribute clustered remains poorly understood. In this study, we analyze polymer models self-interacting domain unfolds increasing numbers inserted block self-interaction. On one hand, find that, if additional adjacently already transposons, changes from sharp globule-coil transition more gradual extension loops core folded. other adjacent emerges either when transposases excluded densely packed proceeds very quickly relation thermal equilibration configurations. We thus derive our model physical conditions for resulting spatial compartmentalization chromatin. An according role was recently suggested LINE-1 Alu repeats, clusters drive mesoscopic mammalian genome. Significance Statement A part composed repetitive sequences, so-called transposons. Transposons involved important processes, early embryonic development control over genes used cell. frequently clusters, where many similar sequence motifs grouped together. Recent studies result local favoring yet Our work simulates simplified region transposases, molecules insert Surprisingly, fast-acting favor formation distinct contain most providing potential explanation
Язык: Английский
