mRNA capping enzyme exports to cytoplasm, localizes to stress granules and maintains cap homeostasis of the target mRNAs DOI Open Access
Anakshi Gayen, Avik Mukherjee, Shubhra Majumder

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Март 28, 2023

Abstract mRNA decapping is believed to trigger RNA degradation until the identification of cytoplasmic capping that has changed epitome stability. Unlike nuclear machinery includes polymerase II bound Capping Enzyme (CE), N-7 methyl transferase and RNMT activating protein RAM, complex consist pool CE (cCE) transferase-RAM along with a few proteins various functions. Cytoplasmic been shown recap selective uncapped mRNAs maintains cap homeostasis by cyclic process recapping. Thus, it acts as post-transcriptional nexus for target transcripts. Our data show export mammalian regulated Exportin1 (XPO1) pathway via conserved Nuclear Export Signal sequence. In order examine biological function cCE, we cCE forms granules during stress majority these co-localize SGs. identify how regulates recovery, measured status specific targeted transcripts non-targeted non-stress, recovery phase using Xrm1 susceptibility assay. lost their caps in condition when sequestered granules. After removal stress, released, restored pointing towards role altering thus promoting cellular from stress.

Язык: Английский

mRNA capping enzyme exports to cytoplasm, localizes to stress granules and maintains cap homeostasis of the target mRNAs DOI Open Access
Anakshi Gayen, Avik Mukherjee, Shubhra Majumder

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Март 28, 2023

Abstract mRNA decapping is believed to trigger RNA degradation until the identification of cytoplasmic capping that has changed epitome stability. Unlike nuclear machinery includes polymerase II bound Capping Enzyme (CE), N-7 methyl transferase and RNMT activating protein RAM, complex consist pool CE (cCE) transferase-RAM along with a few proteins various functions. Cytoplasmic been shown recap selective uncapped mRNAs maintains cap homeostasis by cyclic process recapping. Thus, it acts as post-transcriptional nexus for target transcripts. Our data show export mammalian regulated Exportin1 (XPO1) pathway via conserved Nuclear Export Signal sequence. In order examine biological function cCE, we cCE forms granules during stress majority these co-localize SGs. identify how regulates recovery, measured status specific targeted transcripts non-targeted non-stress, recovery phase using Xrm1 susceptibility assay. lost their caps in condition when sequestered granules. After removal stress, released, restored pointing towards role altering thus promoting cellular from stress.

Язык: Английский

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