Dynamic fibronectin assembly and remodeling by leader neural crest cells prevents jamming in collective cell migration
eLife,
Год журнала:
2023,
Номер
12
Опубликована: Апрель 19, 2023
Collective
cell
migration
plays
an
essential
role
in
vertebrate
development,
yet
the
extent
to
which
dynamically
changing
microenvironments
influence
this
phenomenon
remains
unclear.
Observations
of
distribution
extracellular
matrix
(ECM)
component
fibronectin
during
loosely
connected
neural
crest
cells
(NCCs)
lead
us
hypothesize
that
NCC
remodeling
initially
punctate
ECM
creates
a
scaffold
for
trailing
cells,
enabling
them
form
robust
and
coherent
stream
patterns.
We
evaluate
idea
theoretical
setting
by
developing
individual-based
computational
model
incorporates
reciprocal
interactions
between
NCCs
their
ECM.
remodeling,
haptotaxis,
contact
guidance,
cell-cell
repulsion
are
sufficient
establish
streams
silico,
however,
additional
mechanisms,
such
as
chemotaxis,
required
consistently
guide
along
correct
target
corridor.
Further
investigations
imply
guidance
differential
leader
follower
key
contributors
collective
preventing
breakage.
Global
sensitivity
analysis
simulated
gain-
loss-of-function
experiments
suggest
long-distance
without
jamming
is
most
likely
occur
when
leading
specialize
creating
fibers,
responding
environmental
cues
upregulating
mechanisms
guidance.
Язык: Английский
PhysiMeSS - a new physiCell addon for extracellular matrix modelling
Gigabyte,
Год журнала:
2024,
Номер
2024
Опубликована: Окт. 16, 2024
The
extracellular
matrix,
composed
of
macromolecules
like
collagen
fibres,
provides
structural
support
to
cells
and
acts
as
a
barrier
that
metastatic
degrade
spread
beyond
the
primary
tumour.
While
agent-based
frameworks,
such
PhysiCell,
can
simulate
spatial
dynamics
tumour
evolution,
they
only
implement
circles
(2D)
or
spheres
(3D).
To
model
matrix
network
we
require
new
type
agent
represented
by
line
segments
cylinders
Here,
present
PhysiMeSS,
an
addon
introducing
describe
fibres
their
physical
interactions
with
other
fibres.
PhysiMeSS
implementation
is
available
at
https://github.com/PhysiMeSS/PhysiMeSS
in
official
PhysiCell
repository.
We
provide
examples
describing
possibilities
this
framework.
This
tool
may
help
tackle
important
biological
questions,
diseases
linked
dysregulation
processes
leading
cancer
metastasis.
Язык: Английский
Drug-loaded nanoparticles for cancer therapy: a high-throughput multicellular agent-based modeling study
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Апрель 12, 2024
Interactions
between
biological
systems
and
engineered
nanomaterials
have
become
an
important
area
of
study
due
to
the
application
in
medicine.
In
particular,
for
cancer
diagnosis
or
treatment
presents
a
challenging
opportunity
complex
biology
this
disease
spanning
multiple
time
spatial
scales.
A
system-level
analysis
would
benefit
from
mathematical
modeling
computational
simulation
explore
interactions
anticancer
drug-loaded
nanoparticles
(NPs),
cells,
tissues,
associated
parameters
driving
system
patient's
overall
response.
Although
number
models
explored
these
past,
few
focused
on
simulating
individual
cell-NP
interactions.
This
develops
multicellular
agent-based
model
nanotherapy
that
simulates
NP
internalization,
drug
release
within
cell
cytoplasm,
"inheritance"
NPs
by
daughter
cells
at
division,
pharmacodynamic
response
intracellular
drug,
effect
tumor
dynamics.
large-scale
parallel
framework
is
used
investigate
impact
pharmacokinetic
design
(NP
internalization
rate,
decay
rate)
therapeutic
strategies
doses
injection
frequency)
through
exploration
results
indicate
may
be
improved
when
are
inherited
division
Язык: Английский
PhysiMeSS - A New PhysiCell Addon for Extracellular Matrix Modelling
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Окт. 27, 2023
Abstract
The
extracellular
matrix
is
a
complex
assembly
of
macro-molecules,
such
as
collagen
fibres,
which
provides
structural
support
for
surrounding
cells.
In
the
context
cancer
metastasis,
it
represents
barrier
cells,
that
migrating
cells
needs
to
degrade
in
order
leave
primary
tumor
and
invade
further
tissues.
Agent-based
frameworks,
PhysiCell,
are
often
use
represent
spatial
dynamics
evolution.
However,
typically
they
only
implement
agents,
represented
by
either
circle
(2D)
or
sphere
(3D).
accurately
network
we
require
new
type
agent
segment
cylinder
this
article,
present
PhysiMeSS,
an
addon
introduces
describe
their
physical
interactions
with
other
fibres.
PhysiMeSS
implementation
publicly
available
at
https://github.com/PhysiMeSS/PhysiMeSS
,
well
official
Physi-Cell
repository.
We
also
provide
simple
examples
extended
possibilities
framework.
hope
tool
will
serve
tackle
important
biological
questions
diseases
linked
dis-regulation
matrix,
processes
leading
metastasis.
Язык: Английский