Integrative Transcriptomic Profiling of NK Cells and Monocytes: Advancing Diagnostic and Therapeutic Strategies for COVID-19. DOI Creative Commons

Salma LOUKMAN,

Reda Ben Mrid,

Najat Bouchmaa

и другие.

Опубликована: Окт. 21, 2024

Abstract In this study, we use integrated transcriptomic datasets from the GEO repository with purpose of investigating immune dysregulation in COVID-19. Thus, context, decided to be focused on NK cells and CD14+ monocytes gene expression, considering GSE165461 GSE198256, respectively. Other PBMCs, lung, olfactory, sensory epithelium lymph were used provide robust validation for our results. This approach gave an view responses COVID-19, pointing out a set potential biomarkers therapeutic targets special regard standards physiological conditions. IFI27, MKI67, CENPF, MBP, HBA2, TMEM158, THBD, HBA1, LHFPL2, SLA, AC104564.3 identified as key genes analysis that have critical biological processes related inflammation, regulation, oxidative stress, metabolic processes. Consequently, such are important understanding heterogeneous clinical manifestations COVID-19—from acute long-term effects now known ‘long COVID’. Subsequent additional consolidated these role diagnosis COVID-19 prediction its severity. Moreover, their enrichment pathophysiological pathways presented them intervention.The results insight into molecular dynamics caused by other monocytes. study constitutes solid basis targeted diagnostic development makes relevant contributions ongoing research efforts toward better management mitigation pandemic.

Язык: Английский

Traumatic Brain Injury in the Long-COVID Era DOI Creative Commons
Denes V. Agoston

Neurotrauma Reports, Год журнала: 2024, Номер 5(1), С. 81 - 94

Опубликована: Янв. 1, 2024

Major determinants of the biological background or reserve, such as age, sex, comorbidities (diabetes, hypertension, obesity, etc.), and medications (e.g., anticoagulants), are known to affect outcome after traumatic brain injury (TBI). With unparalleled data richness coronavirus disease 2019 (COVID-19; ∼375,000 counting!) well chronic form, long-COVID, also called post-acute sequelae SARS-CoV-2 infection (PASC), publications (∼30,000 counting) covering virtually every aspect diseases, pathomechanisms, biomarkers, phases, symptomatology, etc., have provided a unique opportunity better understand appreciate holistic nature interconnectivity between organ systems, importance in modifying trajectories affecting outcomes. Such approach is badly needed TBI-induced conditions their totality. Here, I briefly review what about long-COVID/PASC, its underlying—suspected—pathologies, pathobiological changes induced by TBI, other words, TBI endophenotypes, discuss intersection long-COVID/PASC pathobiologies, how considering some factors person's inclusion mechanistic molecular biomarkers can help improve clinical management patients.

Язык: Английский

Процитировано

2

Cardiovascular symptoms of PASC are associated with trace-level cytokines that affect the function of human pluripotent stem cell derived cardiomyocytes DOI Creative Commons
Jane E. Sinclair,

Courtney Vedelago,

Feargal J. Ryan

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Апрель 12, 2024

ABSTRACT Globally, over 65 million individuals are estimated to suffer from post-acute sequelae of COVID-19 (PASC). A large number living with PASC experience cardiovascular symptoms (i.e. chest pain and heart palpitations) (PASC-CVS). The role chronic inflammation in these symptoms, particular persisting for >1 year after SARS-CoV-2 infection, remains be clearly defined. In this cross-sectional study, blood samples were obtained three different sites Australia i) a resolved infection (and no persistent i.e. ‘Recovered’), ii) prolonged PASC-CVS iii) negative individuals. Individuals PASC-CVS, relative Recovered individuals, had transcriptomic signature associated inflammation. This was accompanied by elevated levels pro-inflammatory cytokines (IL-12, IL-1β, MCP-1 IL-6) at approximately 18 months post-infection. These present trace amounts, such that they could only detected the use novel nanotechnology. Importantly, trace-level direct effect on functionality pluripotent stem cell derived cardiomyocytes vitro . not observed presence dexamethasone. Plasma proteomics demonstrated further differences between patients post-infection including enrichment complement coagulation proteins those symptoms. Together, data provide new insight into nanotechnology as possible diagnostic approach condition.

Язык: Английский

Процитировано

2

Long COVID is associated with lower percentages of mature, cytotoxic NK cell phenotypes DOI Creative Commons
Tasha Tsao, Amanda M. Buck,

Lucile Grimbert

и другие.

Journal of Clinical Investigation, Год журнала: 2024, Номер unknown

Опубликована: Дек. 17, 2024

Язык: Английский

Процитировано

2

Long COVID: G Protein-Coupled Receptors (GPCRs) Associated Genes and Pathways as a Promising Therapeutic Potential Through Computational Analysis DOI Open Access

Dhanyashri Guruparan,

Suresh Kumar

Опубликована: Авг. 8, 2024

Long COVID, also referred to as post-COVID-19 condition, describes the lingering after-effects of COVID-19 infection. This complex syndrome encompasses a range symptoms affecting physical well-being, cognitive function, and mental health. The widespread impact long COVID extends beyond individual sufferers, placing significant strain on healthcare systems causing far-reaching socioeconomic consequences. scale this multifaceted health crisis is unparalleled in recent history, millions globally challenging our understanding post-viral conditions. study aimed explore possibility targeting G protein-coupled receptors (GPCRs) signaling pathway-related genes therapeutics for using computational analysis. Targeting GPCRs their pathways holds promise therapeutics, these are involved crucial physiological processes including immune regulation, inflammation control, neurotransmission. All implicated diverse persistent potentially offering versatile approach addressing multiple aspects condition simultaneously. identified 21 associated with further exploration potential targets. Notably, GPCR such HRAS, KRAS, GNAQ, GNA11 were determined be possible therapeutic targets pathophysiological mechanisms COVID. FDA-approved drugs identified, Binimetinib, Cabozantinib, Selumetinib, Panitumumab, Cetuximab, Adagrasib, Tipifarnib, Sotorasib. Additionally, Naltrexone Tipifarnib have emerged new treatment options SARS-CoV-2 may hijack dysregulate lung ion fluid transport, contributing pathophysiology pulmonary edema patients COVID-19. Further research required translate findings into development investigate relationship between viral infection signalling.

Язык: Английский

Процитировано

1

Integrative Transcriptomic Profiling of NK Cells and Monocytes: Advancing Diagnostic and Therapeutic Strategies for COVID-19. DOI Creative Commons

Salma LOUKMAN,

Reda Ben Mrid,

Najat Bouchmaa

и другие.

Опубликована: Окт. 21, 2024

Abstract In this study, we use integrated transcriptomic datasets from the GEO repository with purpose of investigating immune dysregulation in COVID-19. Thus, context, decided to be focused on NK cells and CD14+ monocytes gene expression, considering GSE165461 GSE198256, respectively. Other PBMCs, lung, olfactory, sensory epithelium lymph were used provide robust validation for our results. This approach gave an view responses COVID-19, pointing out a set potential biomarkers therapeutic targets special regard standards physiological conditions. IFI27, MKI67, CENPF, MBP, HBA2, TMEM158, THBD, HBA1, LHFPL2, SLA, AC104564.3 identified as key genes analysis that have critical biological processes related inflammation, regulation, oxidative stress, metabolic processes. Consequently, such are important understanding heterogeneous clinical manifestations COVID-19—from acute long-term effects now known ‘long COVID’. Subsequent additional consolidated these role diagnosis COVID-19 prediction its severity. Moreover, their enrichment pathophysiological pathways presented them intervention.The results insight into molecular dynamics caused by other monocytes. study constitutes solid basis targeted diagnostic development makes relevant contributions ongoing research efforts toward better management mitigation pandemic.

Язык: Английский

Процитировано

1