Current Status and Future Strategies for Advancing Functional Circuit MappingIn Vivo DOI
A. Berndt, Denise J. Cai, Adam E. Cohen

и другие.

Journal of Neuroscience, Год журнала: 2023, Номер 43(45), С. 7587 - 7598

Опубликована: Ноя. 8, 2023

The human brain represents one of the most complex biological systems, containing billions neurons interconnected through trillions synapses. Inherent to is a biochemical complexity involving ions, signaling molecules, and peptides that regulate neuronal activity allow for short- long-term adaptations. Large-scale noninvasive imaging techniques, such as fMRI EEG, have highlighted regions involved in specific functions visualized connections between different areas. A major shortcoming, however, need more information on cell types neurotransmitters involved, well poor spatial temporal resolution. Recent technologies been advanced circuit mapping implemented behaving model organisms address this. Here, we highlight strategies targeting subtypes, identifying, releasing controlling gene expression, monitoring circuits real-time vivo . Combined, these approaches us establish direct causal links from genes molecules systems level ultimately cognitive processes.

Язык: Английский

CA1 Engram Cell Dynamics Before and After Learning DOI Creative Commons
Amy Monasterio, Caitlin Lienkaemper,

Siria Coello

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Апрель 20, 2024

Summary A fundamental question in neuroscience is how memory formation shapes brain activity at the level of populations neurons. Recent studies hippocampal ‘engram’ cells, identified by immediate-early genes (IEGs) induced learning, propose that these act as a neuronal substrate for storage. The current framework engram proposes cells join ensembles based on increased intrinsic excitability, and after initial they co-activate to support retrieval. However, direct evidence population dynamics evolve across learning limited. Here we combined activity-dependent genetic tagging two-photon calcium imaging characterize CA1 before learning. We observed spontaneous two days predicted tagging, consistent with model which fluctuations bias into forming assemblies. Surprisingly, were unable detect rates or pairwise correlations amongst tagged neurons These results computational network models incorporate strong specific inhibitory connections, supporting idea excitatory/inhibitory balance may play key role dynamics. Together highlight potential slow time scale excitability driving suggest excitatory-inhibitory regulate cell co-activation. Highlights Tracked Fos Spontaneous predicts TetTagging. No changes TetTagged Modeling suggests E/I regulates

Язык: Английский

Процитировано

14
Integrating and fragmenting memories under stress and alcohol DOI Creative Commons

Krystian Loetscher,

Elizabeth V. Goldfarb

Neurobiology of Stress, Год журнала: 2024, Номер 30, С. 100615 - 100615

Опубликована: Фев. 8, 2024

Stress can powerfully influence the way we form memories, particularly extent to which they are integrated or situated within an underlying spatiotemporal and broader knowledge architecture. These different representations in turn have significant consequences for use these memories guide later behavior. Puzzlingly, although stress has historically been argued promote fragmentation, leading disjoint memory representations, more recent work suggests that also facilitate binding integration. Understanding circumstances under fosters integration will be key resolving this discrepancy unpacking mechanisms by shape Here, examine at multiple levels: linking together content of individual experience, threading associations between related but distinct events, experience into a pre-existing schema sense causal structure. We discuss neural cognitive each as well findings regarding how stress, aversive learning, negative affect modulate each. In analysis, uncover indeed level show may apply understanding effects alcohol, highlighting extant clinical preclinical opportunities further investigation. Finally, consider implications fragmentation memory-guided behavior, importance type representation is potentiated order design appropriate interventions.

Язык: Английский

Процитировано

6
Engram mechanisms of memory linking and identity DOI
Ali Choucry, Masanori Nomoto, Kaoru Inokuchi

и другие.

Nature reviews. Neuroscience, Год журнала: 2024, Номер 25(6), С. 375 - 392

Опубликована: Апрель 25, 2024

Язык: Английский

Процитировано

5
Causal Spike Timing Dependent Plasticity Prevents Assembly Fusion in Recurrent Networks DOI Creative Commons
Xin‐She Yang, Brent Doiron

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 15, 2025

The organization of neurons into functionally related assemblies is a fundamental feature cortical networks, yet our understanding how these maintain distinct identities while sharing members remains limited. Here we analyze spike-timing-dependent plasticity (STDP) shapes the formation and stability overlapping neuronal in recurrently coupled networks spiking neuron models. Using numerical simulations an associated mean-field theory, demonstrate that temporal structure STDP rule, specifically its degree causality, critically determines whether share segregation or merge together after training completed. We find causal rules, where potentiation/depression occurs strictly when presynaptic spikes precede/proceed postsynaptic spikes, allow to remain even with substantial overlap membership. This arises because effectively cancels symmetric correlations introduced by common inputs from shared neurons. In contrast, acausal rules lead assembly fusion exceeds critical threshold, due unchecked growth input correlations. Our results provide theoretical insight learning can support distributed representation individual participate multiple maintaining functional specificity.

Язык: Английский

Процитировано

0
Prefrontal encoding of an internal model for emotional inference DOI Open Access

Xiao-Wei Gu,

Joshua P. Johansen

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Апрель 25, 2024

A key function of brain systems mediating emotion is to learn anticipate unpleasant experiences based on predictive sensory cues in the environment. While organisms readily associate stimuli with aversive outcomes, higher-order forms emotional learning and memory require inference extrapolate circumstances surrounding directly experienced events other indirectly related contexts patterns which weren’t a part original experience. To achieve this type requires internal models flexibly track inferred associations. mechanisms simple have been well studied areas such as amygdala, whether how represents emotionally relevant associations not known. Here we report that neurons rodent dorsomedial prefrontal cortex (dmPFC) encode an model by linking environment events, they were or associated These representations are flexible, updating behavioral significance individual features association selectively modifies corresponding dmPFC representations. population activity encodes all salient associations, projecting amygdala specifically represent required express Together, these findings reveal encoded regulate subcortical for recall memories.

Язык: Английский

Процитировано

1
Neurotransmitter switch-up helps fan extreme stress into full-blown fear DOI
Claudia López Lloreda

The Transmitter, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

Язык: Английский

Процитировано

0
Reactivation-dependent transfer of fear memory between contexts requires M1 muscarinic receptor stimulation in dorsal hippocampus DOI Creative Commons
Karim H. Abouelnaga, Andrew E. Huff,

Kristen H. Jardine

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 3, 2024

Abstract Background Memory updating is essential for integrating new information into existing representations. However, this process could become maladaptive in conditions like post-traumatic stress disorder (PTSD), when fear memories generalize to neutral contexts. Previously, we have shown that contextual memory malleability rats requires activation of M1 muscarinic acetylcholine receptors the dorsal hippocampus. Here, investigated involvement mechanism transfer other contexts using a novel paradigm. Methods Following brief re-exposure previously conditioned context, male (n=8-10/group) were placed context evaluate memory. We also infused selective receptor antagonist pirenzepine hippocampus prior reactivation try block effect. Results support hypothesis can be updated with information, but only if are re-exposed originally trained relatively recently context; evidence was not seen omitted or it occurred 6h exposure. The transferred persisted four weeks, and effect blocked by antagonism. Conclusions These findings strongly suggest destabilization original Specific parameters likely dictate similar generalization disorders PTSD. preclinical model introduced here, its implication process, should inform therapeutic strategies area.

Язык: Английский

Процитировано

0
CA1 Engram Cell Dynamics Before and After Learning DOI Creative Commons
Steve Ramirez, Amy Monasterio, Caitlin Lienkaemper

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Июнь 24, 2024

Abstract A fundamental question in neuroscience is how memory formation shapes brain activity at the level of populations neurons. Recent studies hippocampal ‘engram’ cells, identified by immediate-early genes (IEGs) induced learning, propose that these act as a neuronal substrate for storage. The current framework engram proposes cells join ensembles based on increased intrinsic excitability, and after initial they co-activate to support retrieval. However, direct evidence population dynamics evolve across learning limited. Here we combined activity-dependent genetic tagging two-photon calcium imaging characterize CA1 before learning. We observed spontaneous two days predicted tagging, consistent with model which fluctuations bias into forming assemblies. Surprisingly, were unable detect rates or pairwise correlations amongst tagged neurons These results computational network models incorporate strong specific inhibitory connections, supporting idea excitatory/inhibitory balance may play key role dynamics. Together highlight potential slow time scale excitability driving suggest excitatory-inhibitory regulate cell co-activation.

Язык: Английский

Процитировано

0
Reactivation-dependent transfer of fear memory between contexts requires M1 muscarinic receptor stimulation in dorsal hippocampus of male rats DOI Open Access
Karim H. Abouelnaga, Andrew E. Huff,

Kristen H. Jardine

и другие.

Learning & Memory, Год журнала: 2024, Номер 31(9), С. a054039 - a054039

Опубликована: Сен. 1, 2024

Memory updating is essential for integrating new information into existing representations. However, this process could become maladaptive in conditions like post-traumatic stress disorder (PTSD), when fear memories generalize to neutral contexts. Previously, we have shown that contextual memory malleability rats requires activation of M1 muscarinic acetylcholine receptors the dorsal hippocampus. Here, investigated involvement mechanism transfer other contexts using a novel paradigm. Following brief reexposure previously conditioned context, male (

Язык: Английский

Процитировано

0
Current Status and Future Strategies for Advancing Functional Circuit MappingIn Vivo DOI
A. Berndt, Denise J. Cai, Adam E. Cohen

и другие.

Journal of Neuroscience, Год журнала: 2023, Номер 43(45), С. 7587 - 7598

Опубликована: Ноя. 8, 2023

The human brain represents one of the most complex biological systems, containing billions neurons interconnected through trillions synapses. Inherent to is a biochemical complexity involving ions, signaling molecules, and peptides that regulate neuronal activity allow for short- long-term adaptations. Large-scale noninvasive imaging techniques, such as fMRI EEG, have highlighted regions involved in specific functions visualized connections between different areas. A major shortcoming, however, need more information on cell types neurotransmitters involved, well poor spatial temporal resolution. Recent technologies been advanced circuit mapping implemented behaving model organisms address this. Here, we highlight strategies targeting subtypes, identifying, releasing controlling gene expression, monitoring circuits real-time vivo . Combined, these approaches us establish direct causal links from genes molecules systems level ultimately cognitive processes.

Язык: Английский

Процитировано

1