Networks of descending neurons transform command-like signals into population-based behavioral control

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Сен. 12, 2023

Abstract To transform intentions into actions, movement instructions must pass from the brain to downstream motor circuits through descending neurons (DNs). These include small sets of command-like that are sufficient drive behaviors—the circuit mechanisms for which remain unclear. Here, we show DNs in Drosophila directly recruit networks additional orchestrate flexible behaviors. Specifically, found optogenetic activation previously thought behaviors alone fact co-activate larger populations DNs. Connectome analysis revealed this functional recruitment can be explained by direct excitatory connections between and interconnected brain. The size DN is predictive whether population necessary generate a complete behavior: with many partners require network behaviors, while fewer stereotyped simple movements. Finally, reside within behavior-specific clusters inhibit one another. results support mechanism control whereby continuum generated increasingly large likely construct behavior combining multiple subroutines.

Язык: Английский

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Chemical cognition: chemoconnectomics and convergent evolution of integrative systems in animals

Animal Cognition, Год журнала: 2023, Номер 26(6), С. 1851 - 1864

Опубликована: Ноя. 1, 2023

Язык: Английский

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Molecular control of temporal integration matches decision-making to motivational state

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Март 5, 2024

Motivations bias our responses to stimuli, producing behavioral outcomes that match needs and goals. We describe a mechanism behind this phenomenon: adjusting the time over which stimulus-derived information is permitted accumulate toward decision. As Drosophila copulation progresses, male becomes less likely continue mating through challenges. show set of Copulation Decision Neurons (CDNs) flexibly integrates about competing drives mediate Early in mating, dopamine signaling restricts CDN integration by potentiating CaMKII activation response stimulatory inputs, imposing high threshold for changing behaviors. Later into timescale CDNs integrate termination-promoting expands, increasing likelihood switching suggest scalable windows temporal at dedicated circuit nodes as key but underappreciated variable state-based decision-making.

Язык: Английский

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Systematic annotation of a complete adult male Drosophila nerve cord connectome reveals principles of functional organisation

Опубликована: Июль 22, 2024

Nervous systems function as ensembles of neurons communicating via synaptic connections, and a functional understanding nervous requires extensive knowledge their connectomes. In companion paper (Takemura et al., 2023), we describe the acquisition complete fruit fly nerve cord connectome, first for an animal that can walk or fly. Here, to efficiently navigate appreciate biological significance this categorise name nearly all systematically link them experimental literature. We employ system hierarchical coarse annotations group similar across midline segments, then define systematic cell types sensory neurons, intrinsic ascending non-motor efferent neurons. Stereotyped arrays neuroblasts generate related neuron populations called hemilineages repeat segments cord. confirm larval-born from given hemilineage generally express same neurotransmitter but find earlier born often different one. match over 35% intrinsic, ascending, defining serial sets which were crucial typing motor assign modality 5000 cluster by connectivity, identify serially homologous layered organisation likely corresponding peripheral topography. Finally, present selected examples circuits predicated on programmatic analysis VNC connectome. Our are critical analysing structure descending input output, both described in third (Cheong 2023). These being released part neuprint.janelia.org clio.janelia.org web applications also serve basis connectome through dedicated tools paper.

Язык: Английский

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Author response: A split-GAL4 driver line resource for Drosophila CNS cell types

Опубликована: Июль 30, 2024

Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. In addition stocks for well-characterized lines, we make available 300,000 new 3D lines.

Язык: Английский

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