Single-cell morphometrics reveals T-box gene-dependent patterns of epithelial tension in the Second Heart field
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Ноя. 4, 2024
Abstract
The
vertebrate
heart
tube
extends
by
progressive
addition
of
epithelial
second
field
(SHF)
progenitor
cells
from
the
dorsal
pericardial
wall.
interplay
between
mechanics
and
genetic
mechanisms
during
SHF
deployment
is
unknown.
Here,
we
present
a
quantitative
single-cell
morphometric
analysis
extension,
including
force
inference
stress.
Joint
spatial
Principal
Component
Analysis
reveals
that
cell
orientation
stress
direction
are
main
parameters
defining
apical
morphology
distinguishes
adjacent
to
arterial
venous
poles.
Cell
shape
mechanical
forces
display
dynamic
relationship
formation.
Moreover,
while
T-box
transcription
factor
Tbx1
necessary
for
towards
pole,
activation
Tbx5
in
posterior
correlates
with
establishment
deletion
relaxes
epithelium.
Integrating
findings
cell-scale
feature
patterning
provides
new
insights
into
cardiac
morphogenesis.
Язык: Английский
An expanded view of cell competition
Development,
Год журнала:
2024,
Номер
151(22)
Опубликована: Ноя. 15, 2024
ABSTRACT
Cell
competition
arises
in
heterogeneous
tissues
when
neighbouring
cells
sense
their
relative
fitness
and
undergo
selection.
It
has
been
a
challenge
to
define
contexts
which
cell
is
physiologically
relevant
phenomenon
understand
the
cellular
features
that
underlie
sensing.
Drawing
on
examples
across
range
of
length
scales,
we
illuminate
molecular
could
diverse
tissue
types
processes
promote
reinforce
long-term
maintenance
function.
We
propose
by
broadening
scope
how
defined
circumstances
can
occur,
field
unlock
potential
as
lens
through
heterogeneity
its
role
fundamental
principles
complex
organisation
be
understood.
Язык: Английский
Peeking into the future: inferring mechanics in dynamical tissues
Biochemical Society Transactions,
Год журнала:
2024,
Номер
52(6), С. 2579 - 2592
Опубликована: Дек. 10, 2024
Cells
exert
forces
on
each
other
and
their
environment,
shaping
the
tissue.
The
resulting
mechanical
stresses
can
be
determined
experimentally
or
estimated
computationally
using
stress
inference
methods.
Over
years,
has
become
a
non-invasive,
low-cost
computational
method
for
estimating
relative
intercellular
intracellular
pressures
of
tissues.
This
mini-review
introduces
compares
static
dynamic
modalities
inference,
considering
advantages
limitations.
To
date,
most
software
focused
which
requires
only
single
microscopy
image
as
input.
Although
applicable
in
quasi-equilibrium
states,
this
approach
neglects
influence
that
cell
rearrangements
might
have
inference.
In
contrast,
relies
time
series
images
to
estimate
pressures.
Here,
we
discuss
both
terms
physical,
mathematical,
foundations
then
outline
what
believe
are
promising
avenues
silico
states
Язык: Английский