
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Июль 25, 2024
Abstract Development is a highly complex process consisting of coordinated cell proliferation, differentiation and spatial organization. Classically, two ways to specify types during development are hypothesized : mosaic regulative modes. In the mode, particular isolated from rest embryo will nevertheless give rise cells with fate identical ones expected in normal development, thus relying on lineage-inherited factors. depends its interactions environment, relies space-dependant Both modes often coexist given animal. We propose quantify their respective contributions single-cell trajectories. C. elegans provides unique opportunity elaborate such an approach. Indeed, invariant lineage enables integration positions, relationships protein expression data. Using single profile as readout state, we relate following measurable quantities. The contribution or quantified by strength relationship between cell-cell distance . Similarly, context-dependent context measures similarity neighborhoods based gene profiles neighbours. assess significance these comparing empirical results obtained data artificial models generated using simple rules. With measures, show co-existence relative varies across different tissues time. particular, see skin tissue that early mode dominates while at later stages, dominates, suggesting convergence These general can be applied other datasets made available progress transcriptomics lineage-tracing, paving way for quantitative, unbiased perturbation-free study fundamental concepts developmental biology.
Язык: Английский