A feedback-driven IoT microfluidic, electrophysiology, and imaging platform for brain organoid studies DOI Creative Commons
Kateryna Voitiuk, Spencer T. Seiler, Mirella Pessoa de Melo

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Март 17, 2024

Abstract The analysis of tissue cultures, particularly brain organoids, requires a sophisticated integration and coordination multiple technologies for monitoring measuring. We have developed an automated research platform enabling independent devices to achieve collaborative objectives feedback-driven cell culture studies. Our approach enables continuous, communicative, non-invasive interactions within Internet Things (IoT) architecture among various sensing actuation devices, achieving precisely timed control in vitro biological experiments. framework integrates microfluidics, electrophysiology, imaging maintain cerebral cortex organoids while measuring their neuronal activity. are cultured custom, 3D-printed chambers affixed commercial microelectrode arrays. Periodic feeding is achieved using programmable microfluidic pumps. computer vision fluid volume estimator used as feedback rectify deviations perfusion during media feeding/aspiration cycles. validated the system with set 7-day studies mouse comparing manual protocols. protocols were maintaining robust neural activity throughout experiment. enabled hourly electrophysiology recordings Median unit firing rates increased every sample dynamic patterns organoid revealed by high-frequency recordings. Surprisingly, did not affect rate. Furthermore, performing exchange recording showed no acute effects on rate, use this reagent screening

Язык: Английский

A feedback-driven IoT microfluidic, electrophysiology, and imaging platform for brain organoid studies DOI Creative Commons
Kateryna Voitiuk, Spencer T. Seiler, Mirella Pessoa de Melo

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Март 17, 2024

Abstract The analysis of tissue cultures, particularly brain organoids, requires a sophisticated integration and coordination multiple technologies for monitoring measuring. We have developed an automated research platform enabling independent devices to achieve collaborative objectives feedback-driven cell culture studies. Our approach enables continuous, communicative, non-invasive interactions within Internet Things (IoT) architecture among various sensing actuation devices, achieving precisely timed control in vitro biological experiments. framework integrates microfluidics, electrophysiology, imaging maintain cerebral cortex organoids while measuring their neuronal activity. are cultured custom, 3D-printed chambers affixed commercial microelectrode arrays. Periodic feeding is achieved using programmable microfluidic pumps. computer vision fluid volume estimator used as feedback rectify deviations perfusion during media feeding/aspiration cycles. validated the system with set 7-day studies mouse comparing manual protocols. protocols were maintaining robust neural activity throughout experiment. enabled hourly electrophysiology recordings Median unit firing rates increased every sample dynamic patterns organoid revealed by high-frequency recordings. Surprisingly, did not affect rate. Furthermore, performing exchange recording showed no acute effects on rate, use this reagent screening

Язык: Английский

Процитировано

3