bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Март 9, 2024

Purpose Cervical and endometrial cancers pose significant challenges in women’s healthcare due to their high mortality rates limited treatment options. High throughput screening (HTS) of cervical cancer vitro models offer a promising avenue for drug repurposing broadening patient Traditional two-dimensional (2D) cell-based screenings have capabilities capture crucial multicellular interactions, that are improved upon three dimensional (3D) tissue engineered models. However, manual fabrication the 3D platforms is both time consuming subject variability. Thus, goal this study was utilize automated cell dispensing fabricate HTS using HP D100 Single Cell Dispenser dispense cells. Methods We evaluated effects lines by tuning protocol align with size measured solution minimum number acceptable viability proliferation. modified our previously reported coculture be 384 well plate format microvessel length invasion. Results Automatically manually dispensed cells were directly compared revealing minimal differences between methods. These findings suggest minimally affects behavior can deployed decrease model time. Conclusions By streamlining manufacturing process, holds promise enhancing efficiency scalability platforms, ultimately contributing advancement research treatment.

Язык: Английский