Instrumentation at the Leading Edge of Proteomics
Analytical Chemistry,
Год журнала:
2024,
Номер
96(20), С. 7976 - 8010
Опубликована: Май 13, 2024
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ISSUEPREVReviewNEXTInstrumentation
at
the
Leading
Edge
of
ProteomicsTrenton
M.
Peters-ClarkeTrenton
Peters-ClarkeDepartment
Chemistry,
University
Wisconsin─Madison,
Madison,
Wisconsin
53706,
United
StatesDepartment
Biomolecular
StatesMore
by
Trenton
Peters-ClarkeView
Biographyhttps://orcid.org/0000-0002-9153-2525,
Joshua
J.
CoonJoshua
CoonDepartment
StatesMorgridge
Institute
for
Research,
53715,
CoonView
Biographyhttps://orcid.org/0000-0002-0004-8253,
and
Nicholas
Riley*Nicholas
RileyDepartment
Washington,
Seattle,
Washington
98195,
States*Email:
[email
protected]More
RileyView
Biographyhttps://orcid.org/0000-0002-1536-2966Cite
this:
Anal.
Chem.
2024,
96,
20,
7976–8010Publication
Date
(Web):May
13,
2024Publication
History
Received6
October
2023Accepted19
April
2024Revised17
2024Published
online13
May
inissue
21
2024https://pubs.acs.org/doi/10.1021/acs.analchem.3c04497https://doi.org/10.1021/acs.analchem.3c04497review-articleACS
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©
2024
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SUBJECTS:Dissociation,Ions,Mass
spectrometry,Peptides
proteins,Proteomics
Get
e-Alerts
Язык: Английский
Development of an efficient, effective, and economical technology for proteome analysis
Cell Reports Methods,
Год журнала:
2024,
Номер
4(6), С. 100796 - 100796
Опубликована: Июнь 1, 2024
We
present
an
efficient,
effective,
and
economical
approach,
named
E3technology,
for
proteomics
sample
preparation.
By
immobilizing
silica
microparticles
into
the
polytetrafluoroethylene
matrix,
we
develop
a
robust
membrane
medium,
which
could
serve
as
reliable
platform
to
generate
proteomics-friendly
samples
in
rapid
low-cost
fashion.
benchmark
its
performance
using
different
formats
demonstrate
them
with
variety
of
types
varied
complexity,
quantity,
volume.
Our
data
suggest
that
E3technology
provides
proteome-wide
identification
quantitation
equivalent
or
superior
many
existing
methods.
further
propose
enhanced
single-vessel
E4technology,
performs
on-filter
in-cell
digestion
minimal
loss
high
sensitivity,
enabling
low-input
low-cell
proteomics.
Lastly,
utilized
above
technologies
investigate
RNA-binding
proteins
profile
intact
bacterial
cell
proteome.
Язык: Английский