Hippocampal mGluR5 levels are comparable in Alzheimer′s and control brains, and divergently influenced by amyloid and tau in control brain DOI Creative Commons
Junlong Wang,

Serena Savodalli,

Yanyan Kong

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 26, 2024

Abstract Background Metabotropic glutamate receptor 5 (mGluR5) modulates excitatory glutamatergic synaptic transmission and plays an important role in learning memory formation neurodegeneration amyloid deposition Alzheimer’s disease (AD). Conflicting results on the cerebral mGluR5 levels AD have been reported based vivo postmortem studies. Here, we aimed to assess alterations hippocampal expression AD, associations between pathologies. Methods Immunofluorescence staining for was performed brain tissue from 34 patients 31 nondemented controls (NCs) aged 3×Tg arcAβ model mice of AD. Autoradiography slices using tracer [ 18 F]PSS232. Analysis different cellular source GRM5 RNA human mouse brains performed. Proteomic profiling pathway analysis were wild-type mice. Results No differences or entorhinal cortical detected NC groups. Hippocampal increased with Braak stage decreased level group. correlations amyloid, tau, Iba1/P2X7R hippocampus cases. Ex autoradiography revealed comparable F]PSS232 compared nontransgenic GO KEGG enrichment analyses that Shank3, Grm5 pathways upregulated Conclusion This study no difference NCs divergent influence tau pathologies NCs. Species observed as well at location. Graphical abstract

Язык: Английский

Hippocampal mGluR5 levels are comparable in Alzheimer′s and control brains, and divergently influenced by amyloid and tau in control brain DOI Creative Commons
Junlong Wang,

Serena Savodalli,

Yanyan Kong

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 26, 2024

Abstract Background Metabotropic glutamate receptor 5 (mGluR5) modulates excitatory glutamatergic synaptic transmission and plays an important role in learning memory formation neurodegeneration amyloid deposition Alzheimer’s disease (AD). Conflicting results on the cerebral mGluR5 levels AD have been reported based vivo postmortem studies. Here, we aimed to assess alterations hippocampal expression AD, associations between pathologies. Methods Immunofluorescence staining for was performed brain tissue from 34 patients 31 nondemented controls (NCs) aged 3×Tg arcAβ model mice of AD. Autoradiography slices using tracer [ 18 F]PSS232. Analysis different cellular source GRM5 RNA human mouse brains performed. Proteomic profiling pathway analysis were wild-type mice. Results No differences or entorhinal cortical detected NC groups. Hippocampal increased with Braak stage decreased level group. correlations amyloid, tau, Iba1/P2X7R hippocampus cases. Ex autoradiography revealed comparable F]PSS232 compared nontransgenic GO KEGG enrichment analyses that Shank3, Grm5 pathways upregulated Conclusion This study no difference NCs divergent influence tau pathologies NCs. Species observed as well at location. Graphical abstract

Язык: Английский

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