A new flavor of synthetic yeast communities sees the light

mBio, Год журнала: 2025, Номер unknown

Опубликована: Фев. 6, 2025

ABSTRACT No organism is an island: organisms of varying taxonomic complexity, including genetic variants a single species, can coexist in particular niches, cooperating for survival while simultaneously competing environmental resources. In recent years, synthetic biology strategies have witnessed surge efforts focused on creating artificial microbial communities to tackle pressing questions about the complexity natural systems and interactions that underpin them. These engineered ecosystems depend number nature their members, allowing complex cell communication designs recreate create diverse interest. Due its experimental simplicity, budding yeast Saccharomyces cerevisiae has been harnessed establish mixture varied populations with potential explore ecology, metabolic bioprocessing, biosensing, pattern formation. Indeed, enable advanced molecule detection dynamics logic operations. Here, we present concise overview state-of-the-art, highlighting examples exploit optogenetics manipulate, through light stimulation, key phenotypes at community level, unprecedented spatial temporal regulation. Hence, envision bright future where application optogenetic approaches (optoecology) illuminates intricate drives innovations engineering strategies.

Язык: Английский

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Minimal integrating shuttle vectors for Saccharomyces cerevisiae depleted of restriction sites outside the polylinker region

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 5, 2024

ABSTRACT Many plasmids harbor unnecessary elements that complicate or hinder cloning tasks such as inserting one gene into another for protein domain grafting. In particular, restriction sites may be present in the backbone outside polylinker region (multiple site; MCS) and thus unavailable use, overall length of a plasmid correlates with poorer ligation efficiency. To address these concerns, there has been growing interest minimal plasmids. Here, we describe design validation collection six integrating shuttle vectors genetic manipulation Saccharomyces cerevisiae . We constructed using de novo synthesis consisting only yeast selection marker ( HIS3, TRP1, LEU2, URA3, natMX6 , KanMX ), bacterial (Ampicillin resistance), an origin replication (ORI), MCS flanked by M13 forward reverse sequences. use truncated variants where available eliminated all other sequences typically found The consists ten unique sites. our knowledge, at sizes ranging from approximately 2.6 kb to 3.5 kb, are smallest described yeast. Further, removed common open reading frames (ORFs) terminators, freeing up 30 cut each plasmid. named pLS series accordance well-known pRS vectors, which on average 63% larger: pLS403 HIS3 pLS404 TRP1 pLS405 LEU2 pLS406 URA3 pLS408 pLS410 ). These vector backbones new opportunities efficient molecular biology

Язык: Английский

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Construction and Characterization of Light-Responsive Transcriptional Systems

Methods in molecular biology, Год журнала: 2024, Номер unknown, С. 261 - 275

Опубликована: Янв. 1, 2024

Язык: Английский

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