Neural circuit mechanisms for steering control in walking Drosophila DOI Open Access

Aleksandr Rayshubskiy,

Stephen L. Holtz,

Alexander Shakeel Bates

и другие.

Опубликована: Ноя. 27, 2024

Orienting behaviors provide a continuous stream of information about an organism’s sensory experiences and plans. Thus, to study the links between sensation action, it is useful identify neurons in brain that control orienting behaviors. Here we describe descending Drosophila predict influence orientation (heading) during walking. We show these cells have specialized functions: whereas one cell type predicts sustained low-gain steering, other transient high-gain steering. These latter integrate internally-directed steering signals from head direction system with stimulus-directed multimodal pathways. The inputs are organized produce “see-saw” commands, so increasing output hemisphere accompanied by decreasing hemisphere. Together, our results internal external drives integrated motor commands different timescales, for flexible precise space.

Язык: Английский

Neural circuit mechanisms for steering control in walkingDrosophila DOI Creative Commons

Aleksandr Rayshubskiy,

Stephen L. Holtz,

Alexander Shakeel Bates

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2020, Номер unknown

Опубликована: Апрель 5, 2020

Abstract Orienting behaviors provide a continuous stream of information about an organism’s sensory experiences and plans. Thus, to study the links between sensation action, it is useful identify neurons in brain that control orienting behaviors. Here we describe descending Drosophila predict influence orientation (heading) during walking. We show these cells have specialized functions: whereas one cell type predicts sustained low-gain steering, other transient high-gain steering. These latter integrate internally-directed steering signals from head direction system with stimulus-directed multimodal pathways. The inputs are organized produce “see-saw” commands, so increasing output hemisphere accompanied by decreasing hemisphere. Together, our results internal external drives integrated motor commands different timescales, for flexible precise space.

Язык: Английский

Процитировано

72
Neural circuit mechanisms for steering control in walking Drosophila DOI Open Access

Aleksandr Rayshubskiy,

Stephen L. Holtz,

Alexander Shakeel Bates

и другие.

Опубликована: Ноя. 27, 2024

Orienting behaviors provide a continuous stream of information about an organism’s sensory experiences and plans. Thus, to study the links between sensation action, it is useful identify neurons in brain that control orienting behaviors. Here we describe descending Drosophila predict influence orientation (heading) during walking. We show these cells have specialized functions: whereas one cell type predicts sustained low-gain steering, other transient high-gain steering. These latter integrate internally-directed steering signals from head direction system with stimulus-directed multimodal pathways. The inputs are organized produce “see-saw” commands, so increasing output hemisphere accompanied by decreasing hemisphere. Together, our results internal external drives integrated motor commands different timescales, for flexible precise space.

Язык: Английский

Процитировано

13
A split-GAL4 driver line resource forDrosophilaneuron types DOI Creative Commons
Geoffrey W. Meissner,

Allison Vannan,

Jennifer Jeter

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Янв. 10, 2024

Abstract Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. Previously published new included, all validated for expression curated optimal type specificity across diverse types. In addition stocks well-characterized lines, we make available 300,000 3D lines.

Язык: Английский

Процитировано

12
eLife assessment: A split-GAL4 driver line resource for Drosophila CNS cell types DOI Open Access
Tânia Reis

Опубликована: Июль 30, 2024

Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. In addition stocks for well-characterized lines, we make available 300,000 new 3D lines.

Язык: Английский

Процитировано

4
A split-GAL4 driver line resource for Drosophila neuron types DOI Open Access
Geoffrey W. Meissner,

Allison Vannan,

Jennifer Jeter

и другие.

Опубликована: Янв. 6, 2025

Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. Previously published new included, all validated for expression curated optimal type specificity across diverse types. In addition stocks well-characterized lines, we make available 300,000 3D lines.

Язык: Английский

Процитировано

0
A split-GAL4 driver line resource for Drosophila neuron types DOI Creative Commons
Geoffrey W. Meissner,

Allison Vannan,

Jennifer Jeter

и другие.

eLife, Год журнала: 2025, Номер 13

Опубликована: Янв. 24, 2025

Techniques that enable precise manipulations of subsets neurons in the fly central nervous system (CNS) have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult CNS 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. Previously published new included, all validated for expression curated optimal cell-type specificity across diverse types. In addition stocks well-characterized lines, we make available 300,000 3D lines.

Язык: Английский

Процитировано

0
A split-GAL4 driver line resource for Drosophila CNS cell types DOI Open Access
Geoffrey W. Meissner,

Allison Vannan,

Jennifer Jeter

и другие.

Опубликована: Июль 30, 2024

Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. In addition stocks for well-characterized lines, we make available 300,000 new 3D lines.

Язык: Английский

Процитировано

3
Author response: A split-GAL4 driver line resource for Drosophila CNS cell types DOI Open Access
Geoffrey W. Meissner,

Allison Vannan,

Jennifer Jeter

и другие.

Опубликована: Июль 30, 2024

Techniques that enable precise manipulations of subsets neurons in the fly central nervous system have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. In addition stocks for well-characterized lines, we make available 300,000 new 3D lines.

Язык: Английский

Процитировано

1
The Fly Disco: Hardware and software for optogenetics and fine-grained fly behavior analysis DOI Creative Commons
Alice A. Robie, Adam L. Taylor, Catherine E. Schretter

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 4, 2024

Abstract In the fruit fly, Drosophila melanogaster , connectome data and genetic tools provide a unique opportunity to study complex behaviors including navigation, mating, aggression, grooming in an organism with tractable nervous system of 140,000 neurons. Here we present Fly Disco, flexible for high quality video collection, optogenetic manipulation, fine-grained behavioral analysis freely walking socializing fly groups. The collection hardware software automates videos synced programmable stimuli. Key pipeline features include based on trajectories 21 keypoints optogenetic-specific summary statistics visualization. We created multifly dataset pose estimation that includes 9701 examples enriched behaviors. All designs, software, are available.

Язык: Английский

Процитировано

1
A split-GAL4 driver line resource for Drosophila CNS cell types DOI Creative Commons
Geoffrey W. Meissner,

Allison Vannan,

Jennifer Jeter

и другие.

eLife, Год журнала: 2024, Номер 13

Опубликована: Июль 30, 2024

Techniques that enable precise manipulations of subsets neurons in the fly central nervous system (CNS) have greatly facilitated our understanding neural basis behavior. Split-GAL4 driver lines allow specific targeting cell types Drosophila melanogaster and other species. We describe here a collection 3060 range adult CNS 1373 characterized third-instar larvae. These tools functional, transcriptomic, proteomic studies based on anatomical targeting. NeuronBridge search relate light microscopy images these split-GAL4 to connectomes reconstructed from electron images. The collections are result screening over 77,000 split hemidriver combinations. Previously published new included, all validated for expression curated optimal cell-type specificity across diverse types. In addition stocks well-characterized lines, we make available 300,000 3D lines.

Язык: Английский

Процитировано

0