Biophysical Reviews, Год журнала: 2024, Номер 16(6), С. 833 - 849
Опубликована: Окт. 23, 2024
Cells and tissues are often under some level of confinement, imposed by the microenvironment neighboring cells, meaning that there limitations to cell size, volume changes, fluid exchanges. 3D culture, increasingly used for both single cells organoids, inherently impose levels confinement absent in 2D systems. It is thus key understand how different influences survival, function, fate. well known mechanical properties microenvironment, such as stiffness stress relaxation, important activating mechanosensitive pathways, these responsive conditions. In this review, we look at low, intermediate, high modulate activation mechanobiology tumor spheroids, with a specific focus on microwells, elastic, or viscoelastic scaffolds. addition, confining can drastically limit cellular communication healthy diseased tissues, due extracellular crowding. We discuss potential implications crowding molecular transport, matrix deposition, transport. Understanding sense respond various should inform design engineered matrices recapitulate physical tissues.
Язык: Английский