Clonal Interference and Changing Selective Pressures Shape the Escape of Sars-Cov-2 from Hundreds of Antibodies
Опубликована: Янв. 1, 2025
Язык: Английский
Deep mutational scanning of rabies glycoprotein defines mutational constraint and antibody-escape mutations
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 17, 2024
Abstract
Rabies
virus
causes
nearly
60,000
human
deaths
annually.
Antibodies
that
target
the
rabies
glycoprotein
(G)
are
being
developed
as
post-exposure
prophylactics,
but
mutations
in
G
can
render
such
antibodies
ineffective.
Here,
we
use
pseudovirus
deep
mutational
scanning
to
measure
how
all
single
amino-acid
affect
cell
entry
and
neutralization
by
a
panel
of
antibodies.
These
measurements
identify
sites
critical
for
G’s
function,
define
constrained
regions
attractive
epitopes
clinical
antibodies,
including
at
apex
base
protein.
We
provide
complete
maps
escape
eight
monoclonal
some
or
development.
Escape
most
present
natural
strains.
Overall,
this
work
provides
comprehensive
information
on
functional
antigenic
effects
help
inform
development
stabilized
vaccine
antigens
resilient
genetic
variation.
Язык: Английский
A mRNA Vaccine Encoding for a 60-mer G Glycoprotein Nanoparticle Elicits a Robust Neutralizing Antibodies Response Against the Nipah Virus
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 5, 2024
The
Nipah
virus
is
a
zoonotic
pathogen
causing
encephalitis
and
acute
respiratory
illness
in
humans
with
very
high
fatality
rates.
Here
we
report
novel
messenger
RNA
vaccine
that
directly
encodes
for
nanoparticle
displaying
60
head
domains
of
the
G
(NiV
G)
glycoprotein
acts
as
highly
effective
antigen.
A
encoding
NiV
elicits
antibody
titers
against
robust
neutralizing
response
pseudotyped
system.
We
ultimately
find
proposed
mRNA
(mRNA
G-NP)
provides
flexible
platform
development
vaccines
will
likely
be
great
value
combatting
future
outbreaks.
Язык: Английский