rRNA-specific antisense DNA and dsDNA trigger rRNA biogenesis and cause potent insecticidal effect on insect pestCoccus hesperidumL. DOI Creative Commons
Volodymyr V. Oberemok, Nikita V. Gal’chinsky, Ilya Novikov

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 17, 2024

Abstract As a completely new principle and active substance for plant protection, unmodified DNA was shown to function as contact insecticide in 2008. CUAD (contact antisense DNA) biotechnology is built on the use of short oligonucleotides insect pest control. Being novel class insecticides, oligonucleotide insecticides target rRNAs and/or pre-rRNAs recently showed high insecticidal potential against sap-feeding pests, main vectors viruses one most economically-damaging groups herbivorous insects. In order all possible opportunities biotechnology, this article we investigated 11-mer oligos comparison with long 56-mer single-stranded double-stranded sequences Coccus hesperidum control found lower efficiency latter. Also show that containment (DNAc) mechanism, sternorrhynchans, represents interesting important cell life interplay between different types oligos. course DNAc, hypercompensation rRNA triggered by highly somewhat complementary but more pronounced later degradation significant mortality seen only case perfect complementarity rRNA. Oligonucleotide are effective safe agents sternorrhynchans other easy ‘tune’ particular crops also flexible instantly re-create target-site resistance. Minimalist approach, dissolved water, so potent selective eco-friendly innovation reveals entirely dimension protection – DNA-programmable Fundamentally important, surprising results reveal regulation expression via interaction viral DNA. Significance Statement Using platform any farmer, botanical garden or company capable creating its own unique arsenal well-tailored population pest. Most technological innovations start out very expensive don’t work well, eventually working well become cheap. close point be implemented at affordable price certain pests large scale. Results provide knowledge about host sheds light

Язык: Английский

rRNA-specific antisense DNA and dsDNA trigger rRNA biogenesis and cause potent insecticidal effect on insect pestCoccus hesperidumL. DOI Creative Commons
Volodymyr V. Oberemok, Nikita V. Gal’chinsky, Ilya Novikov

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 17, 2024

Abstract As a completely new principle and active substance for plant protection, unmodified DNA was shown to function as contact insecticide in 2008. CUAD (contact antisense DNA) biotechnology is built on the use of short oligonucleotides insect pest control. Being novel class insecticides, oligonucleotide insecticides target rRNAs and/or pre-rRNAs recently showed high insecticidal potential against sap-feeding pests, main vectors viruses one most economically-damaging groups herbivorous insects. In order all possible opportunities biotechnology, this article we investigated 11-mer oligos comparison with long 56-mer single-stranded double-stranded sequences Coccus hesperidum control found lower efficiency latter. Also show that containment (DNAc) mechanism, sternorrhynchans, represents interesting important cell life interplay between different types oligos. course DNAc, hypercompensation rRNA triggered by highly somewhat complementary but more pronounced later degradation significant mortality seen only case perfect complementarity rRNA. Oligonucleotide are effective safe agents sternorrhynchans other easy ‘tune’ particular crops also flexible instantly re-create target-site resistance. Minimalist approach, dissolved water, so potent selective eco-friendly innovation reveals entirely dimension protection – DNA-programmable Fundamentally important, surprising results reveal regulation expression via interaction viral DNA. Significance Statement Using platform any farmer, botanical garden or company capable creating its own unique arsenal well-tailored population pest. Most technological innovations start out very expensive don’t work well, eventually working well become cheap. close point be implemented at affordable price certain pests large scale. Results provide knowledge about host sheds light

Язык: Английский

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