Pre-pandemic cross-reactive antibody and cellular responses against SARS-CoV-2 among female sex workers in Dakar, Senegal DOI Creative Commons
Bobby Brooke Herrera, Beth Chaplin, Souleymane Mboup

и другие.

Frontiers in Public Health, Год журнала: 2025, Номер 13

Опубликована: Янв. 23, 2025

Background The COVID-19 pandemic had a severe impact globally, yet African populations exhibited unexpectedly lower rates of disease and mortality. We investigated the potential role pre-existing immunity in shaping epidemiology Africa. Methods Plasma collected from Senegalese female sex workers prior to was screened for SARS-CoV-2 human coronavirus (hCoV) antibodies by virion immunoblots. For antibody-reactive plasma, paired peripheral blood mononuclear cells were stimulated fusion proteins IFN- γ cellular responses assessed via ELISPOT. Results observed substantial levels cross-reactive SARS-CoV-2, stemming exposure seasonal hCoVs. Our antibody analysis revealed 23.5% (47/200) seroprevalence rate against nucleocapsid (N). These samples then probed hCoV spike (S) and/or N antigens; 85.1% (40/47), 70.2% (33/47), 95.7% (45/47) reactive hCoV-229E, hCoV-OC43, or hCoV-HKU1, respectively. also demonstrated cross-reactivity with 80.0% (36/45) 82.2% (37/45) showing S N, A unique pre-pandemic subject detectable neutralization responses. Conclusion findings suggest that may induce adaptive immunity, potentially contributing protection COVID-19. study provides data on dynamics Senegal underscores importance understanding outcomes globally.

Язык: Английский

Pre-pandemic cross-reactive antibody and cellular responses against SARS-CoV-2 among female sex workers in Dakar, Senegal DOI Creative Commons
Bobby Brooke Herrera, Beth Chaplin, Souleymane Mboup

и другие.

Frontiers in Public Health, Год журнала: 2025, Номер 13

Опубликована: Янв. 23, 2025

Background The COVID-19 pandemic had a severe impact globally, yet African populations exhibited unexpectedly lower rates of disease and mortality. We investigated the potential role pre-existing immunity in shaping epidemiology Africa. Methods Plasma collected from Senegalese female sex workers prior to was screened for SARS-CoV-2 human coronavirus (hCoV) antibodies by virion immunoblots. For antibody-reactive plasma, paired peripheral blood mononuclear cells were stimulated fusion proteins IFN- γ cellular responses assessed via ELISPOT. Results observed substantial levels cross-reactive SARS-CoV-2, stemming exposure seasonal hCoVs. Our antibody analysis revealed 23.5% (47/200) seroprevalence rate against nucleocapsid (N). These samples then probed hCoV spike (S) and/or N antigens; 85.1% (40/47), 70.2% (33/47), 95.7% (45/47) reactive hCoV-229E, hCoV-OC43, or hCoV-HKU1, respectively. also demonstrated cross-reactivity with 80.0% (36/45) 82.2% (37/45) showing S N, A unique pre-pandemic subject detectable neutralization responses. Conclusion findings suggest that may induce adaptive immunity, potentially contributing protection COVID-19. study provides data on dynamics Senegal underscores importance understanding outcomes globally.

Язык: Английский

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