bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 6, 2024

Abstract The extracellular matrix (ECM) is a dynamic network structure that surrounds, supports, and influences cell behaviour. It facilitates communication plays an important role in functions such as growth migration. One way cells interact with the ECM via focal adhesions, which enable them to sense respond mechanical properties exert traction forces deform it. This interplay between ECM, many aspects of remain incompletely understood, involves coordination processes acting at different spatial scales highly influenced by cells, adhesion components. To gain better understanding these interactions, we have developed multiscale agent-based model based on description simultaneously integrates mechanosensitive regulation cytoskeleton dynamics, deformation. We use our quantify cell-cell mediated deformation show how this process depends fibres topology network. In particular, analyse influence stiffness contraction activity transmission cues distinct timescales associated cell-ECM interaction. Our simulations predict increased for stronger sweet spot along its fibres. also affects ability stiffer ECMs transmit it can induce detachment from ECM. Finally, demonstrate integrating across temporal crucial Author summary surrounding fibrous known (ECM). supports behaviour, playing key communication, growth, Cells ECM’s it, leading stress. These changes are transmitted fibres, influencing behaviour neighbouring cells. Different subcellular structures components various scales, making mathematical modelling valuable tool analysing interactions. force-based quantifies stress transmission, captures detachment, explores impact both analysis shows increases suggests range effective communication. investigate modifying stiff when subject cell-induced forces. results importance coupling occurring capture overall

Язык: Английский