Divergence in cellular markers observed in single-cell transcriptomics datasets between cultured primary trabecular meshwork cells and tissues
Scientific Data,
Год журнала:
2025,
Номер
12(1)
Опубликована: Фев. 14, 2025
Abstract
The
trabecular
meshwork
within
the
outflow
apparatus
is
critical
in
maintaining
intraocular
pressure
homeostasis.
In
vitro
studies
employing
primary
cell
cultures
of
human
(hTM)
have
conventionally
served
as
surrogates
for
investigating
pathobiology
TM
dysfunction.
Despite
its
abundant
use,
translation
outcomes
from
to
ex
vivo
and/or
remains
a
challenge.
Given
heterogeneity,
performing
single-cell
RNA
sequencing
comparing
hTM
tissue
may
provide
important
insights
on
cellular
identity
and
translatability,
such
an
approach
has
not
been
reported
before.
this
study,
we
assembled
total
14
samples
across
passages
1–4,
including
4
individuals
diagnosed
with
glaucoma.
This
dataset
offers
comprehensive
transcriptomic
resource
scRNA-seq
data
study
global
changes
gene
expression
comparison
cells
situ
.
We
performed
extensive
preprocessing
quality
control,
allowing
research
community
access
utilize
public
resource.
Язык: Английский
Mechanical Forces, Nucleus, Chromosomes, and Chromatin
Biomolecules,
Год журнала:
2025,
Номер
15(3), С. 354 - 354
Опубликована: Март 1, 2025
Individual
cells
and
within
the
tissues
organs
constantly
face
mechanical
challenges,
such
as
tension,
compression,
strain,
shear
stress,
rigidity
of
cellular
extracellular
surroundings.
Besides
external
forces,
their
components
are
also
subjected
to
intracellular
pulling,
pushing,
stretching,
created
by
sophisticated
force-generation
machinery
cytoskeleton
molecular
motors.
All
these
stressors
switch
on
mechanotransduction
pathways,
allowing
respond
adapt.
Mechanical
force-induced
changes
at
cell
membrane
transmitted
nucleus
its
nucleoskeleton,
affecting
nucleocytoplasmic
transport,
chromatin
conformation,
transcriptional
activity,
replication,
genome,
which,
in
turn,
orchestrate
behavior.
The
memory
mechanoresponses
is
stored
epigenetic
structure
modifications.
state
response
acellular
environment
determines
identity,
fate,
immune
invading
pathogens.
Here,
we
give
a
short
overview
latest
developments
understanding
processes,
emphasizing
effects
nuclei,
chromosomes,
chromatin.
Язык: Английский
Modeling how lamellipodia-driven cells maintain persistent migration and interact with external barriers
Physical Review Research,
Год журнала:
2025,
Номер
7(1)
Опубликована: Март 27, 2025
Cell
motility
is
fundamental
to
many
biological
processes,
and
cells
exhibit
a
variety
of
migration
patterns.
Many
motile
cell
types
follow
universal
law
that
connects
their
speed
persistency,
property
can
originate
from
the
intracellular
transport
polarity
cues
due
global
actin
retrograde
flow.
This
mechanism
was
termed
“Universal
Coupling
between
Speed
Persistency”(UCSP).
Here
we
implement
simplified
version
UCSP
in
coarse-grained
“minimal-cell”
model,
which
composed
three-dimensional
vesicle
contains
curved
active
proteins.
model
spontaneously
forms
lamellipodia-like
shape,
is,
however,
sensitive
depolarize
into
nonmotile
form
random
fluctuations
or
when
interacting
with
external
obstacles.
The
implementation
introduces
long-range
inhibition,
stabilizes
phenotype.
allows
our
describe
robust
observed
explain
large
cellular
dynamics,
such
as
relation
aspect
ratio,
cell-barrier
scattering,
oscillations
different
geometric
confinements.
Published
by
American
Physical
Society
2025
Язык: Английский
Amino-Functionalized Mesoporous Silica Film as a Spatiotemporally Matched Degradable Nanotopography to Enhance Early Bioactivity and Osteogenesis on Titania Nanotube Surfaces
ACS Applied Materials & Interfaces,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 31, 2025
Nanotopographic
fabrication
has
been
proven
to
enhance
the
osteoinductivity
of
titanium
implant
surfaces;
however,
it
is
difficult
for
static
nanostructures
regulate
multiple
osteoblast
behaviors.
Herein,
we
proposed
a
novel
strategy
further
modifying
nanostructured
surfaces
using
degradable
nanotopography
that
was
beneficial
specific
cellular
processes
and
spatiotemporally
matched.
In
this
work,
titania
nanotube
(TNT)
array,
known
its
strong
capability
promote
osteogenic
differentiation,
employed
as
substrate.
An
oil-water
biphase
system
containing
3-aminopropyl
triethoxysilane
(APTES)
tetraethyl
orthosilicate
(TEOS)
utilized
achieve
in
situ
deposition
amino-functionalized
mesoporous
silica
films
on
TNT
surface
(TNT@AHMS).
The
numerous
mesopores
(∼4
nm)
amino
groups
AHMS
significantly
improved
protein
adsorption
attachment
rat
bone
marrow
mesenchymal
stem
cells
(rBMSCs).
By
culturing
an
AHMS-conditioned
medium,
effects
enhancing
early
cell
behavior
were
observed
initially
attributed
potential
synergism
topography
silicon
element
release
(∼18
ppm).
Impressively,
effect
maintained
even
when
reseeded
normal
culture
substrates.
After
24
h,
degraded
completely,
degradation
products
facilitated
subsequent
re-exposed
TNT,
which
accounted
robust
osteogenesis
both
vitro
vivo.
This
study
demonstrated
can
serve
(like
buffer
layer)
accelerate
adhesion
matched
manner,
resulting
enhanced
bioactivity
well-designed
underlying
without
influencing
physicochemical
properties.
Язык: Английский
Modelling how lamellipodia-driven cells maintain persistent migration and interact with external barriers
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 7, 2024
Cell
motility
is
fundamental
to
many
biological
processes,
and
cells
exhibit
a
variety
of
migration
patterns.
Many
motile
cell
types
follow
universal
law
that
connects
their
speed
persistency,
property
can
originate
from
the
intracellular
transport
polarity
cues
due
global
actin
retrograde
flow.
This
mechanism
was
termed
“Universal
Coupling
between
Speed
Persistency”(UCSP).
Here
we
implemented
simplified
version
UCSP
in
coarse-grained
“minimal-cell”
model,
which
composed
three-dimensional
vesicle
contains
curved
active
proteins.
model
spontaneously
forms
lamellipodia-like
shape,
however
sensitive
depolarize
into
non-motile
form
random
fluctuations
or
when
interacting
with
external
obstacles.
The
implementation
introduces
long-range
inhibition,
stabilizes
phenotype.
allows
our
describe
robust
observed
explain
large
cellular
dynamics,
such
as
relation
aspect
ratio,
cell-barrier
scattering,
oscillations
different
geometric
confinements.
Significance
Statement
membrane
proteins
protrusive
forces
arise
recruited
polymerization,
lead,
presence
adhesion,
self-organization
leading-edge
cluster
“minimal-cell”.
However,
this
polarized
shape
become
unstable,
interactions
perturbations
transform
an
immotile,
symmetric
shape.
couple
spatial
organization
advection
cue
along
cell’s
activity
axis.
Introducing
resultant
gradient
polarity-cue
motile,
vesicle.
We
thereby
present
shape-migration
relations,
scattering
spontaneous
confined
cells.
Язык: Английский