Cocaine taking and craving produce distinct transcriptional profiles in dopamine neurons DOI Creative Commons
Tate A. Pollock, Alexander V. Margetts, Samara J. Vilca

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 12, 2024

Dopamine (DA) signaling plays an essential role in reward valence attribution and encoding the reinforcing properties of natural artificial rewards. The adaptive responses from midbrain dopamine neurons to rewards such as drugs abuse are therefore important for understanding development substance use disorders. Drug-induced changes gene expression one adaptation that can determine activity projection regions brain system. One major challenges obtaining this involves complex cellular makeup brain, where each neuron population be defined by a distinct transcriptional profile. To bridge gap, we have adapted virus-based method labeling capture nuclei, coupled with nuclear RNA-sequencing, study adaptations, specifically, ventral tegmental area (VTA) during cocaine taking craving, using mouse model intravenous self-administration (IVSA). Our results show significant across non-drug operant training, taking, highlighted enrichment repressive epigenetic modifying enzyme craving. Immunohistochemical validation further revealed increase H3K9me3 deposition DA These demonstrate cocaine-induced adaptations vary phase underscore utility approach identifying relevant phase-specific molecular targets behavioral course

Язык: Английский

Cocaine taking and craving produce distinct transcriptional profiles in dopamine neurons DOI Creative Commons
Tate A. Pollock, Alexander V. Margetts, Samara J. Vilca

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 12, 2024

Dopamine (DA) signaling plays an essential role in reward valence attribution and encoding the reinforcing properties of natural artificial rewards. The adaptive responses from midbrain dopamine neurons to rewards such as drugs abuse are therefore important for understanding development substance use disorders. Drug-induced changes gene expression one adaptation that can determine activity projection regions brain system. One major challenges obtaining this involves complex cellular makeup brain, where each neuron population be defined by a distinct transcriptional profile. To bridge gap, we have adapted virus-based method labeling capture nuclei, coupled with nuclear RNA-sequencing, study adaptations, specifically, ventral tegmental area (VTA) during cocaine taking craving, using mouse model intravenous self-administration (IVSA). Our results show significant across non-drug operant training, taking, highlighted enrichment repressive epigenetic modifying enzyme craving. Immunohistochemical validation further revealed increase H3K9me3 deposition DA These demonstrate cocaine-induced adaptations vary phase underscore utility approach identifying relevant phase-specific molecular targets behavioral course

Язык: Английский

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