An endogenous retroviral element co-opts an upstream regulatory sequence to achieve somatic expression and mobility DOI Creative Commons
Natalia Rubanova, Darshika Singh,

Louis Barolle

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 2, 2025

Retrotransposons, multi-copy sequences that propagate via copy-and-paste mechanisms involving an RNA intermediate, occupy large portions of all eukaryotic genomes. A great majority their manifold copies remain silenced in somatic cells, nevertheless, some are transcribed, often a tissue specific manner, and small fraction retains its ability to mobilize. Retrotransposon expression or mobility increasingly recognized contribute normal development homeostasis, as well aging disease. While it is characterized retrotransposon may provide cis regulatory elements for neighboring genes, how own achieved different contexts not understood. Here, using long-read DNA sequencing, we characterize retrotransposition the Drosophila intestine. We show retroelement does change significantly upon limited very few active sub-families retrotransposons. Importantly, identify polymorphic donor locus endogenous LTR retroviral element rover , intestinal tissue. reveal gut activity copy depends on genomic environment. Without affecting local gene expression, co-opts upstream enhancer sequence, rich transcription factor binding sites, expression. Further escargot, snail-type critical progenitor cell function, can drive transcriptional copy. These data new insights into locus-specific features allow retrotransposons produce functional transcripts mobilize lineage.

Язык: Английский

An endogenous retroviral element co-opts an upstream regulatory sequence to achieve somatic expression and mobility DOI Creative Commons
Natalia Rubanova, Darshika Singh,

Louis Barolle

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 2, 2025

Retrotransposons, multi-copy sequences that propagate via copy-and-paste mechanisms involving an RNA intermediate, occupy large portions of all eukaryotic genomes. A great majority their manifold copies remain silenced in somatic cells, nevertheless, some are transcribed, often a tissue specific manner, and small fraction retains its ability to mobilize. Retrotransposon expression or mobility increasingly recognized contribute normal development homeostasis, as well aging disease. While it is characterized retrotransposon may provide cis regulatory elements for neighboring genes, how own achieved different contexts not understood. Here, using long-read DNA sequencing, we characterize retrotransposition the Drosophila intestine. We show retroelement does change significantly upon limited very few active sub-families retrotransposons. Importantly, identify polymorphic donor locus endogenous LTR retroviral element rover , intestinal tissue. reveal gut activity copy depends on genomic environment. Without affecting local gene expression, co-opts upstream enhancer sequence, rich transcription factor binding sites, expression. Further escargot, snail-type critical progenitor cell function, can drive transcriptional copy. These data new insights into locus-specific features allow retrotransposons produce functional transcripts mobilize lineage.

Язык: Английский

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