ACS Environmental Au, Год журнала: 2025, Номер unknown
Опубликована: Янв. 22, 2025
Many polycyclic aromatic hydrocarbons (PAHs) in the environment resulting from crude oil spills and incomplete combustion of organic matter are highly toxic, mutagenic, or carcinogenic to microorganisms humans. Bioremediation PAHs using that encode biodegradative genes is a promising approach for environmental PAH cleanup. However, viability exogenous often limited due competition with native microbial community. Instead relying on survival one few species bacteria, genetic bioaugmentation harnesses conjugative plasmids spread functional microbes. In this study, two plasmid backbones differ copy number regulation, replication, mobilization were engineered contain dioxygenase gene (bphC) conjugated soil bacteria including Bacillus subtilis, Pseudomonas putida, Acinetobacter sp., as well synthetic community assembled these bacteria. Fitness effects transconjugants significantly impacted rates transfer biotransformation model (2,3-dihydroxybiphenyl). A synergistic effect was observed which communities bioaugmented bphC had higher degradation than grown monocultures. Finally, conjugation associated relative abundances communities, underscoring how fitness impacts can shape structure function.
Язык: Английский