A direct interaction between the Chd1 CHCT domain and Rtf1 controls Chd1 distribution and nucleosome positioning on active genes DOI Creative Commons
Sarah A. Tripplehorn, Margaret K. Shirra, Santana M. Lardo

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 6, 2024

ABSTRACT The nucleosome remodeler Chd1 is required for the re-establishment of positioning in wake transcription elongation by RNA Polymerase II. Previously, we found that occupancy on gene bodies depends Rtf1 subunit Paf1 complex yeast. Here, identify an N-terminal region and CHCT domain as sufficient their interaction demonstrate this direct. Mutations disrupt Rtf1-Chd1 result accumulation at 5’ ends Chd1-occupied genes, increased cryptic transcription, altered positioning, concordant shifts histone modification profiles. We show a homologous within mouse RTF1 interacts with domains CHD1 CHD2. This work supports conserved mechanism coupling family proteins to identifies cellular function about which little known.

Язык: Английский

A direct interaction between the Chd1 CHCT domain and Rtf1 controls Chd1 distribution and nucleosome positioning on active genes DOI Creative Commons
Sarah A. Tripplehorn, Margaret K. Shirra, Santana M. Lardo

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 6, 2024

ABSTRACT The nucleosome remodeler Chd1 is required for the re-establishment of positioning in wake transcription elongation by RNA Polymerase II. Previously, we found that occupancy on gene bodies depends Rtf1 subunit Paf1 complex yeast. Here, identify an N-terminal region and CHCT domain as sufficient their interaction demonstrate this direct. Mutations disrupt Rtf1-Chd1 result accumulation at 5’ ends Chd1-occupied genes, increased cryptic transcription, altered positioning, concordant shifts histone modification profiles. We show a homologous within mouse RTF1 interacts with domains CHD1 CHD2. This work supports conserved mechanism coupling family proteins to identifies cellular function about which little known.

Язык: Английский

Процитировано

1