bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown
Опубликована: Дек. 30, 2023
Neuronal firing sequences are thought to be the basic building blocks of neural coding and information broadcasting within brain. However, when emerge during neurodevelopment remains unknown. We demonstrate that structured present in spontaneous activity human brain organoids
Язык: Английский
Current Opinion in Genetics & Development, Год журнала: 2025, Номер 91, С. 102324 - 102324
Опубликована: Фев. 20, 2025
Язык: Английский
Процитировано
0
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown
Опубликована: Март 2, 2025
ABSTRACT Improving models to investigate neurodegenerative disease, neurodevelopmental neuro-toxicology and neuropharmacology is critical improve our basic understanding of the human nervous system, as well accelerate discovery interventions drugs. Improved central system could enable discoveries related functional changes induced by sensory stimulation or toxic exposures. Neural organoids, complex three-dimensional cell cultures derived from adult stem cells, have been grown with connectivity neuroanatomy. Moreover, these interfaced bi-directional electrical recording, chemical stimulation. This effort sought develop new computational techniques which be applied comparative studies using neural organoids. In particular, we adapted CEBRA, a state art model in vivo modeling literature, generate 2D 3D embeddings (projections into structured low-dimensional spaces) high dimensional organoid electrophysiology data. can done an unsupervised semi-supervised manner. Results indicate quickly reliably generated serve low-dimensional, interpretable for characterizing activity over time, clustering results around known bursting phenomena. demonstrate that mixtures von Mises-Fisher distributions used parametric embedding spaces statistics hypothesis testing. technique may types creating representation quantitative comparison validation against animal Looking ahead, this work allow formulation class experiments investigating impact toxins, genetic manipulations, pharmacological on neurons.
Язык: Английский
Процитировано
0
The Neuroscientist, Год журнала: 2025, Номер unknown
Опубликована: Март 13, 2025
Harnessing intelligence from brain cells in vitro requires a multidisciplinary approach integrating wetware, hardware, and software. Wetware comprises the themselves, where differentiation induced pluripotent stem offers ethical scalability; hardware typically involves life support system setup to record activity deliver stimulation cells; software is required control process signals coming going cells. This review provides broad summary of foundational technologies underpinning these components, along with outlining importance technology integration. Of particular that this new ability extend beyond traditional methods assess primarily survival spontaneous neural cultures. Instead, focus returns core function tissue: neurocomputational information respond accordingly. Therefore, also covers work that, despite relatively early state current technology, has provided novel meaningful understandings field neuroscience opening exciting avenues for future research.
Язык: Английский
Процитировано
0
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown
Опубликована: Дек. 30, 2023
Neuronal firing sequences are thought to be the basic building blocks of neural coding and information broadcasting within brain. However, when emerge during neurodevelopment remains unknown. We demonstrate that structured present in spontaneous activity human brain organoids
Язык: Английский
Процитировано
6