The spinal premotor network driving scratching flexor and extensor alternation DOI Creative Commons

Miaojie Yao,

Akira Nagamori, Eiman Azim

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 8, 2025

Rhythmic motor behaviors are generated by neural networks termed central pattern generators (CPGs). Although locomotor CPGs have been extensively characterized, it remains unknown how the neuronal populations composing them interact to generate adaptive rhythms. We explored non-linear cooperation dynamics among three main of ipsilaterally projecting spinal CPG neurons - V1, V2a, V2b in scratch reflex rhythmogenesis. Ablation all subtypes reduced oscillation frequency. Activation excitatory V2a enhanced frequency, while activating inhibitory V1 caused atonia. These findings required development a novel neuromechanical model that consists flexor and extensor modules coupled via inhibition, which rhythm each module is self-bursting accelerated intra-module inhibition. Inter-module inhibition coordinates phases activity slows oscillations, facilitation mechanisms explain activation-driven increase

Язык: Английский

The transcriptomic landscape of spinal V1 interneurons reveals a role for En1 in specific elements of motor output DOI Creative Commons

Alexandra J. Trevisan,

Katie Han, Phillip Chapman

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Сен. 19, 2024

SUMMARY Neural circuits in the spinal cord are composed of diverse sets interneurons that play crucial roles shaping motor output. Despite progress revealing cellular architecture cord, extent cell type heterogeneity within interneuron populations remains unclear. Here, we present a single-nucleus transcriptomic atlas V1 across postnatal development. We find core molecular taxonomy distinguishing neonatal perdures into adulthood, suggesting conservation function Moreover, identify key role for En1, transcription factor marks population, specifying one unique subset Pou6f2 interneurons. Loss En1 selectively disrupts frequency rhythmic locomotor output but does not disrupt flexion/extension limb movement. Beyond serving as resource this neuronal our study highlights how deep profiling provides an entry point functional studies specialized types

Язык: Английский

Процитировано

4
The spinal premotor network driving scratching flexor and extensor alternation DOI Creative Commons

Miaojie Yao,

Akira Nagamori, Eiman Azim

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 8, 2025

Rhythmic motor behaviors are generated by neural networks termed central pattern generators (CPGs). Although locomotor CPGs have been extensively characterized, it remains unknown how the neuronal populations composing them interact to generate adaptive rhythms. We explored non-linear cooperation dynamics among three main of ipsilaterally projecting spinal CPG neurons - V1, V2a, V2b in scratch reflex rhythmogenesis. Ablation all subtypes reduced oscillation frequency. Activation excitatory V2a enhanced frequency, while activating inhibitory V1 caused atonia. These findings required development a novel neuromechanical model that consists flexor and extensor modules coupled via inhibition, which rhythm each module is self-bursting accelerated intra-module inhibition. Inter-module inhibition coordinates phases activity slows oscillations, facilitation mechanisms explain activation-driven increase

Язык: Английский

Процитировано

0