Second Messengers

Cold Spring Harbor Perspectives in Biology, Год журнала: 2016, Номер 8(8), С. a005926 - a005926

Опубликована: Авг. 1, 2016

Alexandra C. Newton1, Martin D. Bootman2 and John Scott3 1Department of Pharmacology, University California at San Diego, La Jolla, 92093 2Department Life, Health Chemical Sciences, The Open University, Walton Hall, Milton Keynes MK7 6AA, United Kingdom 3Department Howard Hughes Medical Institute, Washington School Medicine, Seattle, 98195 Correspondence: anewton{at}ucsd.edu

Язык: Английский

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Exploring the co‐activity of FDA approved drug gemcitabine and docetaxel for enhanced anti‐breast cancer activity: DFT, docking, molecular dynamics simulation and pharmacophore studies

International Journal of Quantum Chemistry, Год журнала: 2024, Номер 124(4)

Опубликована: Фев. 13, 2024

Abstract Combining FDA‐approved medications may increase biological activity by simultaneously targeting many protein mechanisms while being less toxic. Gemcitabine and docetaxel together might have a synergistic impact that kills cancer cells makes them more effective against breast cancer. This study used the foundation set B3LYP/6–311 G to optimize gemcitabine with structure was created. Theoretical calculations were made for ultraviolet visible spectrum studied in gas liquid phase. The structural stability reactivity of combined using energy gap between HOMO LUMO, computed (ΔE) 4.219 eV. electrostatic potential complex determined Mulliken charge population evaluated. Through RDG analysis weak interactions GEDT evaluated topology properties through ELF LOL analysis. Breast target proteins utilized molecular docking studies. scores revealed greater binding affinity molecule, confirming superior combinatorial interaction docetaxel. Highest ability Caspase‐6 network assessed graph theory model. dynamic simulation 100 ns. adsorption, distribution, metabolism, excretion, toxicity characteristics investigated, findings lead compound's safety profile its use as potent anticancer medication.

Язык: Английский

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Signals and Receptors

Cold Spring Harbor Perspectives in Biology, Год журнала: 2016, Номер 8(4), С. a005900 - a005900

Опубликована: Апрель 1, 2016

Carl-Henrik Heldin1, Benson Lu2, Ron Evans2 and J. Silvio Gutkind3 1Ludwig Institute for Cancer Research, Science Life Laboratory, Uppsala University, SE-75124 Uppsala, Sweden 2The Salk Biological Studies, Gene Expression La Jolla, California 92037 3National of Dental Craniofacial National Institutes Health, Bethesda, Maryland 20892-4340 Correspondence: c-h.heldin{at}licr.uu.se

Язык: Английский

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Subversion of Cell Signaling by Pathogens

Cold Spring Harbor Perspectives in Biology, Год журнала: 2012, Номер 4(9), С. a006114 - a006114

Опубликована: Сен. 1, 2012

Neal M. Alto and Kim Orth UT Southwestern Medical Center, Dallas, Texas 75390 Correspondence: kim.orth{at}utsouthwestern.edu

Язык: Английский

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From Selenium Absorption to Selenoprotein Degradation

Biological Trace Element Research, Год журнала: 2019, Номер 192(1), С. 26 - 37

Опубликована: Июнь 20, 2019

Язык: Английский

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Multisignal control of expression of the LHCX protein family in the marine diatomPhaeodactylum tricornutum

Journal of Experimental Botany, Год журнала: 2016, Номер 67(13), С. 3939 - 3951

Опубликована: Май 25, 2016

Diatoms are phytoplanktonic organisms that grow successfully in the ocean where light conditions highly variable. Studies of molecular mechanisms acclimation marine diatom Phaeodactylum tricornutum show carotenoid de-epoxidation enzymes and LHCX1, a member light-harvesting protein family, both contribute to dissipate excess energy through non-photochemical quenching (NPQ). In this study, we investigate role other members LHCX family stress responses. Our analysis available genomic data shows presence multiple genes is conserved feature species living different ecological niches. Moreover, an levels four P. transcripts relation expression photosynthetic activity indicates LHCXs differentially regulated under intensities nutrient starvation, mostly modulating NPQ capacity. We conclude abiotic signals converge regulate content cells, providing way fine-tune harvesting photoprotection. our indicate expansion gene reflects functional diversification its which could benefit cells responding variable environments.

Язык: Английский

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Targeting Lipid Peroxidation for Cancer Treatment

Molecules, Год журнала: 2020, Номер 25(21), С. 5144 - 5144

Опубликована: Ноя. 5, 2020

Cancer is one of the highest prevalent diseases in humans. The chances surviving cancer and its prognosis are very dependent on affected tissue, body location, stage at which disease diagnosed. Researchers pharmaceutical companies worldwide pursuing many attempts to look for compounds treat this malignancy. Most current strategies fight implicate use acting DNA damage checkpoints, non-receptor tyrosine kinases activities, regulators hedgehog signaling pathways, metabolic adaptations placed cancer. In last decade, finding a lipid peroxidation increase linked 15-lipoxygenases isoform 1 (15-LOX-1) activity stimulation has been found specific successful treatments against This discovery contrasts with production other oxidation signatures generated by lipoxygenases such as 5-LOX 12-LOX, cyclooxygenase (COX-2) have suggested biomarkers inhibitors present anti-tumoral antiproliferative activities. These findings support previously proposed role hydroperoxides their metabolites cell mediators. Depletion or promotion generally related source associated tissue originates. review highlights potential therapeutical chemical derivatives stimulate block cellular routes generate disease.

Язык: Английский

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Towards the Idea of Molecular Brains

International Journal of Molecular Sciences, Год журнала: 2021, Номер 22(21), С. 11868 - 11868

Опубликована: Ноя. 1, 2021

How can single cells without nervous systems perform complex behaviours such as habituation, associative learning and decision making, which are considered the hallmark of animals with a brain? Are there molecular that underlie cognitive properties equivalent to those This review follows development idea brains from Darwin’s “root brain hypothesis”, through bacterial chemotaxis, recent discovery neuron-like r-protein networks in ribosome. By combining structural biology view Bayesian approach, this explores evolutionary labyrinth information processing across scales. Ribosomal protein open window into what were probably earliest signalling emerge before radiation three kingdoms. While ribosomal characterised by long-lasting interactions between their nodes, cell essentially based on transient interactions. As corollary, while signals propagated persistent may be ephemeral, whose constrain diffusing cytoplasm durable time, post-translational modifications proteins or second messenger synthesis. The duration nature signals, turn, implies different mechanisms for integration multiple making. Evolution then reinvented metazoans. simple display architectural functional analogies comparison could suggest scale invariance processing. At level, significant complexification eukaryotic is associated burst acquisition new conserved aromatic amino acids. Knowing residues play critical role allosteric receptors channels, observation suggests general π charged acids signal We think these findings provide basis designing future computers organic processors.

Язык: Английский

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LncRNA HOTAIR regulates glucose transporter Glut1 expression and glucose uptake in macrophages during inflammation

Scientific Reports, Год журнала: 2021, Номер 11(1)

Опубликована: Янв. 8, 2021

Abstract Inflammation plays central roles in the immune response. Inflammatory response normally requires higher energy and therefore is associated with glucose metabolism. Our recent study demonstrates that lncRNA HOTAIR key NF-kB activation, cytokine expression, inflammation. Here, we investigated if any role regulation of metabolism cells during results demonstrate LPS-induced inflammation induces expression transporter isoform 1 (Glut1) which controls uptake macrophages. Glut1 regulated via activation. Importantly, siRNA-mediated knockdown suppressed suggesting macrophage. turn increases to LPS. We also found regulates macrophages its decreases increased uptake. other upstream regulators such as PTEN HIF1α, multimodal functions Overall, our demonstrated by activating hence metabolic programming potentially meet needs

Язык: Английский

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Emerging roles of O-GlcNAcylation in protein trafficking and secretion

Journal of Biological Chemistry, Год журнала: 2024, Номер 300(3), С. 105677 - 105677

Опубликована: Янв. 23, 2024

The emerging roles of O-GlcNAcylation, a distinctive post-translational modification, are increasingly recognized for their involvement in the intricate processes protein trafficking and secretion. This modification exerts its influence on both conventional unconventional secretory pathways. Under healthy stress conditions, such as during diseases, it orchestrates transport proteins within cells, ensuring timely delivery to intended destinations. O-GlcNAcylation occurs key factors, like coat complexes (COPI COPII), clathrin, SNAREs (soluble N-ethylmaleimide-sensitive factor attachment receptors), GRASP55 (Golgi reassembly stacking 55 kDa) that control vesicle budding fusion anterograde retrograde understanding offers valuable insights into critical functions cellular physiology progression including neurodegeneration, cancer, metabolic disorders. In this review, we summarize discuss latest findings elucidating O-GlcNAc significance various human

Язык: Английский

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