International Journal of Translational Medicine,
Год журнала:
2022,
Номер
2(3), С. 419 - 447
Опубликована: Авг. 19, 2022
Despite
significant
advances
in
the
understanding
of
cancer
biology,
is
still
a
leading
cause
death
worldwide.
Expression
tumor
microenvironment
component,
osteopontin,
tissues,
plasma,
and
serum,
has
been
shown
to
be
associated
with
poor
prognosis
survival
rate
various
human
cancers.
Recent
studies
suggest
that
osteopontin
drives
development
aggressiveness
using
strategies.
In
this
review,
we
first
provide
an
overview
how
promotes
progression,
such
as
growth,
invasion,
angiogenesis,
immune
modulation,
well
metastasis
chemoresistance.
Next,
address
functional
activities
are
modulated
by
interaction
integrins
CD44
receptors,
but
also
post-translational
modification,
proteolytic
processing
several
proteases,
phosphorylation,
glycosylation.
Then,
review
activates
tumor-associated
macrophages
(TAMs)
cancer-associated
fibroblasts
(CAFs),
functions
immunosuppressor
regulating
surveillance
checkpoint
microenvironment.
Finally,
discuss
potential
applications
biomarker
therapeutic
target.
Engineered
allosteric
regulation
of
protein
activity
provides
significant
advantages
for
the
development
robust
and
broadly
applicable
tools.
However,
application
switches
in
optogenetics
has
been
scarce
suffers
from
critical
limitations.
Here,
we
report
an
optogenetic
approach
that
utilizes
engineered
Light-Regulated
(LightR)
switch
module
to
achieve
tight
spatiotemporal
control
enzymatic
activity.
Using
tyrosine
kinase
Src
as
a
model,
demonstrate
efficient
identify
temporally
distinct
signaling
responses
ranging
seconds
minutes.
LightR-Src
off-kinetics
can
be
tuned
by
modulating
LightR
photoconversion
cycle.
A
fast
cycling
variant
enables
stimulation
transient
pulses
local
selected
region
cell.
The
design
ensures
broad
applicability
tool,
achieving
light-mediated
Abl
bRaf
kinases
well
Cre
recombinase.
Nature Communications,
Год журнала:
2021,
Номер
12(1)
Опубликована: Май 4, 2021
Abstract
Haematopoietic
stem
cells
(HSCs)
tightly
regulate
their
quiescence,
proliferation,
and
differentiation
to
generate
blood
during
the
entire
lifetime.
The
mechanisms
by
which
these
critical
activities
are
balanced
still
unclear.
Here,
we
report
that
Ma
crophage-
E
rythroblast
A
ttacher
(MAEA,
also
known
as
EMP),
a
receptor
thus
far
only
identified
in
erythroblastic
island,
is
membrane-associated
E3
ubiquitin
ligase
subunit
essential
for
HSC
maintenance
lymphoid
potential.
Maea
highly
expressed
HSCs
its
deletion
mice
severely
impairs
quiescence
leads
lethal
myeloproliferative
syndrome.
Mechanistically,
have
found
surface
expression
of
several
haematopoietic
cytokine
receptors
(e.g.
MPL,
FLT3)
stabilised
absence
,
thereby
prolonging
intracellular
signalling.
This
associated
with
impaired
autophagy
flux
but
not
mature
cells.
Administration
kinase
inhibitor
or
autophagy-inducing
compounds
rescues
functional
defects
-deficient
HSCs.
Our
results
suggest
MAEA
provides
activity,
guarding
function
restricting
signalling
via
autophagy.
Frontiers in Cell and Developmental Biology,
Год журнала:
2021,
Номер
9
Опубликована: Авг. 3, 2021
The
katanin
family
of
microtubule-severing
enzymes
is
critical
for
cytoskeletal
rearrangements
that
affect
key
cellular
processes
like
division,
migration,
signaling,
and
homeostasis.
In
humans,
aberrant
expression,
or
dysfunction
the
katanins,
linked
to
developmental,
proliferative,
neurodegenerative
disorders.
Here,
we
review
current
knowledge
on
mammalian
including
an
overview
evolutionary
conservation,
functional
domain
organization,
mechanisms
regulate
activity.
We
assess
function
katanins
in
dividing
non-dividing
cells
how
their
dysregulation
promotes
impaired
ciliary
signaling
defects
developmental
programs
(corticogenesis,
gametogenesis,
neurodevelopment)
contributes
neurodegeneration
cancer.
conclude
with
perspectives
future
research
will
advance
our
understanding
this
exciting
dynamic
class
disease-associated
enzymes.
International Journal of Translational Medicine,
Год журнала:
2022,
Номер
2(3), С. 419 - 447
Опубликована: Авг. 19, 2022
Despite
significant
advances
in
the
understanding
of
cancer
biology,
is
still
a
leading
cause
death
worldwide.
Expression
tumor
microenvironment
component,
osteopontin,
tissues,
plasma,
and
serum,
has
been
shown
to
be
associated
with
poor
prognosis
survival
rate
various
human
cancers.
Recent
studies
suggest
that
osteopontin
drives
development
aggressiveness
using
strategies.
In
this
review,
we
first
provide
an
overview
how
promotes
progression,
such
as
growth,
invasion,
angiogenesis,
immune
modulation,
well
metastasis
chemoresistance.
Next,
address
functional
activities
are
modulated
by
interaction
integrins
CD44
receptors,
but
also
post-translational
modification,
proteolytic
processing
several
proteases,
phosphorylation,
glycosylation.
Then,
review
activates
tumor-associated
macrophages
(TAMs)
cancer-associated
fibroblasts
(CAFs),
functions
immunosuppressor
regulating
surveillance
checkpoint
microenvironment.
Finally,
discuss
potential
applications
biomarker
therapeutic
target.
