The molecular basis of heterochromatin assembly and epigenetic inheritance

Molecular Cell, Год журнала: 2023, Номер 83(11), С. 1767 - 1785

Опубликована: Май 18, 2023

Язык: Английский

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The sound of silence: Transgene silencing in mammalian cell engineering

Cell Systems, Год журнала: 2022, Номер 13(12), С. 950 - 973

Опубликована: Дек. 1, 2022

To elucidate principles operating in native biological systems and to develop novel biotechnologies, synthetic biology aims build integrate gene circuits within transcriptional networks. The utility of for cell engineering relies on the ability control expression all constituent transgene components. Transgene silencing, defined as loss over time, persists an obstacle primary cells stem with transgenic cargos. In this review, we highlight challenge that silencing poses robust mammalian cells, outline potential molecular mechanisms present approaches preventing silencing. We conclude a perspective identifying future research directions improving performance circuits.

Язык: Английский

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Design principles of 3D epigenetic memory systems

Science, Год журнала: 2023, Номер 382(6672)

Опубликована: Ноя. 16, 2023

Cells remember their identities, in part, by using epigenetic marks-chemical modifications placed along the genome. How can mark patterns remain stable over cell generations despite constant erosion replication and other processes? We developed a theoretical model that reveals three-dimensional (3D) genome organization stabilize memory as long (i) there is large density difference between chromatin compartments, (ii) modifying "reader-writer" enzymes spread marks three dimensions, (iii) are limited abundance relative to histone substrates. Analogous an associative encodes neuronal connectivity, encoded 3D network of chromosomal contacts. Our provides unified account diverse observations key role memory.

Язык: Английский

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Histone H3 lysine-to-methionine mutants as a paradigm to study chromatin signaling

Science, Год журнала: 2014, Номер 345(6200), С. 1065 - 1070

Опубликована: Авг. 28, 2014

Chromatin mutations disrupt development Histone proteins form the core packaging material for our genomic DNA, and covalent modifications to amino acid residues in their structure play an important role epigenetic control of gene expression. Herz et al. show that specific are normally modified regulate expression cause severe disruption normal fruit fly. Similar known be involved a subtype aggressive pediatric brain cancers. Insights into regulatory pathways disrupted by these Drosophila may suggest possible treatments human Science , this issue p. 1065

Язык: Английский

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HP1a: a structural chromosomal protein regulating transcription

Trends in Genetics, Год журнала: 2014, Номер 30(3), С. 103 - 110

Опубликована: Фев. 17, 2014

Язык: Английский

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