Cancer Cell,
Год журнала:
2021,
Номер
40(1), С. 26 - 35
Опубликована: Дек. 23, 2021
Functional
precision
medicine
is
a
strategy
whereby
live
tumor
cells
from
affected
individuals
are
directly
perturbed
with
drugs
to
provide
immediately
translatable,
personalized
information
guide
therapy.
The
heterogeneity
of
human
cancer
has
led
the
realization
that
approaches
needed
improve
treatment
outcomes.
Precision
oncology
traditionally
used
static
features
dictate
which
therapies
should
be
used.
Static
can
include
expression
key
targets
or
genomic
analysis
mutations
identify
therapeutically
targetable
"drivers."
Although
surprisingly
small
proportion
derive
clinical
benefit
approach,
functional
additional
regarding
vulnerabilities.
We
discuss
emerging
technologies
for
as
well
limitations
and
challenges
in
using
these
assays
trials
will
necessary
determine
whether
outcomes
eventually
become
standard
tool
oncology.
International Journal of Molecular Sciences,
Год журнала:
2018,
Номер
19(4), С. 1166 - 1166
Опубликована: Апрель 12, 2018
Human
malignant
tumors
are
characterized
by
pervasive
changes
in
the
patterns
of
DNA
methylation.
These
include
a
globally
hypomethylated
tumor
cell
genome
and
focal
hypermethylation
numerous
5′-cytosine-phosphate-guanine-3′
(CpG)
islands,
many
them
associated
with
gene
promoters.
It
has
been
challenging
to
link
specific
methylation
tumorigenesis
cause-and-effect
relationship.
Some
evidence
suggests
that
cancer-associated
hypomethylation
may
increase
genomic
instability.
Promoter
events
can
lead
silencing
genes
functioning
pathways
reflecting
hallmarks
cancer,
including
repair,
cycle
regulation,
promotion
apoptosis
or
control
key
tumor-relevant
signaling
networks.
A
convincing
argument
for
tumor-driving
role
be
made
when
same
also
frequently
mutated
cancer.
Many
most
commonly
hypermethylated
encode
developmental
transcription
factors,
which
permanent
silencing.
Inactivation
such
will
deprive
cells
initiate
from
option
undergoing
maintaining
lineage
differentiation
lock
into
perpetuated
stem
cell-like
state
thus
providing
an
additional
window
transformation.
Genes,
Год журнала:
2019,
Номер
10(4), С. 257 - 257
Опубликована: Март 29, 2019
DNA
methylation
(5-methylcytosine,
5mC)
is
a
major
form
of
modification
in
the
mammalian
genome
that
plays
critical
roles
chromatin
structure
and
gene
expression.
In
general,
stably
maintained
somatic
tissues.
However,
patterns
levels
show
dynamic
changes
during
development.
Specifically,
undergoes
two
waves
global
demethylation
remethylation
for
purpose
producing
next
generation.
The
first
wave
occurs
germline,
initiated
with
erasure
primordial
germ
cells
(PGCs)
completed
establishment
sex-specific
later
stages
cell
second
after
fertilization,
including
most
marks
inherited
from
gametes
subsequent
embryonic
pattern.
reprogramming
involve
both
distinct
shared
mechanisms.
this
review
article,
we
provide
an
overview
key
events,
focusing
on
important
players
these
processes,
methyltransferases
(DNMTs)
ten-eleven
translocation
(TET)
family
5mC
dioxygenases.
Theranostics,
Год журнала:
2019,
Номер
9(7), С. 2056 - 2070
Опубликована: Янв. 1, 2019
Rational:
LDCT
screening
can
identify
early-stage
lung
cancers
yet
introduces
excessive
false
positives
and
it
remains
a
great
challenge
to
differentiate
malignant
tumors
from
benign
solitary
pulmonary
nodules,
which
calls
for
better
non-invasive
diagnostic
tools.Methods:
We
performed
DNA
methylation
profiling
by
high
throughput
bisulfite
sequencing
in
tissue
samples
(nodule
size
<
3
cm
diameter)
learn
patterns
that
cancerous
lesions.Then
we
filtered
out
exhibiting
background
circulating
tumor
(ctDNA)
built
an
assay
plasma
sample
classification.Results:
first
of
230
cancer-specific
achieved
sensitivity
92.7%
(88.3%
-97.1%)
specificity
92.8%
(89.3%
-96.3%).These
tissue-derived
markers
were
further
using
training
set
66
9
selected
build
prediction
model.From
independent
validation
additional
samples,
this
model
obtained
79.5%
(63.5%
-90.7%)
85.2%
(66.3%
-95.8%)
differentiating
patients
with
(n
=
39)
lesions
27).Additionally,
when
tested
on
gender
age
matched
asymptomatic
normal
individuals
118),
our
93.2%
(89.0%-98.3%).Specifically,
is
highly
sensitive
towards
cancer,
75.0%(55.0%-90.0%)
20
stage
Ia
cancer
85.7%
(57.1%-100.0%)
7
Ib
patients.
Conclusions:We
have
developed
novel
blood
based
detecting
early
as
well
nodules.
Cancer Cell,
Год журнала:
2021,
Номер
40(1), С. 26 - 35
Опубликована: Дек. 23, 2021
Functional
precision
medicine
is
a
strategy
whereby
live
tumor
cells
from
affected
individuals
are
directly
perturbed
with
drugs
to
provide
immediately
translatable,
personalized
information
guide
therapy.
The
heterogeneity
of
human
cancer
has
led
the
realization
that
approaches
needed
improve
treatment
outcomes.
Precision
oncology
traditionally
used
static
features
dictate
which
therapies
should
be
used.
Static
can
include
expression
key
targets
or
genomic
analysis
mutations
identify
therapeutically
targetable
"drivers."
Although
surprisingly
small
proportion
derive
clinical
benefit
approach,
functional
additional
regarding
vulnerabilities.
We
discuss
emerging
technologies
for
as
well
limitations
and
challenges
in
using
these
assays
trials
will
necessary
determine
whether
outcomes
eventually
become
standard
tool
oncology.
