Global abundance patterns, diversity, and ecology of Patescibacteria in wastewater treatment plants

Microbiome, Год журнала: 2024, Номер 12(1)

Опубликована: Март 16, 2024

Microorganisms are responsible for nutrient removal and resource recovery in wastewater treatment plants (WWTPs), their diversity is often studied by 16S rRNA gene amplicon sequencing. However, this approach underestimates the abundance of Patescibacteria due to low coverage commonly used PCR primers highly divergent bacterial phylum. Therefore, our current understanding global diversity, distribution, ecological role WWTPs very incomplete. This particularly relevant as considered be associated with microbial host cells can therefore influence temporal variability other groups that important WWTP functioning.

Язык: Английский

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Core Microbiome of Medicinal Plant Salvia miltiorrhiza Seed: A Rich Reservoir of Beneficial Microbes for Secondary Metabolism?

International Journal of Molecular Sciences, Год журнала: 2018, Номер 19(3), С. 672 - 672

Опубликована: Фев. 27, 2018

Seed microbiome includes special endophytic or epiphytic microbial taxa associated with seeds, which affects seed germination, plant growth, and health. Here, we analyzed the core of 21 Salvia miltiorrhiza seeds from seven different geographic origins using 16S rDNA ITS amplicon sequencing, followed by bioinformatics analysis. The whole bacterial was classified into 17 phyla 39 classes. Gammaproteobacteria (67.6%), Alphaproteobacteria (15.6%), Betaproteobacteria (2.6%), Sphingobacteria (5.0%), Bacilli (4.6%), Actinobacteria (2.9%) belonged to microbiome. Dothideomycetes comprised 94% fungal in S. another two dominant classes were Leotiomycetes (3.0%) Tremellomycetes (2.0%). We found that terpenoid backbone biosynthesis, degradation limonene, pinene, geraniol, prenyltransferases, overrepresented phylogenetic examination communities reconstruction unobserved states (PICRUSt) software. also genera Pantoea, Pseudomonas, Sphingomonas enriched overlapped among miltiorrhiza, maize, bean, rice, while a genus, Alternaria, shared within Brassicaceae families. These findings highlight seed-associated microbiomeis an important component microbiomes, may be gene reservoir for secondary metabolism medicinal plants.

Язык: Английский

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Extensive Core Microbiome in Drone-Captured Whale Blow Supports a Framework for Health Monitoring

mSystems, Год журнала: 2017, Номер 2(5)

Опубликована: Окт. 11, 2017

The pulmonary system is a common site for bacterial infections in cetaceans, but very little known about their respiratory microbiome. We used small, unmanned hexacopter to collect exhaled breath condensate (blow) from two geographically distinct populations of apparently healthy humpback whales (Megaptera novaeangliae), sampled the Massachusetts coastal waters off Cape Cod (n = 17) and around Vancouver Island 9). Bacterial archaeal small-subunit rRNA genes were amplified sequenced blow samples, including many sparse volume, as well seawater other controls, characterize associated microbial community. microbiomes included 25 phylogenetically diverse bacteria all whales. This core assemblage comprised on average 36% microbiome, making it one more consistent animal studied date. closest phylogenetic relatives 20 these microbes previously detected marine mammals, suggesting that this microbiome specialized mammals may indicate healthy, noninfected system. Pathogen screening was conducted at genus level, which showed few contained pathogens; no cetacean pathogens blow. Overall, discovery shared large an important advancement health disease monitoring species IMPORTANCE conservation management rely part upon individuals populations, methods generally necessitate invasive sampling. Here, we drone noninvasively fly above capture (blow), examine In first extensive examination large-whale present surprising results across individual separated ocean basins. suggest addition characteristics, could be useful feature worldwide.

Язык: Английский

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Defining the core microbiome of the symbiotic dinoflagellate, Symbiodinium

Environmental Microbiology Reports, Год журнала: 2017, Номер 10(1), С. 7 - 11

Опубликована: Ноя. 10, 2017

Summary Dinoflagellates of the genus Symbiodinium underpin survival and ecological success corals. The use cultured strains has been particularly important to disentangle complex life history their contribution coral host physiology. However, these cultures typically harbour abundant bacterial communities which likely play important, but currently unknown, roles in biology. We characterized living association with a wide phylogenetic diversity (18 types spanning 5 clades) define core microbiome. Similar other systems, bacteria were nearly two orders magnitude more numerically than cells we identified three operational taxonomic units (OTUs) present all cultures. These represented α‐proteobacterium Labrenzia γ‐proteobacteria Marinobacter Chromatiaceae . Based on abundance functional potential harboured cultures, physiology can no longer be ignored.

Язык: Английский

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Modularity and predicted functions of the global sponge-microbiome network

Nature Communications, Год журнала: 2019, Номер 10(1)

Опубликована: Март 1, 2019

Defining the organisation of species interaction networks and unveiling processes behind their assembly is fundamental to understanding patterns biodiversity, community stability ecosystem functioning. Marine sponges host complex communities microorganisms that contribute health survival, yet mechanisms microbiome are largely unknown. We present global marine sponge-microbiome network reveal a modular in both structure function. Modules linked by few sponge share microbes with other around world. Further, we provide evidence abiotic factors influence structuring when considering all present, but biotic interactions drive more intimately associated 'core' microorganisms. These findings suggest ecological evolutionary at play host-microbe assembly. expect be consistent across multicellular hosts throughout tree life.

Язык: Английский

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