Thiolated chitosan nanoparticles encapsulated nisin and selenium: antimicrobial/antibiofilm/anti-attachment/immunomodulatory multi-functional agent

BMC Microbiology, Год журнала: 2024, Номер 24(1)

Опубликована: Июль 12, 2024

Abstract Background The increase in the resistance of bacterial strains to antibiotics has led research into bactericidal potential non-antibiotic compounds. This study aimed evaluate vitro antibacterial/ antibiofilm properties nisin and selenium encapsulated thiolated chitosan nanoparticles (N/Se@TCsNPs) against prevalent enteric pathogens including standard isolates Vibrio (V.) cholerae O1 El Tor ATCC 14,035, Campylobacter (C.) jejuni 29,428, Salmonella (S.) enterica subsp. 19,430, Shigella dysenteriae PTCC 1188, Escherichia (E.) coli O157:H7 25,922, Listeria (L.) monocytogenes 19,115, Staphylococcus aureus 29,733. Methods synthesis comprehensive analysis N/Se@TCsNPs have been completed. Antibacterial capabilities were evaluated through broth microdilution crystal violet assays. Furthermore, included examining cytotoxic effects on Caco-2 cells exploring immunomodulatory N/Se@TCsNPs. assessing levels both pro-inflammatory (IL-6 TNFα) anti-inflammatory (IL-10 TGFβ) cytokines determining gene expression TLR2 TLR4 . Results showed an average diameter 136.26 ± 43.17 nm a zeta 0.27 0.07 mV. FTIR spectroscopy validated structural features Scanning electron microscopy (SEM) images confirmed their spherical shape uniform distribution. Thermogravimetric Analysis (TGA)/Differential Calorimetry (DSC) tests demonstrated thermal stability N/Se@TCsNPs, showing minimal weight loss 0.03%±0.06 up 80 °C. prepared thiol content 512.66 7.33 µmol/g ( p < 0.05), encapsulation efficiency (EE) 69.83%±0.04 ≤ 0.001), drug release rate 74.32%±3.45 at pH = 7.2 0.004). synthesized nanostructure potent antibacterial activity various isolates, with effective concentrations ranging from 1.5 0.08 25 4.04 mg/mL. ability reduce adhesion internalization underscored 0.0001). Immunological studies indicated that treatment decreased inflammatory IL-6 (14.33 2.33 pg/mL) TNFα (25 0.5 0.0001), alongside increased IL-10 (46.00 0.57 TGFβ (42.58 2.10 infected Moreover, significantly reduced (0.22 0.09) (0.16 0.05) Conclusion In conclusion, exhibited significant antibacterial/antibiofilm/anti-attachment/immunomodulatory effectiveness selected Gram-positive Gram-negative pathogens. However, additional ex-vivo in-vivo investigations are needed fully assess performance nanostructured

Язык: Английский

Nano delivery platforms for nisin, a potent cyclic peptide: a comprehensive review of biological applications

Polymer Bulletin, Год журнала: 2025, Номер unknown

Опубликована: Март 17, 2025

Язык: Английский

Inhibitory Effect of DNase–Chitosan–Nisin Nanoparticles on Cell Viability, Motility, and Spatial Structures of Listeria monocytogenes Biofilms

Foods, Год журнала: 2024, Номер 13(22), С. 3544 - 3544

Опубликована: Ноя. 6, 2024

biofilm contamination on food contact surfaces is a major concern for the industry. Nanoparticle encapsulation appears as novel strategy surface disinfection to prevent formation. Chitosan nanoparticles loaded with nisin and DNase I (DNase-CS-N) have been constructed exhibit antimicrobial activity against

Язык: Английский