Identification and Anti-Fatigue Activity of Walnut Protein Hydrolysate

Nutrients, Год журнала: 2025, Номер 17(6), С. 1002 - 1002

Опубликована: Март 12, 2025

Objective: This study aims to investigate the structural and functional characteristics of walnut protein hydrolysates (WPHs) with different molecular weights prepared using protease from Dregea sinensis Hemsl, as well anti-fatigue effects low-molecular-weight (LWPs) their impact on cecal microbiota faecal metabolism mice. Methods: The activity WPHs was evaluated, LWPs were analyzed in a centralized manner. A 28-day gavage conducted assess LWP’s benefits mice, supplemented by metabolomic analysis explore its metabolic pathways. Results: Our findings revealed that LWP significantly outperformed unhydrolyzed (WP) terms water retention, lipid emulsifying properties, foaming capacity. Notably, differential expression associated highlighted pathways related antioxidant activity. In vivo studies showed markedly enhanced glycogen storage muscles liver while reducing serum levels urea nitrogen, lactate dehydrogenase, blood lactic acid, creatine kinase. Furthermore, Superoxide Dismutase Glutathione elevated, alongside reduction Malondialdehyde, indicating effect is closely linked improved oxidative stress resistance. Additionally, promoted beneficial increases microbial populations such Akkermansia, Alistipes, Eubacterium, Muribaculum, which are fatigue Metabolomic indicated significant enrichment glycerophospholipid amino acid biosynthesis, identifying key metabolites including palmitoylethanolamide 4-methyl-5-thiazoleethanol, both integral health maintenance. Conclusions: demonstrates robust effect, supported accessibility, straightforward preparation, eco-friendly characteristics. These attributes suggest has promising potential for inclusion products aimed at enhancing vitality combating fatigue.

Язык: Английский

Separation and characterization of sweet compounds in Baijiu by molecular distillation combined with molecular sensory science

Food Chemistry, Год журнала: 2024, Номер 465, С. 142124 - 142124

Опубликована: Ноя. 19, 2024

Язык: Английский