Unravelling the Biological Interplay Between Genital HPV Infection and Cervicovaginal Microbiota in Sub-Saharan Africa: Implications for Cervical (Pre)cancer Prevention DOI Creative Commons
Harris Onywera, Zizipho Z. A. Mbulawa, Adrian Brink

и другие.

Venereology, Год журнала: 2024, Номер 3(4), С. 211 - 231

Опубликована: Ноя. 14, 2024

Cervical cancer is more common in Sub-Saharan Africa (SSA) compared to developed countries, with persistent genital high-risk HPV (HR-HPV) infection identified as the cause. However, other factors contributing this gap remain unclear. This review explores potential role of cervicovaginal microbiota (CVM) and cervical development among women SSA. Many African descent, including those from SSA, lack lactobacilli dominance their CVM, which considered a biomarker health. Published literature has associated Lactobacillus-dominated CVM lower risk cancer. The converse true for high diversity non-Lactobacillus-dominated bacterial vaginosis, most form vaginal disorder. findings on relationship between specific abundance disease severity are inconsistent inconclusive due differences study design, population, sampling, confounders. Thus, there need consensus advance research HPV-associated disease. Despite exact mechanisms by influence HR-HPV persistence carcinogenesis being unknown, backbone appears be mediated part following: dysbiosis, elevated pH, ratio L-to-D-lactic acid, cohesive biofilm formation, chronic inflammation, immune dysregulation. Consequently, these promote cellular proliferation, genetic instability, evasion surveillance. calls larger, prospective studies unravel causal links, identify protective features, integrate interventions into prevention strategies

Язык: Английский

Fighting polymicrobial biofilms in bacterial vaginosis DOI Creative Commons
Lúcia G. V. Sousa, Sofia Pereira, Nuno Cerca

и другие.

Microbial Biotechnology, Год журнала: 2023, Номер 16(7), С. 1423 - 1437

Опубликована: Апрель 12, 2023

Abstract Bacterial vaginosis (BV) is the most common cause of vaginal discharge and often associated with other health consequences mainly in pregnant women. BV described by an imbalance microbiota where strictly facultative anaerobic bacteria outgrow lactic acid‐ hydrogen peroxide‐producing Lactobacillus species. The species involved are capable to grow form a polymicrobial biofilm epithelium. treatment usually performed using broad‐spectrum antibiotics, including metronidazole clindamycin. However, these conventional treatments high recurrence rates. may have important role on outcome accounted as one factors for failure. Other possible reasons failure include presence resistant antibiotics or chance reinfection after treatment. Therefore, novel strategies increase rates been studied namely use probiotics prebiotics, acidifying agents, antiseptics, plant‐based products, transplantation, phage endolysins. Although some them still initial phase development very preliminary results, they show great perspectives application. In this review, we aimed study nature explore few alternatives

Язык: Английский

Процитировано

32
Advances in bacteriophage-mediated strategies for combating polymicrobial biofilms DOI Creative Commons
Marta Gliźniewicz, Dominika Miłek, Patrycja Olszewska

и другие.

Frontiers in Microbiology, Год журнала: 2024, Номер 14

Опубликована: Янв. 5, 2024

Bacteria and fungi tend to coexist within biofilms instead of in planktonic states. Usually, such communities include cross-kingdom microorganisms, which make them harder remove from abiotic surfaces or infection sites. Additionally, the produced biofilm matrix protects embedded microorganisms antibiotics, disinfectants, host immune system. Therefore, classic therapies based on antibiotics might be ineffective, especially when multidrug-resistant bacteria are causative factors. The complexities surrounding eradication diverse human body have spurred exploration alternative therapeutic modalities. Among these options, bacteriophages their enzymatic counterparts emerged as promising candidates, either employed independently synergy with other agents. Phages natural killers because mechanisms action that differ phages answer worldwide problems bacterial infections. In this review, we report attempts use combating polymicrobial vitro studies, using different models, including therapeutical phages. addition, sum up advantages, disadvantages, perspectives phage therapy.

Язык: Английский

Процитировано

14
Key bacterial vaginosis-associated bacteria influence each other’s growth in biofilms on rich media and media simulating vaginal tract secretions DOI Creative Commons
Lúcia G. V. Sousa, Christina A. Muzny, Nuno Cerca

и другие.

Biofilm, Год журнала: 2025, Номер 9, С. 100247 - 100247

Опубликована: Янв. 5, 2025

Bacterial vaginosis (BV) is a very common gynaecologic condition affecting women of reproductive age worldwide. BV characterized by depletion lactic acid-producing Lactobacillus species and an increase in strict facultative anaerobic bacteria that develop polymicrobial biofilm on the vaginal epithelium. Despite multiple decades research, etiology this infection still not clear. However, some BV-associated (BVAB) may play key role development infection, namely Gardnerella species, Prevotella bivia, Fannyhessea vaginae. In work, we aimed to characterize growth these three rich medium simulating tract secretions (mGTS). We first assessed planktonic New York City (NYCIII) mGTS observed showed distinct capacities grow two media. Surprisingly, despite ability all single-species NYCIII, triple-species consortium P. bivia was able its concentration after 48 h, as qPCR. Furthermore, when using more restrictive media, G. vaginalis only BVAB consortia. Interestingly, found NYCIII influenced cell-free supernatant (CFS) F. vaginae CFS mGTS. This antimicrobial activity appears happen due acidification Single- biofilms were then formed, each further quantified While had high capacity form both favored cultured Differences also structure formed confocal laser scanning microscopy. conclusion, while essential for incorporation other biofilm.

Язык: Английский

Процитировано

2
3D printed reservoir-like vaginal rings for antibiotic delivery DOI
Arianna Chiappa,

Alice Fusari,

Marco Uboldi

и другие.

International Journal of Pharmaceutics, Год журнала: 2025, Номер unknown, С. 125217 - 125217

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

2
Probiotics: A New Approach for the Prevention and Treatment of Cervical Cancer DOI
Hui Li, Zhen Xu, Chaoqi Liu

и другие.

Probiotics and Antimicrobial Proteins, Год журнала: 2025, Номер unknown

Опубликована: Фев. 14, 2025

Язык: Английский

Процитировано

1
Dual probiotic and antibiotic therapy targeting bacterial vaginosis: an integrated experimental/computational modeling perspective DOI Creative Commons
Bassam Fotouh, Anthony J. Kyser, Mohamed Y. Mahmoud

и другие.

Biomedical Engineering Advances, Год журнала: 2025, Номер unknown, С. 100163 - 100163

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

1
Recent development and fighting strategies for lincosamide antibiotic resistance DOI
Yingying Yang,

Shiyu Xie,

Fangjing He

и другие.

Clinical Microbiology Reviews, Год журнала: 2024, Номер 37(2)

Опубликована: Апрель 18, 2024

SUMMARYLincosamides constitute an important class of antibiotics used against a wide range pathogens, including methicillin-resistant

Язык: Английский

Процитировано

6
The microbiome in recurrent pregnancy loss – A scoping review DOI Creative Commons
Kilian Vomstein, Maria Christine Krog, Tine Wrønding

и другие.

Journal of Reproductive Immunology, Год журнала: 2024, Номер 163, С. 104251 - 104251

Опубликована: Апрель 25, 2024

Recurrent pregnancy loss (RPL) is a troubling condition that affects couples worldwide. Despite extensive research efforts, many RPL cases remain unexplained, highlighting the need for novel approaches to unravel its underlying mechanisms. Recent advances in microbiome have shed light on potential role of reproductive health and outcomes. Based systematic literature research, this review aims comprehensively explore current understanding microbiome's involvement RPL, focusing vaginal, endometrial, gut microbiomes. Evidence from available studies examined explain relationship between RPL. Furthermore, we discuss diagnostic microbiome, therapeutic interventions, future directions Understanding complex interactions holds promise developing targeted interventions help patients today diagnosed as unexplained.

Язык: Английский

Процитировано

6
Vaginal dysbiosis and the potential of vaginal microbiome-directed therapeutics DOI Creative Commons
Valerie Diane Valeriano, Emilia Lahtinen, In‐Chan Hwang

и другие.

Frontiers in Microbiomes, Год журнала: 2024, Номер 3

Опубликована: Окт. 11, 2024

A healthy vaginal microbiome (VMB) is dominated by Lactobacillus spp. and provides the first line of defense against invading pathogens. Vaginal dysbiosis, characterized loss dominance increase microbial diversity, has been linked to an increased risk adverse genital tract diseases, including bacterial vaginosis, aerobic vaginitis, vulvovaginal candidiasis, sexually transmitted infections, pregnancy complications such as preterm birth. Currently, antibiotics antifungals are recommended first-line treatments with high cure rates, but they also can lead recurrence resistance development. As alternative, lactobacilli have utilized restore microbiota. In this review article, we discuss dysbiosis in various gynecological infections potential interventions based on Live Biotherapeutic Products (LBPs) a focus those that use intravaginal treatment modalities modulate VMB. Based these, provide insights key factors consider designing phenotypic genotypic screens for selecting strains vaginally administered microbiome-directed therapeutics. Lastly, highlight current progress within field, overview LBPs currently being developed published clinical trial completion recurrent BV, VVC, UTI. We regulatory challenges drug development process harmonize future research efforts VMB therapy.

Язык: Английский

Процитировано

5
Antibiofilm Agents for the Treatment and Prevention of Bacterial Vaginosis: A Systematic Narrative Review DOI Creative Commons

Michael Gao,

Jim Manos, Greg Whiteley

и другие.

The Journal of Infectious Diseases, Год журнала: 2024, Номер 230(3), С. e508 - e517

Опубликована: Апрель 29, 2024

Bacterial vaginosis (BV) is difficult to eradicate due BV biofilms protecting bacteria (Gardnerella, Prevotella, and other genera). With the growing understanding of biofilms, we systematically reviewed current knowledge on efficacy anti-BV biofilm agents.

Язык: Английский

Процитировано

5