Genetic Dissection of Cyclic di‐GMP Signalling in Pseudomonas aeruginosa via Systematic Diguanylate Cyclase Disruption DOI Creative Commons
Román A. Martino, Daniel C. Volke, Albano H. Tenaglia

и другие.

Microbial Biotechnology, Год журнала: 2025, Номер 18(4)

Опубликована: Апрель 1, 2025

The second messenger bis-(3' → 5')-cyclic dimeric guanosine monophosphate (c-di-GMP) governs adaptive responses in the opportunistic pathogen Pseudomonas aeruginosa, including biofilm formation and transition from acute to chronic infections. Understanding intricate c-di-GMP signalling network remains challenging due overlapping activities of numerous diguanylate cyclases (DGCs). In this study, we employed a CRISPR-based multiplex genome-editing tool disrupt all 32 GGDEF domain-containing proteins (GCPs) implicated P. aeruginosa PA14. Phenotypic physiological analyses revealed that resulting mutant was unable form biofilms had attenuated virulence. Residual levels were still detected despite extensive GCP disruption, underscoring robustness regulatory network. Taken together, these findings provide insights into complex metabolism showcase importance functional bacterial signalling. Moreover, our approach overcomes native redundancy synthesis, providing framework dissect individual DGC functions paving way for targeted strategies address adaptation pathogenesis.

Язык: Английский

The Legalome: Microbiology, Omics and Criminal Justice DOI Creative Commons
Alan C. Logan, Pragya Mishra, Susan L. Prescott

и другие.

Microbial Biotechnology, Год журнала: 2025, Номер 18(3)

Опубликована: Март 1, 2025

ABSTRACT Advances in neuromicrobiology and related omics technologies have reinforced the idea that unseen microbes play critical roles human cognition behaviour. Included this research is evidence indicating gut microbes, through direct indirect pathways, can influence aggression, anger, irritability antisocial Moreover, manufacture chemicals are known to compromise cognition. For example, recent court decisions United States Europe acknowledge produce high levels of ethanol, without consumption alcohol by defendants. The dismissal driving while intoxicated charges these cases—so‐called auto‐brewery syndrome—highlights way which microbiome knowledge will enhance precision, objectivity fairness our legal systems. Here opinion essay, we introduce concept ‘legalome’—the application science forensic psychiatry criminal law. We argue rapid pace microbial discoveries, including those challenge ideas free moral responsibility, necessitate a reconsideration traditional doctrines justifications retributive punishment. implications extend beyond courtroom, challenging us reconsider how environmental factors—from diet socioeconomic conditions—might shape preventative rehabilitative efforts their effects on microbiome.

Язык: Английский

Процитировано

0
Aggression and Justice Involvement: Does Uric Acid Play a Role? DOI Creative Commons

Alan Logan,

Pragya Mishra

Brain Sciences, Год журнала: 2025, Номер 15(3), С. 268 - 268

Опубликована: Март 2, 2025

The search for biological markers that can be reliably linked to aggression and antisocial behavior has been central the work of criminology. One such marker, uric acid, long suspected play a causative role in promoting anger, irritability, aggression, violence. Here, this perspective article, we revisit some historical interest acid as compound relevant brain behavior, reflect these early accounts off emergent scientific research. Advances sciences, including neuropsychiatry neuromicrobiology, have allowed more sophisticated understanding potential mechanistic pathways linking with cognition behavior. updated science suggests ideas surrounding criminology had credibility. available research strongly biomarker risk, is worthy further close scrutiny. Informed by gut–brain–microbiome research, argue certain aspects early-to-mid-20th-century were prematurely abandoned. From legalome perspective, advances other gut–brain biomarkers aid shaping humane, scientifically grounded policies recognize interplay between biology environment.

Язык: Английский

Процитировано

0
Genetic Dissection of Cyclic di‐GMP Signalling in Pseudomonas aeruginosa via Systematic Diguanylate Cyclase Disruption DOI Creative Commons
Román A. Martino, Daniel C. Volke, Albano H. Tenaglia

и другие.

Microbial Biotechnology, Год журнала: 2025, Номер 18(4)

Опубликована: Апрель 1, 2025

The second messenger bis-(3' → 5')-cyclic dimeric guanosine monophosphate (c-di-GMP) governs adaptive responses in the opportunistic pathogen Pseudomonas aeruginosa, including biofilm formation and transition from acute to chronic infections. Understanding intricate c-di-GMP signalling network remains challenging due overlapping activities of numerous diguanylate cyclases (DGCs). In this study, we employed a CRISPR-based multiplex genome-editing tool disrupt all 32 GGDEF domain-containing proteins (GCPs) implicated P. aeruginosa PA14. Phenotypic physiological analyses revealed that resulting mutant was unable form biofilms had attenuated virulence. Residual levels were still detected despite extensive GCP disruption, underscoring robustness regulatory network. Taken together, these findings provide insights into complex metabolism showcase importance functional bacterial signalling. Moreover, our approach overcomes native redundancy synthesis, providing framework dissect individual DGC functions paving way for targeted strategies address adaptation pathogenesis.

Язык: Английский

Процитировано

0