bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Ноя. 5, 2023
Abstract
It
has
been
demonstrated
that
short-term
stress
can
enhance
cellular
responses
and
promote
longevity,
whereas
long-term
shortens
lifespan.
Understanding
the
relationship
between
could
offer
new
insights
into
comprehending
modulating
age-related
diseases.
In
this
study,
we
investigate
using
transcriptomic
metabolomic
analyses
in
yeast
model
system
(
Saccharomyces
cerevisiae
).
We
employed
three
metabolic
treatments:
firstly,
treating
cells
with
threshold
levels
of
benzoic
acid
for
24
hours
(Short-term
[ST]
Stressed
Cells);
secondly,
500
hours,
sub-culturing
every
(Long-term
[LT]
thirdly,
allowing
stressed
to
grow
16
without
any
(Recovered
Cells).
Here,
propose
aging
is
an
evolutionarily
conserved
adaptation
mechanism
response
exposure.
Under
conditions,
prominent
lifespan-extending
metabolites
such
as
trehalose
linked
tumor
suppression
humans,
5’-methylthioadenosine,
were
overexpressed.
contrast,
LT
Cells
activated
genes
those
responsible
epigenetic
regulatory
enzymes
govern
process,
secondary
genes,
heat
shock
proteins
(HSPs)
which
are
associated
cell
damage
but
also
often
aged
cells.
Chronological
lifespan
experiments
showed
lived
a
shorter
compared
ST
Cells.
This
suggests
markers
(eg.
HSPs,
certain
regulators)
expressed
enable
survival
have
effect
reducing
support
hypothesis,
show
exclusively
solely
eukaryotes,
while
significantly
(aging
related)
exhibit
high
conservation
across
all
domains
life,
majority
having
originated
from
bacteria
hinting
at
potential
evolutionary
benefit
aging.
Abstract
Supplementation
with
S-adenosylhomocysteine
(SAH)
extends
the
lifespan
of
model
organisms.
To
explore
impact
SAH
on
aging,
we
generated
a
Caenorhabditis
elegans
by
introducing
hydrolase
(AHCY-1)
variant
Y145C,
corresponding
to
human
AHCY
Y143C
pathogenic
mutation.
This
mutation
is
anticipated
impair
hydrolysis,
resulting
in
its
increased
levels.
Our
findings
revealed
that
animals
this
endogenous
exhibited
delayed
accompanied
decreased
S-adenosylmethionine
(SAM)
and
moderately
The
extended
these
worms
depends
AMP-activated
protein
kinase
(AMPK),
activator
Vaccinia
virus–related
(VRK-1),
DAF-16
transcription
factor.
results
underline
complex
nature
SAH’s
influence
proposing
balance
between
SAM
might
play
pivotal
role
defining
C.
.
Moreover,
our
partial
AHCY-1
deficiency
offers
tool
for
studying
intersection
methionine
metabolism
aging.
Abstract
Sake
yeasts
have
a
range
of
brewing
characteristics
that
are
particularly
beneficial
for
sake
making
including
high
ethanol
fermentability,
proliferative
capacity
at
low
temperatures,
lactic
acid
tolerance,
and
ester
productivity.
On
the
other
hand,
also
accumulate
diverse
functional
components.
For
example,
significantly
greater
accumulation
S-adenosylmethionine
(SAM),
compound
plays
important
regulatory
roles
in
biological
processes
as
major
donor
methyl
groups,
occurs
compared
to
microorganisms.
Significantly
folate,
bioactive
water-soluble
vitamin
(vitamin
B9),
laboratory
yeasts,
group
on
SAM
is
supplied
by
folate.
Accordingly,
fully
characterizing
‘sake
yeast
identity’
requires
detailed
understanding
mechanisms
underlying
both
nutritional
(functional
components)
yeasts.
Therefore,
this
mini-review
focuses
folate
descriptions
genes
known
contribute
mechanisms.
Research Square (Research Square),
Год журнала:
2023,
Номер
unknown
Опубликована: Апрель 26, 2023
Abstract
Supplementation
with
S-adenosylhomocysteine
(SAH)
extends
the
lifespan
of
model
organisms.
To
explore
impact
SAH
on
aging,
we
generated
a
Caenorhabditis
elegans
increased
levels
through
pathogenic
mutation
in
hydrolase
(AHCY),
which
impairs
hydrolysis
to
adenosine
and
homocysteine.
We
showed
that
animals
an
endogenous
leading
AHCY
Y145C
exhibited
delayed
aging
accompanied
by
reduced
SAM
levels.
The
extended
longevity
these
required
AMP-activated
protein
kinase
(AMPK).
Our
results
demonstrate
potential
mutant
worms
as
tool
study
influence
point
SAM:SAH
ratio
determinant
C.
lifespan.
T
uber
indicum
is
the
most
economically
important
member
of
Tuber,
with
highest
production
and
widest
distribution
in
China.
However,
overexploitation
immature
ascocarps
not
only
have
driven
wild
resources
species
toward
extinction,
but
also
has
caused
enconomic
losses
a
decline
reputation
T.indicum
quality.
In
this
study,
stage-specific
metabolites
T.
relation
to
nutritional
quality
mechanism
their
accumulations
were
explored
by
transcriptome
metabolome
analysis
at
five
harvest
times,
representing
four
maturation
stages.
A
total
663
compounds
identified
widely
targeted
metabolomic
approach.
Lipid
are
prominent
(18%)
our
samples
higher
accumulation
stage
than
mature
stage,
30.16%
differential
accumulated
stage.
Levels
some
amino
acids,
such
as
S-(methyl)
glutathione,
S-adenosylmethionine,
which
known
truffle
aroma
precursors,
increased
The
gene
expression
level
related
biosynthesis
volatile
organic
verified
qPCR.
This
study
contributes
preliminary
understanding
variations
during
maturity
for
evaluation
biology.
T
uber
indicum
is
the
most
economically
important
member
of
Tuber,
with
highest
production
and
widest
distribution
in
China.
However,
overexploitation
immature
ascocarps
not
only
have
driven
wild
resources
species
toward
extinction,
but
also
has
caused
enconomic
losses
a
decline
reputation
T.indicum
quality.
In
this
study,
stage-specific
metabolites
T.
relation
to
nutritional
quality
mechanism
their
accumulations
were
explored
by
transcriptome
metabolome
analysis
at
five
harvest
times,
representing
four
maturation
stages.
A
total
663
compounds
identified
widely
targeted
metabolomic
approach.
Lipid
are
prominent
(18%)
our
samples
higher
accumulation
stage
than
mature
stage,
30.16%
differential
accumulated
stage.
Levels
some
amino
acids,
such
as
S-(methyl)
glutathione,
S-adenosylmethionine,
which
known
truffle
aroma
precursors,
increased
The
gene
expression
level
related
biosynthesis
volatile
organic
verified
qPCR.
This
study
contributes
preliminary
understanding
variations
during
maturity
for
evaluation
biology.
Journal of Clinical Biochemistry and Nutrition,
Год журнала:
2023,
Номер
73(3), С. 173 - 177
Опубликована: Янв. 1, 2023
The
molecular
mechanism
of
aging,
which
has
been
a
“black
box”
for
many
years,
elucidated
in
recent
and
the
nematode
C.
elegans,
is
model
animal
aging
research,
played
major
role
its
elucidation.
From
analysis
elegans
longevity-related
mutant
genes,
signal
transduction
systems,
with
insulin/insulin-like
growth
factor
system
at
core,
have
emerged.
It
become
clear
that
this
greatly
affected
by
external
nutrients
involved
downstream
regulation
oxidative
stress,
considered
to
be
one
main
causes
aging.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Ноя. 5, 2023
Abstract
It
has
been
demonstrated
that
short-term
stress
can
enhance
cellular
responses
and
promote
longevity,
whereas
long-term
shortens
lifespan.
Understanding
the
relationship
between
could
offer
new
insights
into
comprehending
modulating
age-related
diseases.
In
this
study,
we
investigate
using
transcriptomic
metabolomic
analyses
in
yeast
model
system
(
Saccharomyces
cerevisiae
).
We
employed
three
metabolic
treatments:
firstly,
treating
cells
with
threshold
levels
of
benzoic
acid
for
24
hours
(Short-term
[ST]
Stressed
Cells);
secondly,
500
hours,
sub-culturing
every
(Long-term
[LT]
thirdly,
allowing
stressed
to
grow
16
without
any
(Recovered
Cells).
Here,
propose
aging
is
an
evolutionarily
conserved
adaptation
mechanism
response
exposure.
Under
conditions,
prominent
lifespan-extending
metabolites
such
as
trehalose
linked
tumor
suppression
humans,
5’-methylthioadenosine,
were
overexpressed.
contrast,
LT
Cells
activated
genes
those
responsible
epigenetic
regulatory
enzymes
govern
process,
secondary
genes,
heat
shock
proteins
(HSPs)
which
are
associated
cell
damage
but
also
often
aged
cells.
Chronological
lifespan
experiments
showed
lived
a
shorter
compared
ST
Cells.
This
suggests
markers
(eg.
HSPs,
certain
regulators)
expressed
enable
survival
have
effect
reducing
support
hypothesis,
show
exclusively
solely
eukaryotes,
while
significantly
(aging
related)
exhibit
high
conservation
across
all
domains
life,
majority
having
originated
from
bacteria
hinting
at
potential
evolutionary
benefit
aging.
